连接物

To do this, remove hose coupling and lightly pull knurled tip of stem at outlet of valve to allow drainage of collected water.

要增加防冻型号HB-1，先断开软管连接，轻轻拉出位于阀门出口处的阀杆旋钮状小突出物，让积存的水排出。

At aim of getting the full 235 bp gene, five 50~70 bp model-based primers were first synthesized by chemical method followed by 3-step PCR amplification in an effort to ligate them into full gene.

为了得到目的肽全基因，首先合成5条50～70 bp互为模板引物的DNA片段，再通过PCR扩增将5条片段连接成全基因。

CMTC crystal belongs to metallorganic complex compounds and growth units are in the form of complex anions. Therefore, there are probably some unsaturated electrovalent bonds on the growth face, which undergoes a "restruction" process by bond linkages until a stable state with least surface energy is reached.

2CMTC晶体是配合物型晶体，以配位阴离子基团形式向生长界面堆积生长，因此在界面上很可能会存在着一些不饱和的离子悬键，悬键之间可能相互连接进行&重构&以达到表面自由能最小的稳定状态。

During our strategy, two linear amplification approaches are employed, ligase detection reaction and an isothermal amplification (cooperation by DNA polymerase and nicking enzyme).

连接产物由于在DNA聚合酶和限制性内切酶的共同作用下，发生等温扩增反应，扩增出大量的血红素的适配子，适配子与血红素结合，形成的复合物催化鲁米诺和双氧水的化学反应。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Thedegenerate primers were designed and synthesized according to the sequence of NDHB amino acid published in GneBank. A novel cDNA encoding the second subunit of NADH dehydrogenase was amplified from Fraxinus velutina Torr by RT-PCR.

根据GenBank上已发表的NDHB氨基酸序列，设计合成了一对简并引物，采用RT-PCR技术对绒毛白蜡NADH还原酶第二亚基基因进行扩增，将扩增的PCR产物与pMD18-T连接后转化至E。

Secondly, the genomic DNA is extracted from Zostera marina L. and then amplified by PCR with two oligonucleotide primers synthesized to obtain the partial nucleotide sequences of the Na~+/H~+ antiporter gene. The Na~+/H~+ antiporter gene clone was obtained after a series of manipulation: purificatioin of PCR product, ligation and transformation of recombinant plasmids, blue-white selection and so on. DNA sequence analysis shows that its DNA sequence is highly homologous with the genomic DNA sequence of E.

其次，通过提取大叶藻基因组DNA，根据设计的简并性引物进行PCR扩增，纯化PCR产物，连接，重组质粒转化，蓝白斑筛选等操作，并测序，提交测定序列到NCBI进行BLASTN，结果表明：该序列与大肠杆菌的基因组序列相似性较高，而与其它物种的Na~+/H~+逆向转运蛋白基因没有相似性。

Electron microscopic findings were: 1. alveolar type I cells were degenerated、 broken-down and desquamated, endothelial cells were swelled, with inter cellular tight junction shortened, alveolar type II cells hyperplastic, basement membrane thinned and deformed; 2. alveolar macrophages and interstitial macrophages were hyperplastic; 3.mast cells were infiltrated and degranulated; 4.electron-dense deposits were present at alveolar wall; 5. myofibroblasts 、fibroblasts、 collagen and basement membrane like material were hyperplastic.

电镜观察可见：(1)I型肺泡上皮细胞变性、崩解和脱落，内皮细胞肿胀，细胞间紧密连接短小，II型肺泡上皮细胞增生，基底膜变薄和破坏；(2)肺泡巨噬细胞、间质巨噬细胞增多；(3)肥大细胞浸润并见脱颗粒现象；(4)肺泡壁电子致密物沉积；(5)肌纤维母细胞、纤维母细胞、胶原原纤维及基底膜样物质增生。

As the serpents leave, the ends of the etheric body that had been severed can be reconnected.

当蛇形物离开，被切断的以太体两端就能重新连接。

Chiral SalanCrX complexesa saturated version of SalenCr(ⅢX are designed,and in conjunction with an ionic quaternary ammonium salt or organic strong base can efficiently catalyze the asymmetric,regio-and stereoselective alternating copolymerization of CO_2 with rac-PO at mild conditions to afford isotactic-enriched polycarbonates with～95%head-to-tail linkages and moderate enantioselectivity.

本论文在Salen构型配体的基础上，设计合成了一系列手性Salan型铬配合物，并将其与季铵盐/有机强碱组成亲电-亲核双组分催化体系，在室温下可以有效的催化CO_2和环氧丙烷的不对称交替共聚反应，实现了CO_2和环氧丙烷的不对称交替共聚反应的区域和立体化学控制，得到95%的头尾连接单元和中等光学纯度(对映体过量值ee%≈70%)的全同结构的聚碳酸酯。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。