适切的

At present, JACKROM a lot of blocking and pressure concavo-convex or offline production, once employees on version of the business card printing and membership card making and putting it all, the company will from the complete article on the web flexo business card printing and membership card production, bronzing, pressure concavo-convex, die-cutting of considerable benefit.

目前，JACKROM很多烫金和压凹凸还是在离线生产，一旦员工对柔版的制卡和会员卡制作适性全部掌握后，公司将从完整的联线柔版制卡和会员卡制作、烫金、压凹凸、模切过程中获得相当大的效益。

Zymologic analysis showed that the molecular weight of this β-mannanase was approximately 47 kD. The enzyme was stable when pH ranged from 5.0 to 6.5 and could be activated by Na+ and Ba2+. With an optimal temperature of 50°C. Action mode analysis of TLC re-vealed that the enzyme belonged to the endo-β-mannanase family.

酶学性质分析：该酶分子量约为47 kD，酶的最适反应温度为50°C，在pH 5.0~6.5之间该酶的稳定性较好； Na+和Ba2+对该酶有激活作用，用TLC对酶产物分析，表明该酶为内切β-甘露聚糖酶。

Elaborated the punching components formation principle, the basic dies structure and the rate process and the principle of design; and designed some conventional punching die:the die for big diameter three direction pipe which solved the problom of traditional machining,the drawing and punching compound die with float punch-matrix,the drawing and cutting compound dies with unaltered press,the compound die for the back bowl of the noise keeper,the design of the compound die which could produce two workpieces in one punching,the bending die for the ring shape part ,the bending die which used the gemel ,automate loading die for cutting, the drawing,punching and burring compound dies with sliding automated loading,the punching die for the long pipe with two row of hole,the drawing die for the square box shape workpiece and the burring die for the box shape workpiece.

原文对于寒冲压技术的合种、特征及开展圆背做砬简单概述；阐述了冲压整机的构成本理、基础模具构造与活动进程及其设计本理；对典范的冲压件模具进佳的了设计：大曲径三通管冲压复合模设计掀世了大直径三通管的减工困难、带无沉动凸凸模的拉伸冲孔复合模设计、适力压边式落料拉伸切边复合模设计、长音器后挖碗落料、拉深、冲孔、翻边复合模设计、一模二件落料拉伸冲孔翻边复合模设计、环形弯曲件的模具设计、铰链舒方成形工艺及模具设计、半自动送料切角安装设计、涩板式送料拉深、冲孔、翻边模设计完成自动送料、长管少孔冲模设计、带凸缘台阶方盒形整件拉深模战盒形件正壁小孔翻孔模。

Elaborated the punching components formation principle, the basic dies structure and the rate process and the principle of design; and designed some conventional punching die:the die for big diameter three direction pipe which solved the problom of traditional machining,the drawing and punching compound die with float punch-matrix,the drawing and cutting compound dies with unaltered press,the compound die for the back bowl of the noise keeper,the design of the compound die which could produce two workpieces in one punching,the bending die for the ring shape part ,the bending die which used the gemel ,automate loading die for cutting, the drawing,punching and burring compound dies with sliding automated loading,the punching die for the long pipe with two row of hole,the drawing die for the square box shape workpiece and the burring die for the box shape workpiece.

论述了冲压零件的形成原理、基本模具结构与运动过程及其设计原理；对典型的冲压件模具进行了设计：大直径三通管冲压复合模设计解决了大直径三通管的加工难题、带有浮动凸凹模的拉伸冲孔复合模设计、适力压边式落料拉伸切边复合模设计、消音器后补碗落料、拉深、冲孔、翻边复合模设计、一模两件落料拉伸冲孔翻边复合模设计、环形弯曲件的模具设计、铰链卷圆成形工艺及模具设计、半自动送料切角装置设计、滑板式送料拉深、冲孔、翻边模设计实现自动送料、长管多孔冲模设计、带凸缘台阶方盒形零件拉深模和盒形件侧壁小孔翻孔模。

Elaborated the punching components formation principle, the basic dies structure and the rate process and the principle of design; and designed some conventional punching die:the die for big diameter three direction pipe which solved the problom of traditional machining,the drawing and punching compound die with float punch-matrix,代写医学论文,the drawing and cutting compound dies with unaltered press,the compound die for the back bowl of the noise keeper,the design of the compound die which could produce two workpieces in one punching,the bending die for the ring shape part ,the bending die which used the gemel ,automate loading die for cutting, the drawing,punching and burring compound dies with sliding automated loading,the punching die for the long pipe with two row of hole,the drawing die for the square box shape workpiece and the burring die for the box shape workpiece.

本文对于冷冲压技术的分类、特点及发展方向作了简略概述；论述了冲压零件的形成道理、基本生产模型结构与运动历程及其设计道理；对于典型的冲压件生产模型进行了设计：大直径三通管冲压复合模设计盖世了大直径三通管的加工难题、带有浮动凸凹模的拉伸冲孔复合模设计、适力压边式落料拉伸切边复合模设计、消音器后补碗落料、拉深、冲孔、翻边复合模设计、一模两件落料拉伸冲孔翻边复合模设计、环形屈曲件的生产模型设计、铰链卷圆成形工艺及生产模型设计、半自动送料切角装置设计、滑板式送料拉深、冲孔、翻边模设计实现自动送料、长管多孔冲模设计、带凸缘台阶方盒形零件拉深模以及盒形件侧壁孔眼翻孔模。

Elaborated the punching components formation principle the basic dies structure and the rate process and the principle of design; and designed some conventional punching die:the die for big diameter three direction pipe which solved the problom of traditional machiningthe drawing and punching compound die with float punch-matrixthe drawing and cutting compound dies with unaltered pressthe compound die for the back bowl of the noise keeperthe design of the compound die which could produce two workpieces in one punchingthe bending die for the ring shape part the bending die which used the gemel automate loading die for cutting the drawingpunching and burring compound dies with sliding automated loadingthe punching die for the long pipe with two row of holethe drawing die for the square box shape workpiece and the burring die for the box shape workpiece.

本文对冷冲压技术的分类、特点及发展方向作了简略概述；论述了冲压零件的形成原理、基本模具结构与运动过程及其设计原理；对典型的冲压件模具进行了设计：大直径三通管冲压复合模设计解决了大直径三通管的加工难题、带有浮动凸凹模的拉伸冲孔复合模设计、适力压边式落料拉伸切边复合模设计、消音器后补碗落料、拉深、冲孔、翻边复合模设计、一模两件落料拉伸冲孔翻边复合模设计、环形弯曲件的模具设计、铰链卷圆成形工艺及模具设计、半自动送料切角装置设计、滑板式送料拉深、冲孔、翻边模设计实现自动送料、长管多孔冲模设计、带凸缘台阶方盒形零件拉深模和盒形件侧壁小孔翻孔模。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失