逆转

Objective: To construct expression vector of hTERT-hIL-18 fusion gene in eukaryotic cells and to study its biological function.

目的：以基因重组技术在真核细胞中表达人白细胞介素18(hIL-18)和人端粒酶逆转录酶融合基因，并观察其生物学功能。

Methods Semi -quantitative reverse transeriptase - polymerase chain reaction was used to examine the expression of BCRP gene in 62 cases of malignant hematologic diseases, BCRP/β2 - MG ratio was defined as BCRP expressible level.

方法应用半定量逆转录-聚合酶链反应，β2微球蛋白基因作内参照，以BCRP／β2-MG的比值作为BCRP基因的相对表达水平，检测62例恶性血液病BCRP基因的表达。

METHODS: Liver fibrosis models were established in 24 SD rats by intragastric administration (7 g/kg) of alcohol (56°, 560 mL/L) every morning, and 10 normal rats served as controls. The rats in model group were killed by exsanguination from femoral vein at 4, 12 and 24 wk. Pathological changes of liver tissues in rats were identified by Masson staining, and the expression of VEGF mRNA was detected by semiquantitative reverse transcription-polymerase chain reaction.

56度的白酒(560 mL/L)平均以7 g/kg的剂量每日早晨灌胃一次制备肝纤维化模型，灌胃4 wk、12 wk及24 wk采用股静脉放血法分别处死大鼠，观察肝脏病理变化并采用半定量逆转录聚合酶链反应检测VEGF mRNA的表达。

From traditionary nucleus pulposus exsection to spinal fusion,endoscope and minimally invasive techniques,and prosthetic disc nucleus and artificial disc replacement in recent years, however,they relieved clinical complaint temporary by means of the destruction of structure and function of lumbar intervertebral disc.

从传统的髓核摘除术到脊柱融合术、内窥镜和微创技术及近年开展的人工髓核和椎间盘置换术，但它们都是以破坏椎间盘结构和牺牲生物力学功能为代价的，不能阻止和逆转椎间盘退变，因此椎间盘退变的基因治疗和组织工程成为研究的热门课题。

At a certain moment this stream speeds up to such an extent that the field of the magnet reverses completely.

在某一时刻本流加速到这样的程度领域的磁铁完全逆转。

The company President Nicolas Sarkozy is keeping these days is unlikely to help reverse his flaccid approval rating.

这些天，即使尼古拉·萨科齐总统陪在布什身边也不太可能逆转他虚弱的支持率。

Objective: To package five recombinant retroviral vectors containing the enhanced green fluorescent protein gene and screen for the best hepatocytes-targeting retroviral vector.

目的：包装5种携带增强型绿色荧光蛋白基因的重组逆转录病毒，筛选肝细胞靶向性好的病毒载体。

Methods DNA clone of AD8 and NL43 was respectively transfected to skin by Gene Gun, The expression of virus in the epidermal cells was observed by flow-cytometry and fluoroscope.

以基因枪转染AD8及NL4 3分子克隆至皮肤，流式细胞仪和荧光显微镜观察病毒在表皮细胞中的表达，磁分离术分离CD80 +和CD80 -的游离细胞，进而以PCR和逆转录酶检测游走细胞中HIV对CD+ 4 T淋巴细胞的感染和表达。

Plus, studies show this green fountain of youth may help reverse some signs of aging.

此外，研究表明这种绿色的"青春原料"可逆转某些细胞老化。

Some might argue against these countries' choosing to allow free capital mobility on the grounds that rapid reversals in international capital flows have induced balance-of-payments crises and difficult domestic adjustments for them in the past.

有些人可能反对这些国家实行自由的资本流动，其理由是，国际资本流动的急剧逆转过去已经诱发过国际收支危机，迫使国内进行艰难的调整。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。