连续法

In Part 1 of our research, BGSCs were sorted through immunomagnetic beads marking by CD133 and cultured in vitro, and character as a stem cell was identified by stem cell markers (CD133 and Nestin) and differentiated cell markers [ microtubule-associated protein 2(MAP2), glial acidic fibrillary protein and myelin basic protein] , ultrastructure observing with electron microscopeand engrafting to severe combined immunodeficiency mice for tumorigenesis test. The results were as following: Only a small subset of CD133+ glioma cells in glioma cell lines and fresh specimens from various pathologic grade could express stem cell markers CD133 and Nestin, view ultrastructure of a stem cell and be capacity of serial passage in culture. These CD133+ cells possese a marked capacity for multipotent differentiation and could differentiate into tumor cells expressing MAP2,β-TubulinⅢ, GFAP and MBP; When engrafted into SCID mice, they can generate and form tumors that phenotypically resembl the tumor from the patient.

在本课题中，在第一部分实验中采用以CD133为标志的免疫磁珠法从人脑胶质瘤组织和细胞株中分离脑胶质瘤干细胞并进行体外培养，通过免疫荧光技术检测干细胞标志物CD133、Nestin，诱导分化后检查分化细胞标志物MAP2、GFAP、MBP以及电镜超微结构观察和移植SCID鼠致瘤实验，对其干细胞特性加以鉴定，得到如下结果：不同病理分级的新鲜胶质瘤标本和胶质瘤细胞株中存在一小部分CD133+的胶质瘤细胞，能表达干细胞的标志物CD133和Nestin，符合干细胞的超微结构特点，体外培养能连续传代；具有多向分化潜能：诱导分化后能产生MAP2、β-TubulinⅢ、GFAP、MBP染色阳性的细胞；移植SCID鼠后能形成与亲本肿瘤表型一致的移植瘤。

After treatment for 60 days, the rats were sacrificed and the sperms were isolated from the caudal epididymides of rats with Klinefelter diffusion method. Effect of THH on the motility, appearance, number and density of rat sperms was evaluated by CASA system.

连续喂服60 d后，采用Klinefelter扩散法收集大鼠附睾尾精子，采用CASA系统观察精子计数、密度、形态、活动力。

By adopting strain intenerated model and using numerical calculation of FLAC(Fast Lagrangian Analysis of Continue), deformation and destruction course under pick cutting is researched, different parameters' great influence on plasticity district, max displacement, max main stress and max shearing stress under the various kinds of operating modes is analyzed.

采用应变软化的模型，使用连续介质快速拉格朗日分析法(FLAC，FastLagrangianAnaly-sisofContinue)进行数值模拟计算；研究截齿截割作用下煤体变形破坏过程；分析各种工况下不同参数对煤体塑性区、最大位移、最大主应力、最大剪应力的影响。

Adult Wistar rats, supplied by the Animal Center of that university,were divided by random lotting into control group, acute hypoxia group, and chronic hypoxia group.Hypobaric chamber was used to simulate hypoxia for 2and 30 days at an altitude of

成年Wistar大鼠，校动物中心提供，按随机抽签法分为对照组、急性缺氧组和慢性缺氧组，低压舱模拟海拔5 000 m连续缺氧2，30 d，复制动物急性和慢性缺氧模型。

In order to discuss the effect of overburdens under injecting grouts in separate layers on the morphology of the water flowing fractured zone, continuous exploration for failure characteristics of the overburden of working face 14308 of Dongtan Coal Mine under injecting grouts in separate layers and fully mechanized sub-level caving condition was processed by the method of underground uphole water injection technology by intervals with bore-head leaking multi-loops affusion system.

为了探讨覆岩离层注浆条件对导水裂隙带形态的影响，采用前端泄露式多回路注水系统，应用井下仰孔分段注水法对东滩煤矿14308工作面覆岩离层带实施注浆充填条件下综采放顶煤导水裂隙带的破坏特征进行了连续探测。

The quality concentrations of serum E2 were detected by radioimmunoassay. Pituitaries were embedded by paraffin and sectioned continuously. The morphologic figures of pituitary were shown by hematoxylin-eosin stain, the expression of estrogen receptor and progesterone receptor were detected by immunohistochemistry.

术后40 d麻醉后处死动物，放射免疫法检测血清雌二醇质量浓度，垂体组织常规石蜡包埋，连续切片，分别进行苏木精-伊红染色和雌、孕激素受体免疫组织化学染色。

In addition, breakdown parameters near the solid wall are reinvestigated by a detailed kinetic study and a new criterion of breakdown parameter is proposed.

藉由使用直接蒙地卡罗法进行详尽的分子动力学研究重新检视，在靠近固体边界区连续流假设失效参数的使用，并且提出新的连续体假设失效与否的新标准。

In this article, the process of snitched continuous glass fiber mat reinforced PVC composites by aqueous suspension is studied.

介绍了水悬浮法制备连续玻璃纤维针刺毡增强PVC复合材料的成型工艺。

It also eliminates the invalid paths between statuses considering the restriction of minimum continuous time of on and off , and tamps rates , thereby reducing the number of paths in dynamic programming and attaining the aim of reducing dimensions . After confirming the running or suspended status of units , the approach is used to distribute powers among units , thereby increasing the efficiency of dynamic programming greatly .

通过比较各时段负荷及机组参数，剔除各时段下不满足要求的组合状态，从而减少动态规划中的状态点数；根据机组的最小连续运行，停运时间限制，计及机组功率上升，下降速度的约束，剔除了状态点间的无效路径，从而减少了动态规划的路径个数，达到降维的目的；在确定机组启停状态后，再采用解析法进行机组的功率分配，可以大大提高动态规划方法的效率。

Firstly,based on the WR Sexton method,the method and mathematic models of supplementing the sub-idle rotating component characteristics is presented.Secondly,the turbofan windmilling high-low pressure rotor co-working equations and the whole engine co-working equations are established.lastly,a turbofan engine windmilling characteristics under various windmilling conditions are simulated and analyzed.

首先，基于不可压流理论，附以可压缩效应修正的指数法发展了补充慢车转速以下部件特性的计算模型和计算程序；其次，根据流量连续、压力平衡、功率平衡、燃烧室进出口总温相等及转速相等等约束条件建立了风车状态时涡扇发动机高低压转子及整机的共同工作方程；最后发展了具有一定计算精度的涡扇发动机风车状态特性计算模型和程序，并以某型涡扇发动机为例，计算分析了不同飞行条件下发动机的风车特性，从计算结果看，其趋势是合理的。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。