连接物

The calculated results showed that the decrease of the pyridylene content and the para-linked pyridylene in the backbone of the polythiophene resulted in a narrowed energy gap and bathochromic absorption and emission peaks.

另外，共聚物主链中吡啶成分减少以及吡啶的对位连接导致了能隙的变窄和吸收及荧光波长的红移。

In the intercellular space of the hepatocytes and tight junctions of bile canaliculi, TPPase reaction could be seen.

肝细胞间隙和毛细胆管紧密连接区可见TPPase反应物。

And SDCh, poloxamer, sodium caprylate, PEG-1500, Brij-78 and chitosan could decrease the membrane electro resistance and this effect is favor of rHV2 transport by paracellular way.

这一点提示可能存在穿过紧密连接被动扩散的吸收机制。B-A方向上转运量没有增加，提示rHV2不是P-糖蛋白的底物。

Many literatures focus on preterm prediction study and evaluate every predictor. The predictors include cervical length and the size of some of fetal organs measured by ultrasound; the level of fFN, IGFBP-1, IL investigated in cervicovaginal fluid, serum relaxin, CRP and HbA1c in mother's blood, MMP-8, MCP-1 and ITAC in amniotic fluid which correlated with infection.

早产的预测是研究热点，大量文献分别从超声测量学角度，包括宫颈长度，胎儿某内脏的测量；宫颈阴道分泌物中多项指标，如胎儿纤维结合蛋白、磷酸化及脱磷酸化胰岛素样生长因子连接蛋白-1、白细胞介素的测定；血生化分析，如血清松弛肽、C反应蛋白、糖化血红蛋白含量的变化及羊水化验与感染有关的一些因子，如快速基质金属蛋白酶8、单核细胞趋化蛋白-1、γ干扰素诱导T细胞α趋化因子等，阐述各指标对早产的预测价值。

The results presented here demonstrate that the highly specificantibody-antigen interaction can be used to generate single-moleculemaps of specific types of molecules in a compositionally complexsample while simultaneously carrying out high-resolution topographicimaging.

为了能够在新模型中应用原子力显微镜，他们在原子力显微镜的尖端粘上针对单个蛋白的抗体。为了保证抗体探针是真正敏感的，用一个多聚物把抗体和尖端连接起来，使抗体摆动到与蛋白受体更易结合的位置。

Here, we have pioneered the SINEs study in cyprinidae family based on the above questions, the results following:1 In this study, we firstly apply the inverse PCR protocol for isolating the SINEs from Opsariichthys bidens genome on the basis of A-B PCR.

马口鱼的基因组DNA利用HaeIII酶切后，利用T4连接酶进行自环化；环化后的DNA分子在反向引物的引导下执行反向PCR，最后，一组衍生于tRNA的SINEs从马口鱼基因组内获得，并根据马口鱼的学名Opsariichthy bidens命名为Opsar。

In order to develop antibacterial peptide biologics for prevention and control of endometritis of dairy cow,a pair of primers was designed and synthesized according to the sequence of bovine antibacterial peptide gene published in GenBank.The total RNA was purified from the dairy cow endometrial cells with the Total RNA Isolation Kit.

为研发防治乳牛子宫内膜炎的抗菌肽生物制剂，根据已发表的牛抗菌肽基因序列设计了1对引物，用试剂盒提取乳牛新鲜子宫内膜总RNA，经RT-PCR扩增，将克隆的抗菌肽BNBD5基因全长编码区的cDNA连接到原核表达载体pET30a中，经IPTG诱导原核表达，通过Western-blot检测融合蛋白BNBD5的表达水平。

In Chapter 3, six mixed-valence or heteronuclear metal imidazolates have been synthesized and structurally characterized through introducing univalent metal ion into bivalent metal imidazolates, which give a rich topological chemistry including novel topological networks.

其中尤为突出的是混价铜咪唑配合物的控制合成，通过精确调节反应条件得到了混价比例不同的产物，其中一种呈现出新型的四连接网络的拓扑类型。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

Gap junctional intercellular communication is important in the regulation of cellular proliferation and tissue homeostasis.

内容摘要近年来的研究认为抑制细胞缝隙连接间通讯是促癌物的一种重要特性。代谢协同试验是测定GJIC的方法之一。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。