逆转

At the South Edge of the Tengger Desert, the sampled sites of mobile sanddune, semi-fixed sand-dune and fixed sanddune were selected as the different stages of the reversion process of desertification with the method of substituting time with space, and topsoil mineral elements of the decertified land were studied reversion process of desertification.

以腾格里沙漠南缘沙漠化土地为研究对象，应用时空替代法选择流动沙丘、半固定沙丘、固定沙丘的沙漠化逆转序列样地，研究了沙漠化逆转过程土壤矿质元素的变化特征。

Methods］ MTT assay was employed to test the lethal concentration 50 (IC50) of sensitive germline (SK-OV-3) and ovarian cancer cell subline induced by ADR (SK-OV-3/adr) treated by 4 drugs, resistance index and reverse multiple were calculated. The changes of drug content were observed by fluorescence microscope, the intracellular accumulation of ADR was evaluated by fluorospectrophotometry, the changes of mdr 1/P-gp resistance were observed respectively by RT-PCR and Western blot.

方法］MTT法检测药物对敏感细胞株（SK-OV-3）和诱导的耐药细胞株（SK-OV-3/adr）的半数致死浓度（IC50），计算耐药指数和加逆转剂后的逆转倍数，荧光显微镜观察不同时间细胞内的药物含量变化，荧光分光光度法测定细胞内ADR的含量，RT-PCR和Western blot法检测细胞耐药前后mdr1/P-gp的变化。

AIM: To isolate bioactive secondary metabolites from the fruits of Schisandra rebriflora Rehd. et. Wils and study their effects on HIV-1 infectivity. METHODS: Inhibition of syncytia formation, HIV-1 reverse transcriptase and protease, protection of HIV-1 infected cells, level of HIV-1 p24 antigen,blockage of fusion were detected. RESULTS: A lignan compound, named rubrifloralignan A, was first isolated as a natural product with modest inhibition effects on syncytium formation and HIV- 1 replication and could protect HIV-1 infected cells with weak inhibition effects on fusion and RT, but it showed no effects on the replication of HIV-1 chronic H9 and PR. CONCLUSION: Rubrifloralignan A is an efficient anti-HIV-1 product with effect on early stage of HIV-1 replication.

目的：从红花五味子果实中寻找活性代谢产物，研究其抗HIV—1活性和作用机制方法：通过合胞体抑制、HIV-1感染细胞保护、HIV—1p24抗原测定、融合阻断、逆转录酶和蛋白酶活性分析等实验，检测分离化合物的抗HIV—1活性并探讨其作用机制一结果：红花五味子甲素能够抑制病毒诱导的合胞体形成，保护病毒感染细胞，抑制病毒在细胞内的复制对HIV-1感染细胞与正常CD4^＋细胞间的融合，逆转录酶活性有一定的抑制作用但是红花五味子甲素不抑制病毒在慢性感染细胞中的复制，也不抑制蛋白酶的活性、结论：首次从天然产物中分离得到红花五味子甲素，它对HIV—1病毒复制早期具有抑制作用

For most viruses,there is aneed for antimicrobials that target unique viral molecular properties.Acycloviris one such drug.It is activated into ahuman herpesvirusDNA polymerase inhibitor exclusively by HHV kinases and,thus,does not suppress other viruses.Here,we show that ACV suppresses HIV-1in HHV-coinfected human tissues,but not in HHV-free tissue or cell cultures.However,addition of HHV-6-infected cells renders these cultures sensitive to anti-HIV ACV activity.We hypothesized that such HIV suppression requires ACV phosphorylation by HHV kinases.Indeed,an ACV monophosphorylated prodrug bypasses the HHV requirement for HIV suppression.Furthermore,phosphorylated ACV directly inhibits HIV-1reverse transcriptase,terminating DNA chain elongation,and can trap RT at the termination site.These data suggest that ACV anti-HIV-1activity may contribute to the response of HIV/HHV-coinfected patients to ACV treatment and could guide strategies for the development of new HIV-1RT inhibitors.

对大多数病毒而言，都需要有针对其分子特性的靶向杀毒剂阿昔洛韦就是这样一种靶向药物在人疱疹病毒酶的特定作用下，阿昔洛韦被激活成为人疱疹病毒DNA聚合酶抑制剂，因此不能再抑制其它的病毒我们的研究发现阿昔洛韦在共感染人疱疹病毒的组织中可以抑制HIV-1，但在无人疱疹病毒感染的组织或细胞中无此作用然而，加入人疱疹病毒-6感染的细胞却使得其对抗HIV的阿昔洛韦变得敏感我们推测这种抑制作用依赖于人疱疹病毒酶导致的阿昔洛韦磷酸化实际上，单磷酸化的阿昔洛韦前体药物无需人疱疹病毒的参与即可抑制HIV此外，磷酸化的阿昔洛韦能直接抑制HIV-1逆转录酶，将其阻止在终止位点，从而终止DNA链的延长这些结果提示阿昔洛韦的抗HIV-1活性决定了艾滋病病毒/人疱疹病毒共感染的患者对阿昔洛韦的治疗反应，也有助于开发新的HIV-1逆转录酶抑制剂

AIM: To study the multidrug resistancereversing effect of Chinese drug Thallus Laminariae PE on human endometrial cancer cell line BMDC1 in vitro and its underlying mechanism.

目的：研究昆布提取物在体外对耐药细胞BMDC1的逆转作用，探讨其逆转肿瘤多药耐药的机制。

Objective To investigate the curative effect of left ventricular hypertrophy with uretic indapamide, Methods 96 patients wiht LVH were divided into two groups, 50 patients in A group were treated with indapamide 2,5～5mg/d, and 46 patients in B group with nifedipine 20～60mg/d for 12 months, Results Blood pressure、interventricular septum thickness、left ventricular posterior wall thickness、left ventricular mass index and left ventricular ejection fraction in all cases were improved after treatment, A group was better than B in LVEF, Conclusion Both indapamide and nifedipine can regress LVH, and improve left heart function

目的 观察新型噻嗪类利尿剂吲达帕胺逆转左心室肥厚的效果。方法 96例高血压LVH患者，分别给予吲达帕胺(A组，50例)2，5～5mg/d和硝苯地平控释片(B 组，46例)20～60mg/d持续12个月治疗，观察治疗前后血压及左室结构变化。结果两组治疗前后血压、室间隔厚度、左心室后壁厚度、心室重量指数和左心室射血分数均有改善(P＜0，05～0，01)，A组在改善LVEF方面优于B组(P＜0，05)。结论吲达帕胺具有与硝苯地平类似的逆转LVH作用，有明显改善左心功能作用。

Hydrocortisone is widely used in patients with septic shock even though a survival benefit has been reported only in patients who remained hypotensive after fluid and vasopressor resuscitation and whose plasma cortisol levels did not rise appropriately after the administration of corticotropin.

1月10日《新英格兰医学杂志》报道，Hadassah希伯来大学医学中心等地的研究人员证实，无论是对所有的脓毒性休克患者还是只针对那些对促肾上腺皮质激素无反应的患者，氢化可的松都不能改善生存率或逆转休克症状；但是，对于已经逆转了休克进程的患者来说，氢化可的松可以加快休克的恢复。

Objective To construct retrovirus vectors carrying IL17 or siRNAIL17 genes with Thy1.1 gene and determine the infected ability of the retrovirus vectors to diabetogenic BDC2.5 T cells.

目的 构建含有Thy1.1细胞表面抗原(Thy1.1 cell surface antigen，Thy1.1)基因的携带IL17或siRNAIL17基因的逆转录病毒载体，并观察逆转录病毒对致糖尿病性BDC2.5 T细胞的转染能力。

Methods The IL17 cDNA and Thy1.1 fulllength cDNA were subcloned into MIT(MSCVIRESThy1.1) retrovirus vector, and the siRNAIL17, U6 promoter and Thy1.1 fulllength cDNA were also inserted into retrovirus vector of pMNDBANSHEE.The recombined vectors were transfected 293 packaging cells by DNA calcium phosphate coprecipitation. Virus supernatant which infected preactivated spleen cells from NOD/BDC mice was collected. After incubation, the IL17 expression in diabetogenic T cells was detected.

利用基因工程和细胞克隆技术分别将IL17的cDNA插入MSCVIRESThy1.1逆转录病毒载体，将Thy1.1、U6增强子(U6 promoter)基因和IL17的siRNA cDNA插入逆转录病毒载体pMNDBANSHEE，采用磷酸钙沉淀法将重组载体转染293包装细胞；收集病毒上清，转染预先活化的NOD/BDC小鼠脾细胞，测定致糖尿病性T细胞的IL17和siRNAIL17的表达。

Objective To investigate the functional mechanism of Chinese drug disodium cantharidinate reversing multidrug resistance of tumor cells.

目的 观察斑蝥酸钠对多药耐药的白血病细胞(K562/AO2)细胞是否有逆转作用，并初步探讨其逆转机制。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。