连接时间

Its efficiency is testified by an application. The fixtureless tester can perform 100% test for PCB's connectivity through the probe movement. It takes a long time to test a PCB with low efficiency.

传统的飞针式在线测试仪必须通过大量的机械运动才能实现印刷电路板连接故障的完全测试，因此完成一块印刷电路板的测试需要较长的时间，检测效率较低。

The experience of gasless laparoscopic surgery using Home-made Abdominal-wall-take-up;2. A home-made capillary interface has been coupled with a high resolution orthogonal-injection time-of-flight mass spectrometer.

介绍了一种自制的毛细管真空接口，用于连接电喷雾离子源(electrospray ionization，ESI)与自制高分辨率垂直引入式飞行时间质谱仪，初步研究了该接口对仪器灵敏度的影响。

Results ① Normal morphological structure of brain microvascular endothelial cells and neuroglial cells were injured by 1,2-dichloroethane, which damaged blood-brain barrier.

结果］染毒后的脑微血管内皮细胞呈进行性破坏，随着染毒剂量的加大细胞体积逐渐增大，胞膜模糊，细胞与细胞间、细胞与突触间连接减少，甚至消失；随着染毒时间的延长，在10、30、60min后可以观察到有进行性的细胞膜溶解破坏，胞核模糊，界限不清，突触变短。

Herein, a series of compounds, BP-C〓-NBD (n=2, 3, 4, 5, 6, 8, 10), was synthesized. Nanosecond flash photolysis, phosphorescence emission and lifetime, as well as photoisomerization reaction of norbornadiene were used to understand the intramolecular triplet energy transfer in detail.

我们设计、合成了以多亚甲基连接的二苯酮与降冰片二烯（BP-C〓-NBD，n=2，3，4，5，6，8，10）系列化合物，利用纳秒时间分辨技术、低温磷光发射、磷光寿命以及光敏异构化的方法，对其分子内三重态能量传递过程进行了详细地研究。

Under scanning electron microscopy, along with the extension of the culture time, the chondrocytes grew and propagated well on the surface. On 3 weeks, the cells adhered to the surface, and extended the pseudopodia, by which the cells were connected each other. The cells secreted matrix, which deposited around the cells and the surface of meniscus. In some area, the cells formed the structure like cartilage lacuna.

扫描电镜观察，随着培养时间延长，细胞在材料表面生长良好，细胞密度增加。3周时，可见细胞黏附在材料表面，有伪足伸出，细胞间通过伪足相互连接，并可见细胞分泌的大量基质沉积于细胞周围和材料表面，部分区域形成类似于软骨陷窝样结构。

You can then reconnect later and see what has happened on the channels while you were away.

过会儿你可以重新连接，并检查在你离开这段时间通道中发生了什么。

In: Proceeding of Computer Vison and Pattern Recognition, New York, USA: IEEE, 2006. 2110–2117 2 Kim N W, Kim T Y, Choi Jong-Soo. Edge-Based Spatial De- scriptor for Content-Based Image Retrieval.

而基于Boosting学习的识别算法以区分性模型作为基础，旨在区分相册内不同人物，能够降低时间因素的影响以期把所有的时间段连接为一个整体。

Animals, as material, were used to study the toxicity of outer substance and made sure the estrogen effect of BBP by whole animal experiment, and the chronic toxicity of BBP was studied, designed and valued through several dosages and different exposed time. The result showed that BBP could make testis, epididymis and prostate withdrawl. Tectology changes were observed in testis, epididymis and liver. Especially the atrophy and degeneration of seminiferous tubules, decrease of spermatogenic cell and the turbulence of Sertoli cell and spermatogenic cell of seminiferous epithelium were also observed. The linkage between cells vanished.

明确了BBP的雌激素效应，并分别用多剂量和不同染毒时间来研究、设计、评价BBP在体内的慢性毒性，结果表明：BBP能引起雄鼠睾丸、前列腺、附睾性腺的萎缩和肝脏肿大，改变睾丸、前列腺、肝脏的组织形态学，尤其引起睾丸曲细精管萎缩、变性，各级生精细胞减少，生精上皮内生精细胞和Sertoli细胞结构明显紊乱，细胞间连接结构消失等。

On day 3, 7, 15, 30 and 90, renal biopsies were performed. Renal histology was checked under light microscopy with HE and Masson's trichrome staining sections.

在肾炎不同时间点(3、7、15、30和90 d)行肾活检，HE和Masson's染色，光镜观察肾组织形态，免疫组化ABC法检测Mф、纤维连接蛋白、Ⅲ和Ⅳ型胶原。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。