透明带

Objective: To obtain the recombinant truncated porcine zona pellucida protein 3β(tunc- pZP3β) for further research in its function.

目的：制备具有猪卵透明带-3β抗原活性的截短型pZP3β蛋白(tunc-pZP3β)，以便对该蛋白的抗原表位区域作深入研究。

However, zona hardening could occur during in vitro maturation of oocytes in a simply defined medium which reduced the fertilization rate.

采用无蛋白培养基对卵母细胞进行体外成熟培养，并防止透明带硬化，是研究影响卵母细胞成熟过程因素的基础。

Immune POF model elicited by immunization in subcutaneous with antigen sourced isotype animal ovaries can cause growing follicles and mature follicles decrease obviously, the morphology of oocytes and zona pellucida abnormal, the adjacent granular cells disorder and atresic follicle increase.

以同种动物卵巢为抗原主动免疫建立的免疫性POF模型可致生长卵泡和成熟卵泡数明显减少，卵母细胞及透明带形态异常，周边颗粒细胞排列紊乱，闭锁卵泡增多，病理损伤随免疫时间延长而加重。

Methods of removing of zone pellucida and addition of growth factors to the culture medium had no effect on the efficiency of production of monozygotic twins.

这些结果表明，从F1品系可更有效地产生同卵孪生子；不同的去透明带方式和添加生长因子与否对产生同卵孪生小鼠的得率没有明显差异。

Increased motility, zona binding and oolemma fusion capacities are also acquired within the epididymis and are necessary for those cells that finally arrive at the site of fertilization.

精子在附睾中同时提高了运动力、透明带的结合能力以及与卵膜融合的能力，而这些能力是精子到达授精位置所必需的。

Methods A C57BL/6j mouse cumulus cell nucleus 10-12 mm in diameter was inserted into the perivitelline space of an enucleated oocyte.

方法将直径10~12mm的C57BL/6j小鼠卵丘细胞核注射到去核卵母细胞透明带下，构建供体核-卵母细胞复合体。

The giant panda-rabbit interspecies reconstructed embryos were constructed by injection of giant panda somatic cells into the perivitelline space of the enucleated oocytes and fused by electrical stimulation, and the reconstructed embryos can develop to hatched blastocyst stage in vitro. We demonstrated that the nuclear genetic materials in reconstructed blastocyst cells were the same as that of giant panda somatic cells by chromosome count and the existence of characteristic chromosome, which was confirmed by microsatellite DNA analysis later In order to determine the fate of mtDNA of giant panda-rabbit reconstructed embryos, we analyzed the cyt b sequence of embryos at 1 -cell stage, 2-cell stage, 4-cell stage, 8 cell stage, morula stage, blastocyst stage, expanded blastocyst stage and hatched blastocyst stage.

异种克隆大熊猫重构胚是通过将大熊猫成纤维细胞移入日本大耳白兔去核卵母细胞透明带下后经电融合构建的，可在体外发育至孵化囊胚阶段，我们应用一种简便、快速的异种重构胚的鉴定方法，即通过染色体数目和特征染色体的存在初步鉴定出大熊猫-兔异种重构胚的核遗传物质来源于大熊猫供核体细胞（后经昆明动物研究所通过微卫星DNA分析验证了该结果的可靠性）。

Do not cause decomposition of one ovulation, mature follicles to a certain stage, clearly highlighted in the ovarian surface, with the proliferation of follicular fluid, internal pressure increases, so that more and more prominent part of the ovarian tissue is thin, the final rupture secondary oocyte cells and their peripheral zona pellucida and corona radiata, together with the discharge of ovarian follicular fluid in the process called ovulation.

不排卵原因分解1、成熟卵泡发育到一定阶段，明显地突出于卵巢表面，随着卵泡液的激增，内压的升高，使突出部分的卵巢组织愈来愈薄，最后破裂次级卵母细胞及其外周的透明带和放射冠随卵泡液一起排出卵巢这一过程称排卵。

To establish a mouse microinsemination model, Intracytoplasmic sperm injection and subzonal sperm injection were studied use mouse gamets.

我们用小鼠卵细胞胞质内精子注射和透明带下精子注射的方法，对显微授精过程进行了初步探讨，对配子的采集和准备、精子的优化及显微注射的操作等作了阐述。

The results showed that the Ang II positive staining was observed not only in thecal cells , oocytes and zona pellucida of the follicles at different developmental stages but also in corpora lutea.

结果表明：Ang II分布在不同发育阶段卵泡的内、外膜细胞，卵母细胞及其透明带上，颗粒细胞和卵丘细胞未见着色。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。