选择性

The results prove that CNHK500 has highly proliferation selectivity and potent cytolysis effect on breast cancer in vitro.

在感染CNHK500的端粒酶阴性的正常成纤维细胞株中未检测到E1A基因表达，在端粒酶阳性的感染CNHK500的乳腺癌细胞株中能够检测到E1A基因的表达；CNHK500可以选择性地在缺氧条件下表达E1B。

CNHK500 can efficiently replicate in and kill liver cancer cells as well as wtAd5 do while it is severely attenuated in proliferation and cytolysis among normal cells.

结论实验结果表明CNHK500能有效地选择性在肝癌细胞中增殖、复制、杀伤，而在正常细胞中增殖和溶解细胞能力却大大减弱。

Methods: We used virus proliferation assay cell viability assay to evaluate the proliferation and cytolysis selectivity of CNHK500. And we used Western-blot to confirm the expression of adenovirus CNHK500 E1A and E1B in cancer and normal cells.

行病毒增殖实验和细胞生长抑制实验，验证CNHK500选择性复制和杀伤能力；Western blot检测腺病毒E1A和E1B在细胞中的表达。

Methods: Virus proliferation assay, cell viability assay and Western blot were performed to assess the selective replication and cytolysis of CNHK500 in telomerase positive liver cancer cells Hep3B, HepGⅡ, SMMC7721 and in normal cells.

方法利用病毒增殖实验、细胞活力实验、蛋白印迹分析来检测增殖病毒CNHK500在端粒酶阳性的肝癌细胞株HepGⅡ、Hep3B、SMMC7721及正常细胞中选择性增殖和溶解细胞的特性。

Antitumor efficacy of replication-competent adenovirus CNHK500 in vivo was associated with increased dosage of CNHK500. Conclusions: The results prove that CNHK500 has highly proliferation selectivity and potent cytolysis effect on breast cancer.

肿瘤选择性增殖腺病毒CNHK500可选择性在端粒酶阳性的乳腺癌细胞中复制，并产生体内外杀伤作用。

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

Objective: To determine the potency of QN-2013, a derivative of quinoxaline 1,4-N-oxide, as a hypoxia-selective cytotoxin or a radiosensitizer.

目的：检验和评价喹喔啉双氮氧化物衍生物QN-2013的选择性乏氧细胞毒性和放射增敏作用。

Our result showed the expressed HμOR desensitization could be induced by acute stimulation of μ-selective agonists DAGO and Ohm in a dose-dependent manner in G protein level.

在昆虫细胞表达的HμOR，用μ选择性激动剂DAGO和Ohm急性预处理，也同样导致HμOR的脱敏，并且随着预处理激动剂浓度增加，脱敏现象愈明显。

By allowing selective loss of data the buffer size is reduced.

允许有选择性的丢失数据，缓冲区大小得以减小。

Result In peanut seedling stage, spraying 900 ml/hm^2 quizalofop-P-ethyl EC on the grass weeds such as Barnyard grass, Green bristlegrass and Goosegrass had 100% control effect, but it had a poor control effect on some broad-leaved weeds. While, spraying 600 and 720 ml/hm^2 quizalofop-p-ethyllactuofen EC had remarkable effects not only on the grass weeds such as B. grass but also on the broad-leaved weeds such as Purslane, Cirsium setosum and Dayflower, with the control effect of more than 80%. Both of the 2 herbicides would not produce some slight contact drug spots on the part of peanut leaves and the drug spots on the leaves could be recovered in 1 to 2 weeks, which couldn't affect the growth and yield of the peanut in middle and late stage. So the 2 herbicides had good selectivity.

结果］在花生苗期喷施900 ml/hm^2精喹禾灵乳油对稗草、狗尾草、牛筋草等禾本科杂草具有100%的防效，但对一些阔叶杂草防效较差，而喷施600和720 ml/hm^2精喹乳氟禾乳油则除了对稗草等禾本科杂草有特效外，还对马齿苋、刺儿菜、鸭跖草等阔叶杂草具有显著的防效，均达80%以上。2种除草剂均对花生叶片局部产生一些轻度的接触性药斑，药斑在12周内可恢复，不影响花生的中后期生长及产量，具有很强的选择性。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷