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One view is that the nucleocapsids get tegument in the nucleus, and by budding into perinuclear space, they obtain their envelope from the inner lamella of the nuclear membra...

一种意见认为,核衣壳在细胞核内获得被膜,并通过出芽在内层核膜处获得其囊膜后,进入核周间隙,进一步经与核周间隙相连接的内质网,以囊泡转运的方式经高尔基体到达细胞膜,囊泡膜与细胞膜融合,病毒颗粒释放到细胞外。

Objective To investigate the age distribution of the active trachoma and evaluate the significance of trachoma classi-ficltion and ligase chain reaction.

目的 了解活动性沙眼患者的年龄分布,评价WHO简易沙眼分级法和连接酶链反应诊断沙眼衣原体的意义。

METHODS: MyoD cDNA fragments were extracted from plasmids pEMSV-MyoD with polymerase chain reaction, and PCR was used to clone the whole-length gene of MyoD. After adding CACC sequence at 5' end, MyoD gene was cloned by orient topology into transfer ventor, pENTR/D-TOPO. Objective gene was transferred into adenoviral expression vector DNA via pENTR/D-TOPO vector. The recombinant adenoviral vectors transfected into HEK293A cells by using lipofectamine were packaged and amplified.

从pEMSV-MyoD质粒上用聚合酶链反应法扩增出MyoD cDNA片段,再通过聚合酶链反应使MyoD基因加上CACC序列接头,经过定向拓扑克隆使目的基因连接到转移载体上,再通过LR酶促反应,将目的基因转移到腺病毒表达载体DNA上,获得MyoD基因重组的腺病毒DNA,用脂质体转染法转染HEK293A细胞,包装扩增出MyoD基因重组的腺病毒。

The coordination polyhedron around Ce3+ is a distorted square antiprism, and the two independent molecules are connected by π-π stacking interactions to form a noncentrosymmetric dimer.

中心Ce3+的配位多面体为变形双帽四方反棱柱,两个分子之间以π-π堆积连接,生成一个非中心对称的二聚体。

METHODS: The total RNA was obtained by TRIzol from an antiricin neutralizing monoclonal antibody hybridoma cell named 4C13 and heavychain and lightchain variable fragments were amplified by overlap PCR and accepted by Genbank of NCBI. VH and VL were linked by3 with a direction of VHlinkerVL and cloned to pCDNA3.1 vector expression system.

用TRIzol提取抗ricin中和性单抗杂交瘤细胞4C13总RNA,扩增其轻、重链可变区基因,登陆GenBank;用Linker3将VH和VL连接,用重叠延伸PCR克隆入真核表达载体pCDNA 3.1,用脂质体转染293T细胞,对瞬时表达产物进行生物学活性检测。

You can enjoy music in anywhen and anywhere .

便携式皮式音响:内置超扬声器的音箱包方便又时尚,包体材料手感好,可以保护你的MP3被刮伤和丢失;方便的音箱包,可以和MP3 /随声听/ CD /个人音响/笔记型电脑或其它的音响设备连接;音质响亮且清晰,不需要耳机,你可以在任何时间,任何地点尽情的享受音乐带来的乐趣!

But Armillaria has the unique ability to extend rhizomorphs, flat shoestringlike structures, that bridge gaps between food sources and expand the fungus's sweeping perimeter ever more.

但奥氏蜜环菌有独特的生长方式:延伸菌根,连接食物源与菌体,然后迅速向外生长。

Since the Astral plane touches upon every other plane, you can travel astrally to other planes as you will.

因为星界又与其他位面连接,所以你的星界体可以在这些位面自由旅行。

From the ester n, the intermediate Ⅲwas produced by the Sonogashira coupling reaction with terminal alkynes under the co-catalysts of Pd2 and Cul. These final products of alkynyl benzamide derivatives IV were obtained by the cleavage of support with primary or second amines.

液相合成兼容了固相合成可快速简便地对产物进行分离纯化以及溶液相合成可在均相条件下进行反应和用常规手段对反应中间体进行分析检测的优点,用聚乙二醇4000作为可溶性聚合物支持体,通过醚键将[1,3,5]氯三嗪连接在PEG上制备成PEG支持的新型的可溶性聚合物试剂I。

We introduce two pyridinedicarboxylate (2,5-H2pdc and 2,6-H2pdc) ligands, under hydrothermal or solvothermal condition, to yield 7 complexes with InCl3. Complex 17 presents a rare example of chiral binodal (4,8)-connected lattice with self-interpenetration.

选择了两种吡啶二羧酸配体: 2,5-吡啶二羧酸(2,5-H2pdc); 2,6-吡啶二羧酸(2,6-H2pdc)和InCl3反应合成了七个配位化合物其中17表现了一例少有的手性的(4,8)连接的双节点自穿插拓扑结构。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力