透明质酸酶

Methods : The neural retina was isolated from RPE by enzyme one containing 220u/ml hyaluronidase and 0.1% trypsin, later the RPE cells was isolated from the Bruch"s membrane by enzyme two containing 0.25% trypsin, then the resembled RPE cells was injected into the host"s subretinal space with a special equipment after producing an artificial bleb detachment of neural retina.

用酶Ⅰ(含220u/ml透明质酸酶和0.1％胰蛋白酶)分离视网膜神经上皮层与RPE之间的联系，再用酶Ⅱ(含0.25％胰蛋白酶)将RPE从玻璃膜上分离下来获取悬浮的RPE细胞，用特制的移植针头通过人为造成的局限性视网膜脱离区将其移植入受体视网膜下间隙。

As well as working as an antioxidant that is moisturizing and healing to the skin, it stops the activity of the hyaluronidase, which will allow your skin to produce hyaluronic acid.

以及作为一种抗氧化剂是保湿和愈合的皮肤，它停止活动的透明质酸酶，这将使你的皮肤产生透明质酸。

Purpose To study the optimal reaction conditions of hyaluronic acid hydrolysis catalyzed by hyaluronidase.

目的 确定透明质酸酶催化透明质酸水解的最适条件。

MPS inhibits various aggressive lysosomal enzymes that degrade important constituents of connective tissue, such as elastase, collagenase and hyaluronidase.

MPS抑制了各种参与分解代谢的酶，这些酶用于降解结缔组织的重要组成元素，如弹性蛋白酶，胶原酶和透明质酸酶。

The first 0.1%collagenaseⅣ 0.1% hyaluronidase for 20 min and than 0.25% trypsin 0.04% EDTA 5μg/mL DNaseⅠfor 5 min,its enough to obtain germ cells and have optimal cell viability. 8.4±2.6×106 cells per gram testis were isolated and there was(19.7±5.3)% of PGP9.5 spermatogonium and (23.8±3.6)% of c-kit spermatogonium.

采用两步酶消化法，0.1%胶原酶和0.1%透明质酸酶消化20 min，0.25%胰酶 0.04% EDTA 5μg/mL DNaseⅠ处理5 min，就可以使睾丸生殖细胞充分分离处出来，每克睾丸组织克获得8.4±2.6×106个细胞，其中PGP9.5 精原细胞占(19.7±5.3)%，c-kit 精原细胞占总数的(23.8±3.6)%。

Methods: Isolated rat costalchondral growth plate by microdissection, dispersed RGC (rat costochondral growth plate chondrocyte) were obtained by digestion (0.05% hyaluronidase and 0.2% collagenase type Ⅱ), inoculated and cultured in monolayer....

显微解剖分离出大鼠肋生长板，酶消化法(0.05%透明质酸酶和0.2%Ⅱ型胶原酶)获得分散的大鼠肋生长板软骨细胞(rat costochondral growth plate chondrocyte，RGC)，常规接种，单层培养。

An enzyme that inactivates hyaluronic acid in the body,thereby increasing tissue permeability to fluids.

透明质酸酶一种能够降低体内透明质酸的活性，从而提高组织中液体渗透能力的酶

The pathogen of gas gangrene mainly exist and live in local place, and seldom enter blood system to cause blood poisoning, but than can form lots of toxins, this toxins can harm blood system, kidney system, and also the tissue can putrescence and the wound will enlarge step by step, all tissue putrescence and toxin can make these bacteria easier to live and reproduce, all these situation if not being control in time will lead a serious situation that multiple organs dysfunction and then died

气性坏疽的病原菌主要在伤口内生长繁殖很少侵入血液循环引起败血症。产气夹膜杆菌产生α毒素、胶原酶透明质酸酶、溶纤维酶和脱氧核糖核酸酶等，红细胞破坏引起溶血血红蛋白尿、尿少、肾组织坏死水肿、液化，肌肉大片坏死使病变迅速扩散、恶化。糖类分解产生大量气体使组织膨胀；蛋白质的分解和明胶的液化，产生硫化氢，使伤口发生恶臭由于局部缺血，血浆渗出，及各种毒素的作用伤口内的组织和肌肉，进一步坏死和腐化，更利于细菌的繁殖使病变更为恶化。

Several strategies have been applied to achieve this goal previously, such as lipidsome or polymer encapsulations, gene gun, in situ electroporation. Chemicals with a permeable...

结果证明油酸和透明质酸酶均能有效提高pCMVβ-gal在小鼠体内的表达量，其中透明质酸酶引起的增强效果更为显著，是较好的基因表达增强剂。

Methods TIL in the patients with PHC were separated with combinative enzymatic digestion of type Ⅰ,Ⅳ collagenases,type Ⅴ hyaluronidase and type Ⅰ deoxyribonuclease followed by discontinuous density gradients.Cytotoxicity of TIL to liver cancer cells was detected with MTT method.

采用Ⅰ型和Ⅳ型胶原酶、Ⅴ型透明质酸酶和Ⅰ型DNA酶四联酶消化，应用不连续密度梯度离心分离TIL，采用MTT比色法检测TIL对自体癌细胞及肝癌细胞株的细胞毒。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。