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Insect resistance to Bt toxin is related with the variation of cadherin-like protein gene.cDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique,and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1 335 bp的片断编码444个氨基酸残基。

I long to alleviate the evil,bue i can not,and i too suffer.

我渴望能够减少邪恶，但是我无能为力，甚至连我自己也再劫难逃。

What the hell,' he said, 'with the President's death you could execute buggery in the streets and get no farther than the fourth page.'

真他妈见鬼，'巴顿说，'总统去世意味着你可以大街上搞男人，而且连第4版都上不了。'

In China, not only Cadillac but even Buick, a dismal mid-market brand at home, have allure.

但在中国，不仅是凯迪拉克，就连在美国本土只是一个表现平平的中端品牌的别克，也变得有吸引力了。

She needs a building permit for her girdle.

你妈妈胖到连她的腰带都要建筑执照。

Bulger EM,McMahon K,Jurkovich GJ.The morbidity of penetrating colon injury［J］.

张连阳。重视严重多发伤救治中的损害控制外科细节［J］。

In 1992, I helped start up and edit Wired magazine - the official bullhorn of digital culture.

随后，我加入了创建当时新兴互联网第一个大众门户网站的工作之中。1992年，我帮助创建并编辑了《连线》杂志——数字文化的官方扩音器。

Today, in contrast, we are not seeing anything like the stubbornly-held bullishness that was so widespread then.

然而今天的情况却是相反，我们并没有看到什么所谓顽固看涨的情绪，甚至连看涨也根本说不上。

I don't ever get enough to eat, gen'ally -- and here they can't come and pick at a feller and bullyrag him so.

平常我连顿饱饭也没吃过——而且这儿也没谁来欺负你。

For the enlightenment of those who are not so intimately acquainted with the minutiae of the municipal abattoir as this morbidminded esthete and embryo philosopher who for all his overweening bumptiousness in things scientific can scarcely distinguish an acid from an alkali prides himself on being , it should perhaps be stated that staggering bob in the vile parlance of our lower class licensed victuallers signifies the cookable and eatable flesh of a calf newly dropped from its mother

这位有着病态精神的审美学兼胚胎哲学家，尽管连酸与碱都分不清，在科学知识上却摆出一副傲慢自负的架子。为了启发那些对市立屠宰场的细节没他那么熟悉的人们，也许应该在此说明一下：我们那些拥有卖酒执照的低级饮食店的俚语小牛崽肉，指的就是打着趔趄的牛崽子280那可供烹调食用的肉。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。