逆行

Methods: The model of multi-infarction dementia was made by means of retrograde catheterization infusing embolus, which was made of airdry sterilized blood clot of homogenic rats, to arteriae carotis externa.

取同种大鼠无菌自然干燥血凝块研碎，于颈外动脉处逆行插管注入栓子制作多发性梗死性痴呆动物模型。

However, good results and acceptable morbidities can be achieved under profound hypothermic circulatory arrest with retrograde perfusion.

然而，以深度低温循环中止合并逆行性灌注的方式进行手术可得不错的手术结果与可接受的手术并发症。

Methods: The experiment was performed in 6 adult rabbits with the method of retrograde axonal transportation of horseradish peroxidase, by which HRP was smeared on the proximal cut end of the left ilioinguinal nerves.

成年家兔6只，采用HRP逆行追踪方法，在左侧髂腹股沟神经近侧断端涂抹HRP。

Methods: The experiment was performed in 6 adult rabbits with the method of retrograde axonal transportation of horse radish peroxidase, by which HRP was smeared on the proximal cut end of the left ilioinguinal nerves.

成年家兔6只，采用HRP逆行追踪方法，在左侧髂腹股沟神经近侧断端涂抹HRP。

Use in a rolling way, stay for a time at inflammative place or problematic skin to increase the effect.

口周部：由嘴角部开始，沿法令门做提升动作，然后向前轻轻滑动，终止在鼻根部，接下来，从鼻翼开始，沿面颊到耳前，冰球要顺着皮肤和肌肉的纹路方向轻轻滑动，不可逆行，在有问题的皮肤处，可做短暂停留，以安抚过敏皮肤，另一只手可做柔和的安抚，动作重复4、5遍。

Cerebral perfusion was restored through innominate artery cannulation after excluding error of measurement.

继续血流降温到深低温进行手术。2例患者进行了上腔静脉逆行灌注脑保护。

Objective:To evaluate the efficacy of retrograde intramedullary nail and plate in the treatment of femoral intercondylar fracture.

目的 ：评价逆行交锁髓内钉与钢板治疗股骨髁部骨折的疗效。

Objective: To summarize the clinical experience of retrograde intramedullary supracondylar nail in the treatment of type C femoral intercondylar fracture.

目的 ：对应用髁上逆行交锁髓内钉治疗股骨髁C型骨折的临床经验进行总结。

It was achieved by intradural microanastomosis of the L6 ventral root to S2 ventral root. After axonal regeneration, the "patellar ligament-spinal cord center-bladder" reflex pathway was reestablished. The function of the reflex arc was observed by nerve electrophysiological experiment, detrusor electromyography experiment, urodynamic testing and HRP retrograde tracing before and in 8 months after the spinal cord was destroyed horizontally between S1 and S4 level. There was one case of conical SCI patient who had L1 fracture and paraplegia was received intradural microanastomosis of the L6 ventral root to S2 ventral root after Li fracture and paraplegia.

将Beagle犬L6前根近端与S2前根远端在硬膜内显微吻合，经一段时间的轴突再生后，建立&膝腱－脊髓中枢－膀胱&人工反射弧，术后8个月，在破坏S1~S4脊髓节段前后分别进行神经电生理、膀胱肌电图及尿流动力学和辣根过氧化物酶逆行示踪等检查；对1例L1压缩性骨折伴截瘫患者行右侧T11与52前根经腓肠神经移植硬膜内吻合术。

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。