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Methods The hairpin sequences of siRNAs targeting VR1 gene of rat were designed, and two pairs of oligonucleotide sequence were synthesized. The annealed oligonucleotide fragments were cloned into linearized pRNAT-U6.2/Lenti expression vector and identified by PCR and DNA sequencing.

设计靶向大鼠VR1基因的发夹状siRNA,合成两对互补的寡核苷酸序列,退火后克隆到酶切的pRNAT-U6.2/Lenti siRNA载体,通过PCR和DNA测序鉴定重组质粒。

On the basis, this paper has further made a research on the high temperature graphitising annealing, the microstructure, as well as the mechanical properties of the high manganese malleable cast iron.

在此基础上,对这种高锰可锻铸铁的高温石墨化退火、显微组织及力学性能进行了初步的研究。

Since the Manganin pressure gauge is created, we can make some tests on it. At first, we observe the variation of resistance under some processing conditions.

本论文研究的目的在於改善高压下之 Manganin 线的电阻之安定性,实验条件上针对绝缘材料、构造、退火方法等参数探讨Manganin 线的电阻之安定性。

Precision of the pressure gauge depends not only on the material properties of the manganin, but also on the insulation of the wire, nealing of coil, seasoning procedures of the wires.

压力量测值的精确与否除与构成线圈的 Manganin 细线本身的材质有密切的关系之外,制造时对线材所做的绝缘、退火、季化等前处理和关於卷绕方式、框架材质等等也有直接的关系。

Compared with the annealing treatment, laser irradiation is a fast and effective way to tailor CMR manganites for industrial applications.

根据对传统退火效应的分析和理论计算,认为激光辐照导致的表面微结构的变化以及薄膜的氧含量和均匀性的提高对LBMO薄膜的磁电性能的改善与优化密切相关。

Then, the samples were annealed at high temperature to investigate the thermal stability of the copper metallized devices.

接著,试片放入空气中高温退火以研究元件的热稳定性。

Based on measured data,we advance the viewpoint that the interface trap has current conductive property besides being charged up, and the conduction is assumed to be the generation or recombination current caused by new interface traps, which can not be simply identified quantitatively from the I - V curve.

根据所测数据,明确提出了有自退火效应样品的新增界面陷阱除了电荷效应外还具有传导电流能力的观点,初步认为该电流是表面费米能级和陷阱能级相互作用导致的产生复合电流,该电流不能简单地从 I - V 曲线上定量分

Quasi-1D features in carrier transport, recombination and emission, et al., were studied in the V-grooved QWR samples modified by selective ion-implantation and rapidly thermal annealing, by means of Micro-photoluminescence and magneto-transport measurement.

利用显微光致发光光谱研究经选择性离子注入和快速热退火后的单根GaAs/AlGaAs量子线结构体系中的载流子在输运、复合发光等方面的一维特性。

Successfully, we improve both the electrical performance and reliability by the nitride capping and post-annealing process below 200℃.

结果显示,我们在低於200℃之下所作的氮化矽覆盖步骤以及退火处理,同样成功地改善元件在挠曲下的特性表现。

The nitrogen-doped Czochralski and nitrogen-free Czochralaki silicon that are heavily doped with antimony are inveatigated through different heat treatments.

通过在不同条件下退火,研究氮杂质对重掺锑矽中氧沉淀的影响。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。