连续法

Methods①The minimum inhibitory concentration and the minimum fungicidal concentration of citral against C.

方法①参照NCCLS M27A方案中的微量液基稀释法测定柠檬醛和氟康唑对原始白念珠菌的最低抑菌浓度和最低杀菌浓度；②采用多步诱导法，观察在含梯度浓度(1～50MIC)柠檬醛培养基上连续转种的白念珠菌MIC的变化情况，以氟康唑作为阳性对照，同时设空白对照和溶媒对照；③将敏感性下降的诱导株在无药培养基上连续转种10次后，检测其MIC值；④测定柠檬醛对氟康唑敏感性下降诱导株的MIC值。

For more upgrade aluminium oxide yield, optimize indices, energy conservation and save cost, it is necessity to desilicify steps reconstruction.In order to overcome the shortcoming of the desilication used in Shangdong Alumina Corpotation,three technology such as indirect heating, continious desilication,deep desilication by separation of the sodium-silicon residue.

本文通过对理论界关于烧结法生产氧化铝脱硅原理的研究，结合国内主要氧化铝生产厂家脱硅实践方面的经验，针对山东铝业公司烧结法氧化铝生产脱硅工序的现状，运用间接加热连续脱硅、钠硅渣分离深度脱硅和HCAC法高效脱硅三项科研成果，对原有的三次脱硅工艺改为二次脱硅工艺。

It is proved that the method using the BEM to calculate the load distributions on the contact lines of involute gear pair, in which the applied load is taken in the form of a concentrated one at a single node, is not suitable. A method in which the tractions of the BEM are made to imitate the Hertzian pressure of the gear using 8 nodes isoparametric elements is introduced.

本文解决了边界元法在空间啮合副计算中存在的系数矩阵的存储，奇异单元积分的计算、表面不连续点处面力一点多值和求解未知量的线性方程组矩阵元素的计算等问题，编制了比不采用上述处理方法的一般边界单元法节省近一半内存和计算时间的适合空间啮合副的三维边界元法计算程序。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Methods The survey was conducted among 252 medicos by accounting method, the 24 h dietary recalling method and weighing method for 5 days.

采用记帐法结合24 h膳食回顾法、称重法，对252名医学生进行连续5 d的膳食调查。

Met hods totally 252 medicos were investigated by accounting method, the 24 h dietary recalling method and weighing method.

采用记帐法结合24 h膳食回顾法、称重法对252名医学生进行连续5 d的膳食调查。

In addition, our cell can continuously work for up to 10 days, which also outlives that of the thermal beam cell technique.

而这与美国国家标准与技术研究院类似诉求的原子束腔法比较起来，我们的方法，光谱讯号与光功率比高於平行系列热原子束法，蒸汽室连续运转的时间可以达10天以上不用破真空补充钙，远比原子束腔法的13个小时长。

At first, I developed the principle and restrictive factor to optimize the investment structure of regional industry; Secondly, I applied the Grey Theory to research the relationship between structure of investment and structure of industry, the relationship between structure of industry and economy of region.; Thirdly, Three methods, including AHP, Principal Component Analysis and Relative Potential were exerted to research the order of investment afterward, I developed qualitative analysis to the third industry, and put forward suggest to its progress direction; Fourthly, Using economics and operational researchs thoughtway for reference, I upbuilt the dynamic model of investment portion model.

首先，提出了优化区域产业投资结构的原则和制约因素；其次，运用灰色系统理论对区域的产业结构与整体经济、投资结构与产业结构的关系进行论证分析；第三，建立了区域产业投资结构评价指标体系，并运用AHP法、主成份分析法和相对势法对河北省第一、第二产业投资排序进行了研究，然后，在充分利用现有数据的前提下对第三产业进行了定性的分析，并对第三产业的发展方向提出了建议；第四，借鉴了经济学、管理学、运筹学等一些理论的思想方法，建立了区域产业连续投资分配比例模型；最后，根据河北省情，结合中国进入WTO的过渡期市场环境，提出了一些政策建议，以达到实现河北省产业投资结构优化的目的。

Simulation results show that the relative-effective error of bridge structure parameter identification is less than 10%, the application of Levenberg-Marquardt algorithm is feasible, its quantitative message can be put forward for bridge structure condition assessment.

对一连续粱的数值模拟计算表明，在Gauss-Newton法迭代发散的情况下，Levenberg—Marquardt法的识别结果相对误差在10％左右，Levenberg—Marquardt法基本能实现对真实结构参数的识别，为结构进一步的状态评估提供了结构模型最基本的量化信息。

This simulation system will help to improve innovation design level of high-speed cultivation machine such as high-speed plow and high-speed rotary tiller. SPH; back-rotary tilling ; simulation ; soil-tool interaction ; high-speed cutting

因为牵涉到多相、松散和物性分散的耕作土壤在工作部件高速作用下的破坏过程以及破坏后土壤运动状态的描述，用连续体力学理论及其数值解法(有限单元法或者边界元法)很难描述该过程如有限单元法在大变形状况下会发生网

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。