逆转录酶病毒

Measles virus ; S191; hemagglutinin gene ; reverse transcription polymerase chain reaction ; sequence analysis ; molecular epidemiology

麻疹病毒； S191； H基因；逆转录—聚合酶链反应；序列分析；分子流行病学

A 450 nucleotides fragment of the COOH-terminal of the nucleoprotein gene and 188 nucleotides fragment of the matrix were amplified by RT-PCR and subject to sequence.

用逆转录－聚合酶链反应扩增麻疹病毒分离株的核蛋白基因羧基端450个核苷酸片段和基质蛋白基因的188个核苷酸片段。

To construct a retroviral vector carrying immortalizing gene hTERT, screen gene puro, reporter gene EGFP and recombination site sequence LoxP for reversible cell immortalization. Linearized pBABE-puro was cut by restriction enzyme Nhe Ⅰ and ligated with loxP double strands to construct a vector pBABE-puro-lox.

目的 探讨以hTERT作为永生化基因、嘌呤霉素乙酰转移酶基因puro作为筛选基因、加强型绿色荧光蛋白EGFP作为报道基因、loxp作为重组位点构建可回复性永生化逆转录病毒载体，为建立可回复性永生化细胞株莫定基础。

A human transforming growth 〓 cDNA was inserte in reverse orientation into the retroviral vector, pLXSN. Restriction en zyme analysis was used to confirm the antisense orientation of 〓〓 in the pLXSN vector from individual transfomants.

将人的转化生长因子-〓cDNA（1467bp）反向插入逆转录病毒载体pLXSN，筛选转化菌，挑取抗性克隆，用限制性内切酶酶切鉴定重组子。

Because many antineoplastic drugs are also metabolized by the CYP system, coadministration with cART could result in either drug accumulation with increased toxicity, or decreased efficacy of one or both classes of drugs.

因为有许多抗肿瘤药物也是通过细胞色素酶系统来代谢的，当它们和抗逆转录病毒药物联合使用时会导致药物累积毒性增强，或者是某一类或几类药物疗效的下降。

Methods From January to March 2007, cases from both outbreaks and sporadic episodes of acute nonbacterial gastroenteritis were investigated in Beijing, and the fecal specimens of the patients were collected.

方法收集北京市2007年1—3月非细菌性胃肠炎暴发和散发病例，采集患者粪便标本，使用逆转录聚合酶链反应对粪便标本进行诺如病毒RNA检测，对RT—PCR阳性标本的PCR产物进行克隆测序。

Methods : The rotavirus RNA in the plasma samples and peripherial blood mononuclear cells of 30 patients with rotavirus diarrhea were measured by two kinds of reverse transcriptase polymerase chain reaction tests .

应用两种逆转录聚合酶链反应方法，对30例轮状病毒腹泻患儿进行外周血血浆和单个核细胞中的RV RNA检测，同时应用酶联免疫吸附法检测血浆IL-10水平，并与20例正常对照组进行比较。

Methods: Four hantavirus strains, which were positive by antigen immunofluorescence assay, were analyzed by reverse transcription polymerase chain reaction, nucleotide sequence and cell culture.

采用逆转录－聚合酶链反应、核苷酸序列测定和细胞培养方法对免疫荧光抗原阳性的4份鼠肺标本进行汉坦病毒分析。

We successfully constructed the recombinant retroviral vectors of NHE-1 specific hammerhead ribozymes and transfected them into the PASMCs in vitro.

1。本实验成功地构建了大鼠NHE-1特异性锤头型核酶的逆转录病毒载体，并成功转染入培养的PASMCs内。

Mothods: Cock embryo was used to isolate influenza viruses, and the virus titer was determined through hemagglutination test. The gene was amplified by RT-PCR technique from the isolated strain and sequenced, and the gene phylogenetic tree was analyzed.

采用鸡胚进行病毒分离，采用血凝试验初步确定病毒效价，用逆转录聚合酶链反应法扩增分离株的部分片段后进行克隆和测序，然后进行进化树分析。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。