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When the temperature for denaturalization decreased to 85℃ or below, and that for annealing increased to 72℃, no visual PCR product was observed on agarose gel electrophoretic profile.

当变性温度降低至85℃及85℃以下,退火温度升高至72℃时,琼脂糖凝胶电泳肉眼观察不到扩增产物。

The following PCR procedure was selected: 47 circles in the whole amplification: Denaturalize in 94 ℃ for 40s, melt in 36℃ for 50s, extend in 72℃ for 90s.

用下列PCR反应程序:反应过程包括47个循环:94℃变性40s,36℃退火50s,72℃延伸90s。

METHODS: The procedure conditions of PRC: 40 cycles of denaturation temperature from 88 ℃ to 97 ℃ for 5 s and 40 s respectively, and the temperatures of anneal and extension was 60 ℃ for 40 s.

聚合酶链反应运行程序条件:变性温度分别为88~97 ℃,变性时间分别为5 s和40 s,退火和延伸60 ℃,40 s,循环数40。

PCR is one of the common techniques in molecular biology, which can amplify nucleic acids through the cycle of denaturation, annealing and extension.

PCR是最常用的分子生物学技术之一,通过变性、退火和延伸的循环来完成核酸分子的大量扩增。

Eachcycle consisted of 45 sec. at 94℃ to denature, 45 sec. at 36℃ for theannealing primer, and 120 sec. at 72℃ for extension. The otherreactions were completed under 10 min. of extension at 72℃.

扩增反应在热循环仪上完成,程序设置为94℃变性处理45秒钟,36℃引物退火45秒种,72℃链延伸2分钟,依此作45个循环,然后再在72℃条件下使被扩增的链延坤10分钟完成全部反应。

The results showed the obtained parameters by SAA were optimal parameters.(2)The new algorithm for frequency derangement of wavelet packet transform is presented.

结果表明,与其它参数的去噪结果相比,模拟退火算法寻找到的这些参数即是最优的参数。

The normal hybrid genetic algorithms include genetic algorithm combined with steepest descent method and simulated annealing genetic algorithm.

常见的混合遗传算法有遗传算法与最速下降法相结合、遗传算法与模拟退火法相结合。

A practical cooling regime was established then, and a network with better performances was obtained by combining simulated annealing algorithm with gradient descent method.

将模拟退火算法与传统的梯度下降法结合,用于人工神经网络的优化与预测,得到了预测效果等与迭代时间性能更好的神经网络。

Finally, the comprehensive properties of the product were investigated, such as annealing detergence, wettability, stability and lubricating property for rolling experiment.

最后通过退火清净性、润湿性能、储存稳定性及轧机实验,对产品综合性能进行了考察与评价。

It employs a shrink training algorithm base on the deterministic annealing global optimization technique. This training algorithm adopts a maximum a posteriori optimization criterion.

它采用了一种基于确定性退火全局优化技术的收缩训练算法,这种算法以最大后验概率为优化标准。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。