透明样化

Results Diagnosis of sebaceous carcinoma demanded immunohistochemistry, and rare parotid sebaceous carcinoma was still distinguished with clear cell carcinoma, grume epidermis carcinoma and acinic cell carcinoma in pathology. By Follow-up of the all patients over 5 years, survival rate for 5 years was 71.4%(20/28), primary focus recurrence in 10 cases, in which, neck lymph node metastasis was discovered in 3 cases, and one was performed operation once again. Four cases lost communication; 2cases died of distant metastasis; 2cases died of other diseases.

结果 皮脂腺癌确诊需要免疫组化检测，而罕见的腮腺皮脂腺癌还需和透明细胞癌、黏液表皮样癌和腺泡细胞癌鉴别，全组病例5年以上随访，5年生存率可达71.4%（20/28），5年内原发灶复发10例，其中，3例伴有区域淋巴结转移而再次手术，4例失访；2例死于远处转移；2例死于其他疾病。

Main HPV7(9/18) positive samples\' pathology manifestations matched the characteristics for HPV7 infection,which showed papillomatosis and acanthosis,deep-stained cells including keratohyalin granules in middle size,and obvious hyperkeratosis and parakertosis.

HPV7阳性(18份)的病理象特征：(1)乳头瘤样增生和棘层肥厚；(2)内含中等大小的透明角质颗粒的细胞深染；(3)显著的角化过度和角化不全。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

The cartilage of germinative layer and basal layer grew better than that in bfgf group, magnetic group.

结果表明：磁场结合bfgf组在4周时基底层的软骨样组织增生，而磁场组、bfgf组少量软骨样组织增生。8、12周结合组透明样软骨层明显增厚，免疫组化ⅱ型胶原多。

Methods 30 patients with trichloroethylene medicamentous dermatitis and 30 normal persons were selected as the observation group and the control group respectively; color Doppler was used to detect the spleen thickness and longitudinal length, spleen vein and artery diameters, the maximum blood velocity and blood flow of spleen vein and artery; RIA was applied to determine the serum levels of HA, LN, PCⅢ, CⅣ and CG; relevant analysis was made on the linear relationship between serum indices of hepatic fibrosis and spleen hemodynamics of the two groups.

选择30例三氯乙烯药疹样皮炎患者作为观察组，30例同时期健康体检者作为对照组，用彩色多普勒超声仪，检测脾脏厚度及长径、脾动静脉直径、脾动静脉血流量和脾静脉最大流速SPV(下标 x。用放射免疫法检测肝纤维化指标透明质酸、层粘连蛋白、Ⅲ型前胶原、Ⅳ型胶原和甘胆酸的水平，并对观察组与对照组的脾血流动力学参数与肝纤维化指标进行相关分析。

Results: All the slides of the breast carcinoma in our department were reviewed, 9 cases of glycogenich, clear cell carcinoma, 4 cases of mucinous carcinoma, 2 cases of tubular carcinoma, 18 cases of metaplastic carcinoma, 6 cases of apocrine carcinoma, 1 case of secretary carcinoma, and 2cases of lipid-rich carcinoma were found.

结果：回顾42例乳腺癌病理切片，发现富含糖原的透明细胞癌9例，黏液癌4例，小管癌2例，化生性癌18例，大汗腺样癌6例，分泌型癌1例，富脂质癌2例；不同组织类型的乳腺癌具有不同的光镜、特殊染色及电镜表现。

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色：翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成；基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质，在翼状胬肉体部深层基质中存在粗糙的纤维组织；正常球结膜组织细胞排列整齐；基质为疏松结缔组织，胶原纤维平行排列，其间可见成纤维细胞，散在少量中性粒细胞、毛细血管；Masson染色：翼状胬肉浅层基质中存在致密的胶原纤维染色，深层基质中的胶原纤维存在变性样改变；VG染色：翼状胬肉组织深层基质中存在大量变性的胶原纤维，其间夹杂黑色的弹性纤维；免疫组化染色法：MMP-3在翼状胬肉上皮细胞中呈强表达，成纤维细胞中呈中等强度表达，血管内皮细胞中未见表达；LN在血管壁中呈强表达，在上皮细胞基底膜中呈中等强度表达，在整个基质中未见明显表达；col Ⅲ在整个翼状胬肉基质中呈强表达。

Of all these patients, the eyelid in 10 cases, parotid in 2cases, external auditory meatus in 5 cases, maxillofacial in 7 cases, neck portion in 4 cases, neck lymph node metastasis in 3 cases. They were treated with surgical operation, radical therapy and chemistry therapy.

结果 皮脂腺癌确诊需要免疫组化检测，而罕见的腮腺皮脂腺癌还需和透明细胞癌、黏液表皮样癌和腺泡细胞癌鉴别，全组病例5年以上随访，5年生存率可达71.4%（20/28），5年内原发灶复发10例，其中，3例伴有区域淋巴结转移而再次手术，4例失访；2例死于远处转移；2例死于其他疾病。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。