透析

The optimum work concentration of the indirect ELISA for detecting and screening positive hybridoma cells was established by research again and again. Polystyrene microtitration plates were coated with the purified DHV antigen at the amount of 0.5μg/well.

对鸡胚和鸭胚增殖的尿囊液病毒用反复冻融、氯仿处理、0.22μm滤膜过滤、PEG反透析浓缩以及Sephadex G-200分子筛层析的方法进行纯化，并以此来包被酶标板以及进行BALB/c小鼠免疫，效果良好。

The scFv formed inclusion body in E. Coli. 8 mol/L urea was used to solubilize the inclusion body, and the scFv was then purified in two steps , including Ni 2+ chelating affinity chromatography and gel filtration.

大肠杆菌表达的单链抗体包涵体，用8 mol/L尿素裂解，经过Ni离子螯合亲和层析和凝胶过滤两步纯化后，纯度达到97%以上，再通过透析复性除去尿素。

Coli BL21 (DE3) plysS for expression under induction of IPTG. The expressed product in a form of inclusion body was denaturalized with urea, purified by nickel ion chelate affinity chromatography, renaturalized by gradient dialysis and identified by Western blot.

coli BL21(DE3) plysS进行诱导表达，采用Ni(上标 2+)螯合亲和层析法纯化经尿素变性的包涵体，梯度透析复性纯化的目的蛋白，并进行Western blot鉴定。

You run the dialyzed solution over a size-exclusion chromatographic column.

你将透析了的溶液进行分子排阻层析。

You run the dialyzed solution over a size-exclusion chrom atographic column.

fractions 你将透析了的溶液进行分子排阻层析。根据方法，你收集柱子上出来的

PAO was purified by Ni-NTA resin under denature condition and dialyzed to recover its native structure. After that, the enzyme activity was analyzed with chemical fluorescent method.

IPTG 诱导表达的重组PAO经Ni-NTA亲和层析纯化和透析复性后，化学荧光法测定酶活性。

The invention uses the fermentation liquor of one Escherichia coli generating polysialic acid as material, filters and removes thallus, adds some solvents as sodium chloride and alcohol into supernatant to deposit polysialic acid, dissolves the polysialic acid deposits via deionized water, filters to remove modified protein, uses alkali prolease to treat filtrate, complexes deposits via cetyl pyridinium chloride or cetyltrimethylammonium bromide, and dissociates, uses over alcohol to deposit polysialic acid, dissolves the deposits via ultra-pure water, grades and purifies via chromatography column, collects and dialyzes eluent, cools and dries to obtain polysialic acid.

本发明以一株产聚唾液酸大肠杆菌的发酵液为原料，经过过滤去除菌体，上清液加入适量氯化钠和乙醇等溶剂沉淀出聚唾液酸，聚唾液酸沉淀用去离子水溶解，再用过滤去除变性蛋白质，滤液经过碱性蛋白酶处理、氯代十六烷基吡啶或十六烷基三甲基溴化铵络合沉淀并解离后，再用过量乙醇沉淀出聚唾液酸，聚唾液酸沉淀用超纯水溶解，用层析柱进行分级纯化，收集洗脱液经过透析，冷冻干燥得聚唾液酸产品。

The extract was purified further by vacuum filtering, salting-out, dialyzing and lyophilizing.

粗提液再经抽滤、盐析、透析，最后经冷冻乾燥，可得到纯度较高的胶原蛋白制品。

By this method, the productivity of 3. 18g/l extracellular polysaccharides was obtained. The optimum extraction method was obtained by RSA. It was determined as follows: 91. 4 ℃, 2. 9h, the weight ratio between mycelia and water was 1 to 3. The productivity was 12. 38% of dry mycelia. Set the purifying methods of Grifola frondosa: Precipitated polysaccharides part by 60% ethanol→removed protein by Sevag method→removed coloring matter by H〓O〓→removed salts by dialyse→DEAE-cellulose column chromatogram→ purified polysaccharides groups.

对于胞内多糖的提取，采用湿菌体经捣碎再高压破壁的方式破碎菌体细胞，采用响应面分析法得出优化后的提取条件为：提取温度91.4℃，提取时间2.9h，料水比1：3，此提取条件下胞内粗多糖的率为干菌体重的12.38％建立了灰树花多糖分离纯化的技术路线 60％乙醇沉淀的粗多糖→Sevag法脱蛋白→H〓O〓脱色→透析脱盐→DEAE-纤维素层析分离→多糖组分。

Methods Fresh adult bovine tendon was dissolved with acetic acid and enzyme-digestion for type Ⅰ collagen. Then they were dialysed and purified for refined liquid collagen, which was cross-linked with glutaraldehyde.

取新鲜成年牛跟腱，用乙酸溶解－酶消化法提取Ⅰ型胶原，并进行盐析、透析、纯化得到精制的液态胶原，用戊二醛进行交联。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。