选择

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

According to the present invention, there is eliminated the need for an additional mode selection terminal to selectively access the internal memory or an external extended memory, and further that mode selection can be achieved by a signal applied to a terminal already being used for other purposes.

根据本发明，有淘汰，我们需要一个额外的模式选择端，以有选择性地获取内部存储器或外部内存扩展，并进一步指出模式选择，可以取得的一个信号，适用于终端已被用来作其他用途。

They will also have a choice of 7 exterior colours to choose from.

他们还将有选择的7外观颜色可供选择。

Don't externalize whatever indecision or inner conflict you have by doubling up on majors.

不要让你的优柔寡断或是内心的矛盾在选择专业上犯错误——选择两个专业。

They learn to fit eyeglasses and contacts prescribed by ophthalmologists and optometrists and to help customers choose eyeglass frames.

学生学习按照眼科医生和视光师的要求选择眼镜，以及帮助客户选择眼镜框等。

Du: Although you always talked about natural property of photography, I can feel your strong subjective opinions on eyepoint and frame. Why do you want such a view point? What do you stand for?

杜：你多次谈到摄影的自然性，但我感觉你选择的视点等，甚至包括画幅，都有你强烈的主观性在其中，那么你为什么要选择这么一种视角，你的态度和立场是什么？

Our buy program to Yahoo partner character fairness is reasonable, make them organic can select the tomorrow of the company, also offerred more alternative for content fabricant, advertiser and consumer at the same time.

我们的收购方案对于雅虎股东而言公平合理，让他们有机会选择公司的未来，同时也为内容制作者、广告客户和消费者提供了更多选择。

It is a good idea to have a fabricator in mind prior to selecting a slab.

选择一个好的生产商比选择大板来个重要得多。

It is the first important step in video analysis and will directly affects the effectiveness of indexing Shot boundary detection is one of our major research interests and we will tackle the following existing problems the ambiguity between gradual change and camera motion, the discontinuity during gradual change, false detection caused by illumination variation and flashlight, automatic threshold selection Firstly, we discuss shot boundary detection in non-compressed domain In chapter 2, we compare some of the commonly used detection methods which are based on frame difference and point out that single feature will not generate good results As a conclusion, we use fuzzy logic to combine multiple features Presently, most frame difference based shot boundary detection algorithms rely on threshold and hence the selection of such thresholds will greatly affect the performance of boundary detection We propose a membership function to define frame difference and calculate the membership with self adaptation according to the statistic distribution of frame differences to satisfy different type of video clips Experiments show that the proposed fuzzy shot boundary detection algorithm can be used with different video types and has a high detection precision and recall In chapter 3, we discuss model-based shot boundary detection algorithms regarding chromatic and spatial editing effects such as fade-in, fade-out, dissolve and wipe Various parameters are proposed to better describe the characteristics of each editing type.

镜头边界的检测是把视频自动地分割为一个个镜头，作为基本的索引单元，因此它是视频分析重要的第一步，直接影响到视频检索的成败。镜头边界的检测是本文研究的重点之一。目前镜头边界检测算法主要存在以下问题：渐变与镜头运动难以区别；渐变过程中的不连续与停顿、光照条件的变化及闪光灯等特殊情况会引起误检测；自动选择阈值比较困难等。本文首先针对非压缩域视频进行了镜头边界检测的研究。在第二章中我们采用了比较流行的基于帧间差的方法。在比较各种帧间差计算方法的基础上，指出使用单一的特征难以取得很好的检测效果，提出用模糊逻辑综合使用各种特征。目前大多数基于帧间差的镜头边界检测算法都采用阈值法进行镜头转换的判别。阈值选择的误差对检测性能有较大的影响，本文提出用隶属度函数定义帧间差较大、中等较大和较小等概念，并根据帧间差的统计分布自适应地确定隶属度，以适应不同类型的视频片断。实验结果表明这种基于糊逻辑的镜头边界检测算法可以适应不同的视频，并具有较高的检测精度和检出率。在第三章中采用基于模型的方法进行镜头渐变的检测，研究了淡入／淡出、慢转换和扫换的模型。

Basically what makes this a separate category from "All Missions", by using the Escape Unit in Anubistep and Fafnir's missions (2nd and 4th in respective order from Mission Select), and chose the "RETIRE" option,(I have mixed feelings on it, seeing as "Retiring" refers to the killing of Irregulars aka nutty-robo-baddies) doing this results in a 0'00 clear time, and will trigger the event where the Resistance Base is invaded (not assaulted with a big drill bomb robot, invaded)and after that it's just the three final stages.

基本上是什么使这是一个从"所有代表团的"单独一类，通过使用Anubistep和法夫尼尔的任务逃逸股（第二和选择各自的任务，以便第4次），并选择"退休"选项，（我有这么复杂的感情看到为"退"，是指非正规军又名做好1 0'00明确的时间这个结果坚果，机器人，坏人）杀害，并触发的事件中，抵抗侵略基地（不袭击大演习的炸弹机器人，入侵），之后这只是最后的三个阶段。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。