逆转录酶病毒

Methods DNA clone of AD8 and NL43 was respectively transfected to skin by Gene Gun, The expression of virus in the epidermal cells was observed by flow-cytometry and fluoroscope.

以基因枪转染AD8及NL4 3分子克隆至皮肤，流式细胞仪和荧光显微镜观察病毒在表皮细胞中的表达，磁分离术分离CD80 +和CD80 -的游离细胞，进而以PCR和逆转录酶检测游走细胞中HIV对CD+ 4 T淋巴细胞的感染和表达。

Methods: The human MDK cDNA was amplied from the BGC823 cell line by high fidelity PCR.

扩增人MDK编码序列基因片段，经限制性内切酶酶切后将目的片段插入pLXSN逆转录病毒载体。

Methods:Using cell culture and immunohistochemical techniques,the peripheral blood specimens from 33 RA patients were designed to test the human T cell lymphotropic virus type Ⅰ related antigens,reverse transcriptase and spontaneous syncytium formation.

采用细胞培养、免疫组织化学技术等对33例RA患者的外周血样本进行人类嗜T淋巴细胞病毒Ⅰ型相关抗原、逆转录酶活性测定及观察自发性合胞体形成。

When HIV-1 in culture medium was exposed to a range of concentrations of urokinase, a dose-dependent inhibition of infectivity was observed in a variety of lymphoma and leukemia cell lines including MT4, CEM, H9, and peripheral blood mononuclear cells using assays for reverse transcriptase, P24 antigen and syncytia formation.

通过检测逆转录酶活力，P24抗原的表达和合胞体形成情况发现尿激酶可以抑制人体免疫缺损病毒对多种淋巴瘤和白血病细胞系，如MT4、CEM、H9和外周血单核细胞的侵染能力，并且这种抑制与尿激酶浓度呈剂量依赖关系。

Methods The hTERT promoter as E1A and E1Bp gene were amplified by PCR; the GFP gene and GM-CSF were also amplified by PCR, and the products were subcloned into Teasy plasmid in certain order to generate pSh-GFP-SV55K plasmid.

采用PCR的方法克隆腺病毒E1区基因(E1A， E1B55K)、EIB的启动子(E1Bp)以及人端粒酶逆转录酶亚基启动子，以及2个目的基因：人GM-CSF和GFP(Green Fluorescence Protein， GFP)。

This study paid more attention to the techniques of detecting Soybean mosaic virus in dry soybean seed and Bean common mosaic virus in dry bean seed by reverse transcription-polymerase chain reaction, combining with biological test and enzyme-linked immosorbent assay. The goal of the research is to establish a molecular detection procedure of SMV and BCMV from dry seed. The main results are following:⑴The effective techniques were made to extract total RNA of SMV from dry soybean seed and BCMV from dry bean seed.

本研究以大豆和菜豆种子中主要种传病毒大豆花叶病毒、菜豆普通花叶病毒作为检测对象，在生物学测定和酶联免疫吸附测定的基础上，研究干种子中两种病毒的逆转录-聚合酶链式反应检测方法，以建立一套快速、灵敏、专化性强的直接以干种子作为检测对象的分子检测技术体系，主要结果如下：⑴建立了从大豆和菜豆干种子中提取SMV、BCMV总RNA的有效方法。

Borna disease virus ; Reverse transcription-polymerase chain reaction ; Receptor ; Serotonin ; Dopamine ; Transcription ; genetic

Borna病病毒；逆转录聚合酶链反应；受体；血清素；多巴胺；转录；遗传

Objective This study aimed to establish a fast and accurate quantitative method to detect Borna disease virus ribonucleic acid using fluorescence quantitative nested reverse transcriptase polymerase chain reaction.

目的：本研究拟建立博尔纳病病毒荧光定量套式逆转录酶聚合酶链反应检测方法，为快速、准确地定量检测 BDV 奠定基础。

Objective This study aimed to establish a fast and accuratequantitative method to detect Borna disease virus ribonucleic acid using fluorescence quantitative nested reverse transcriptasepolymerase chain reaction.

目的：本研究拟建立博尔纳病病毒荧光定量套式逆转录酶聚合酶链反应检测方法，为快速、准确地定量检测 BDV奠定基础。

The total RNA of viruses isolated was extracted from the Hantavirus-infected Vero-E6 cells and the S-segment cDNA of Hantavirus was obtained by RT-PCR. This gene fragment was then cloned into plasmid vector pUCm-T and sequenced afterwards by using the DNA STAR software for comparison.

提取病毒总RNA，应用逆转录聚合酶链反应扩增病毒S基因全片段，克隆入质粒载体，进行核苷酸序列测定及分析，应用DNA STAR软件分析比较，确定病毒型别。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。