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Therefore, N-phosphoamino acids could be used as models in the study of reactions of the phosphoryl group in the life process.First of all, a series of model N-phosphpoamino acids were synthesized. And their ester exchange reaction on the phosphorous center was studied by ~(31)P NMR, HPLC, ESI-MS and ESI-MS/MS.

首先,本论文从氨基酸角度研究磷化学,合成了一系列模型化合物N-磷酰化氨基酸,利用~(31)P NMR技术研究了磷上酯交换反应,发现α-氨基酸对于磷原子存在着手性选择现象,并且这种手性选择的差异随α-氨基酸种类的不同也有所区别。

Many researchers have devoted their work to the development of modal analysis extraction techniques in order to obtain more reliable identification of the modal parameters. There are many damping identification methods, but each one has different merit and defect. In order to find which one is the most reliable method with respect to certain characteristics, this thesis focus on how to identify structure's damping in time domain. The following works have been done in this thesis: 1. Investigate four modal analysis methods——the LSCE Method, the Ibrahim Time Domain Method, the Time Domain Collectivity Modal Parameter Method, the Rational Fraction Polynomial Method and improve on the ITD Method in arithmetic.

本文主要做了如下工作: 1、集中研究了四种常用的模态识别方法,即最小二乘复指数法、Ibrahim时域法、时域总体模态参数辨识法及有理分式多项式法,并对ITD法在算法上进行了一定的改进; 2、建立结构动力模型,利用这四种方法在具体条件下识别结构的阻尼,并绘制相应的图; 3、讨论不同的情况下,不同的识别方法对阻尼识别精确性的影响; 4、基于业已明确的阻尼识别的精确性,根据研究的需要,指出在实际的识别过程中,如何选择最佳的识别方法以及识别过程中需要注意的问题; 5、选择最为精确的阻尼识别方法,以导师李书进教授在日本京都大学防灾研究所强震反应实验室所做的一足尺木制结构房屋的动力测试实验为实测模型,识别其阻尼; 6、在本文的最后,尽作者所知,提出了一些需要改进的地方,并对未来的研究方向给出了作者的建议。

Unsteady state operation offers a new means of controlling total oxidation by segregating the hydrocarbon feedstock from oxygen.

丁烷选择氧化制顺酐是迄今唯一已经工业化的低碳烷烃选择氧化反应。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象,提取细菌基因组DNA,用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切,回收大小为1~5Kb的DNA片段,将其连接入原核表达载体pET-28a-c,最后转化宿主菌,结果成功地构建了基因表达文库,为后续的基因筛选工作奠定基础;另外,本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计,以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板,进行PCR扩增反应,结果表明成功地获得两个新基因:23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸),并进一步做了不同物种之间的分子系统发育分析,构建了基于23S rRNA基因的邻接法系统发育树,阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

The developer, trehalase amount, trehalose amount, reaction time and temperature, solvent were discussed. Validoxylamine test showed that the method of trehalase-inhibit in vitro is reliable for screening.

离体测试模型主要涉及溶剂的选择、显色剂选择、反应的温度和时间、海藻糖酶和海藻糖的用量等内容,所建立的离体测试模型用井岗霉素验证了该方法的可靠性。

Thus. Treatment of CR1R2=C=CH2 (R1 = R2 = Me; R1 = H, R2 = n-Bu; R2 = Ph; R2 = cyclohexyl; R2 = cyclopentyl, R2 = C6H5O, R2 = 2-IC6H4O, R2 = 4-IC6H4O) with bisdiborn in the presence of Pd2 (5 mol %) and 3-iodo-2-methyl-2-cyclohexen-1-one (5 mol %) in toluene at 80 oC gave products bearing both vinylic and allylic boron moieties in good to excellent yields.

但我们发现可以使用Pd金属错合物与有机碘化物共同催化下的系统,来进行此种加成反应。溶剂的选择以甲苯为最佳,Pd错合物以没有强配位基存在为最好的选择。

The design process is: to select the best process, drawing process maps, after the material of the process, heat is calculated to select the required specifications for production equipment models, a comprehensive natural conditions, transport, economic and other factors in selecting suitable areas The building addresses, format of the workshop construction, equipment to deal with layout, rendering plant layout, equipment flow chart, response equipment, such as design and graphics, the last of the water, electricity, gas and other public works to estimate the amount of ventilation, heating , environmental protection, fire prevention, safety and health content on to complete the preliminary design of the workshop.

本次设计的过程是:选择最佳工艺流程,绘制工艺流程图,之后对各工序物料、热量进行计算,选定所需生产设备规格型号,综合自然条件,交通,经济等个方面因素选择合适的建厂地址,对车间建筑形式,设备布置进行处理,绘制车间布置图、设备流程图,反应设备的设计与绘图等,最后对水、电、汽等公用工程用量进行估算,对通风、采暖、环保、防火、安全卫生等内容进行阐述,从而完成了车间的初步设计。

The design process is: to select the best process, drawing process maps, after the material of the process, heat is calculated to select the required specifications for production equipment models, a comprehensive natural conditions, transport, economic and other factors in selecting suitable areas The building addresses, format of the workshop construction, equipment to deal with layout, rendering plant layout, equipment flow chart, response equipment, such as design and graphics, the last of the water, electricity, gas and other public works to estimate the amount of ventilation, heating , environmental protection, fire prevention, safety and health content on to complete the preliminary design of the workshop.

本次设计の过程是:选择最佳工艺流程,绘制工艺流程图,之后对各工序物料、热量进行计算,选定所需生产设备规格型号,综合自然条件,交通,经济等个方面因素选择合适の建厂地址,对车间建筑形式,设备布置进行处理,绘制车间布置图、设备流程图,反应设备の设计与绘图等,最后对水、电、汽等公用工程用量进行估算,对通风、采暖、环保、防火、安全卫生等内容进行阐述,从而完成了车间の初步设计。

A different channel is selected from the plurality of multiplexed channels as the reselected RTrCH when a better candidate transport channel in the ON state becomes available, or when the monitored RTrCH reflects an OFF state.

当一较佳的候选载送频道的开状态存在时,或当该被监视的RTrCH反应一关状态时,不同的频道从该复数多路传输频道中被选择做为该再选择的RTrCH。

The choice, for example, of locust wood, a locally grown wood, completely riddled with all kinds of knots, blemishes, whorls and short-grained fibers, could not have been selected by Torres as a material ideally suited to produce the best possible tonal response.

选择, 例如,蝗虫木头(certonia 长角),当地增长木,完全地非难以各种各样的结,伤疤, whorls 和短成颗粒状的纤维,不能由Torres 选择了作为材料理想地适合导致最好音调的反应。

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In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国,慢性酒精中毒,肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题,你可以随时联系我。

Very pretty, but the airport looks more fascinating The other party wisecracked.

很漂亮,不过停机坪更迷人。那人俏皮地答道。