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In this study, the structure sequences of Shiga toxin 2B(stx2B) and Shiga toxin 1B(stx1B) were respectively amplified from EHEC O157:H7 by PCR. stx2B-L-stx1B fusion gene was constructed by a 12 bp linker ligating these two fragments. After digested with restriction endonuclease Nco Ⅰ and EcoR Ⅰ, the fusion gene was orientally inserted into expression vector pET28a.

利用PCR方法,从肠出血性大肠杆菌O157:H7的基因组DNA中,分别扩增出Stx1B和Stx2B亚基的结构基因片段,然后再通过PCR将这2个片段连接起来,并定向克隆到表达质粒pET28a的NcoⅠ和EcoRⅠ位点之间,构建了重组表达质粒pMB1。

He's piecing together two clips of the same action so it's one quick cut.

他在两段动作相同的短片连接起来

The shell polyose is glucosamine is composed by the majority of D- glucosamine and few N- the acetyl - D-, gets up the linear chain polysaccharide by β-(1,4) sugar glucoside keying, its structure is similar to the cellulose.

壳聚糖是由大部分D-氨基葡萄糖和少量的N-乙酰-D-氨基葡萄糖组成,以β-(1,4)糖苷键连接起来的直链多糖,其结构类似于纤维素。

In October 1995, a new bridge-the Fengpu Bridge was erected over the Huangpu River connecting Punan and Fengxian, and therefore becoming the fourth fully constructed bridge that opened for traffic after Xupu, Nanpu, and Yangpu Bridge.

1995年10月,黄浦江上又一座大桥凌空飞架,将浦南与奉贤连接起来,成为继徐浦,南浦,杨浦三座大桥之后建成通车的第四座大桥——奉浦大桥。

Raman spectrum and UV-vis-NIR absorption spectrum show that the above procedure does not change the electronic structures of single-walled carbon nanotubes. Moreover, the infrared spectrum confirms the formation of amide bond between ammonium sulfamate and carbon nanotubes.

Raman光谱和UV-vis-NIR吸收光谱表明,上述处理过程没有改变单壁碳纳米管的电子结构;此外,红外光谱的研究说明氨基磺酸铵与碳纳米管是通过酰胺键连接起来的。

That road is builting will have finished this year . it will jion up two townlet together .

正在修的那条路今年会完工,它将把两个小镇连接起来

The goal of Grid resource management is integrating all kinds of autonomic resources and applications,and managing them unitedly to provide expandable,security,unanimous,pervasive,high efficency and wide scope resource sharing and computing service.

网格的发展和应用对网格资源的发现和管理提出了新的要求,网格资源管理的目标是要把通过网络连接起来的各种自治资源和系统进行集成并统一管理,提供可扩展的、安全的、一致的、普及的、高效的大规模资源共享和计算服务。

You see Lake Ontario on one side and Lake Erie on the other, and linking them the 34-mile Niagara River.

你看到瀑布的一边是安大略湖,另一边是伊利湖,把这两个源连接起来的是34英里长的尼亚加拉河。

You see Lake Ontario on one side and Lake Erie on the other, and linking them the 34-mile Niagara River.

你看到瀑布的一边是安大略湖,另一边是伊利湖,把这两个源{本文出自:www.zhonguokw.cn中国考网。cn}连接起来的是34英里长的尼亚加拉河。

Visual C++6.0 and SQL that the system adoption acquaints with are the development tools of system, and pass to connect and transact a software conjunction to get up in common usely in the system, making the function of system is more strong.

系统采用熟悉的Visual C++6.0和SQL作为系统的开发工具,并且在系统中通过接口跟常用的办公软件连接起来,使得系统的功能更加强大。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力