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MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

The quality of the continuous casting slabs is very important to the production of the continuous casting machine and the developm...

连铸坯的质量对连铸生产和连铸技术的发展是至关重要的问题。

The quality of the continuous casting slabs is very important to the production of the continuous casting machine and the development of continuous casting technology.

连铸坯的质量对连铸生产和连铸技术的发展是至关重要的问题。

Unseating prevention device is an important component of collapse-proof system of highway bridges.

连梁装置是公路桥梁防落梁系统的重要组成部分之一，本论文在分析桥梁震害及其原因、介绍国内外连梁装置的发展和现状的基础上，设计了钢棒和钢绞线连梁装置。

Based on copper plastic processing features, the metal rolling theory and the practical procedure, the paper presents the design method of the copper bar rolling mill pass system and the calculation methods of the factors. Some key factors, such as the elongation factor and filling metal degree through the pass system at every rolling segment, and some dynamic factors are calculated for small and middle copper bar rolling mills.

根据铜加工塑性加工特性及金属连轧理论，结合生产过程，文章提出了中小直径铜杆连轧机整个孔型系统设计方法和参数计算方法，计算了各轧制道次延伸系数、各道次孔型金属充满度等关键参数，并对连轧机中的动态参数进行了计算。

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Methods the angles from the body surface of yamen (gv 15) to the joint line between the level opposide atlas and axis, and the joint line between body surface of yamen point and posterior margin of the great occipital foramen, upper margin of arcus posterior atlantis were measured respectively in 1120 cases of t1 weighted image of median sigittal position with mri, and the data were processed by computer.

取1120例mri正中矢状位t1加权像，分别测量体表哑门穴至水平相对寰、枢椎椎间的连线与体表哑门穴至枕骨大孔后缘连线、寰椎后弓上缘连线所夹的角度，并由计算机进行数据处理。

Accurate and powerful, Today's Assault Rifles usually have calibres ranging from 5.45mm to 7.62mm, a magazine capacity of 20-30 or more rounds, selective full auto and single shot modes of fire, and in some models, 2 or 3 round burst mode .

突击步枪是现代军队的中流砥柱，当今的突击步枪精准且杀伤性强，有5.45到7.62毫米各种口径，单弹匣能容纳20到30甚至更多子弹，射击模式有连发、单发，有的枪械还能2连发3连发。

The paper transferring mechanism includes a drive gear coaxially coupled with a rotation shaft of a motor, a pick-up gear coupled with a shaft of a pick-up roller to separately feed the paper sheets, and a feed gear coupled with a shaft of a feed roller to outwardly transfer the paper sheets.

一种纸张传送机构，包括：与一电动机的旋转轴同轴耦连的驱动齿轮，与一拾取辊的轴耦连以分别供给纸张的拾取齿轮，与一送纸辊的轴耦连以向外传送纸张的送纸齿轮。

Vandervoot sent F Company across the river, to support E Company with a flank attack, but it had little effect on Peiper's men and tanks, who were massing for a decisive attack.

万德沃中校派出F连过河，以侧翼进攻支援E连，但是F连的攻击行动对佩普的士兵和坦克没啥影响，后者依然抱成团发起拼死进攻。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。