进行试验

Study of experimentation, in which the medicament acted on the resistance and occlude action of venomousness and poisonous ingredient of gangue-brick, showed that the medicament on gangue-brick had the effect of pervading and lixiviating the venomousness ingredients.

在制砖扩大试验中，对实验室试验制砖的各项技术指标的最佳条件进行了验证，考查其重现性及误差；对扩试设备运行的连续性、稳定性和可靠性进行了考查；对试验中砖制品出现的技术问题进一步补充、完善，使扩试砖制品质量稳定并达到国家相应建材标准；根据扩试结果进行经济成本估算等，使尾矿制砖技术更趋完善。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Connected with cross section patterns of freeway, the design method of drainable base course system and edge drainage system which meet to request are given too. The aggregate grading and mixture ratio of porous cement-treated macadam are obtained based on accurate survey on material properties and lots of laboratory experiments. After the construction of experiment road, a summary of mixing, spreading, roller compaction and maintenance process about subsurface drainage system is made, and the analysis on the cost of construction about drainable base course is also carried. According to the test of coefficient of permeability, porosity and strength of compression about local test samples and core samples, the paper concludes that main performance index of subsurface drainage system has achieved the design anticipated effect.

结合高速公路横断面型式，提出了适合要求的排水基层排水系统和边缘排水系统设计方案；在进行详细材料调查和大量室内试验的基础上，提出了多孔隙水泥稳定碎石基层的集料级配和混合料配合比；修建了路面内部排水系统试验路，根据试验路工程的施工情况，对路面内部排水系统施工的拌和、摊铺、碾压和养护工艺进行了总结；并对排水基层造价进行了分析；通过对现场取样试件、芯样的渗透系数、孔隙率和抗压强度的测试，证明路面内部排水系统的主要性能指标均达到了设计预期的效果。

SD rat, rabbit and guinea pig were used to test acute toxic, stimulative, and hypersusceptible reaction in treated by compound Daihuang ointment, respectively.

利用健康SD大鼠进行皮肤急性毒性试验；用健康大耳白兔进行皮肤刺激性试验；用健康豚鼠进行皮肤过敏试验。

The antifatigue function of more than forty kinds of insect,fungi,bacteria and algae was studied and sifted first in this study by detecting swimming endurance and biochemical indexes. Then,in order to choose the better ones from the compoud additives formulas composed of L-carnitine, taurine, octacosanol,vitaminE and insectA ,a cross design experiment was done. InsectA was discovered in the first experiment that it greatly improved the antifatigue function in mice. Finally, the acute toxicities, thirty-day feeding effects and the mechanisms of No. 1 antifatigue compoud additives(No.1ACF) were studied.

本研究首先通过小鼠游泳耐力试验和血液生化指标的测定对40余种昆虫、菌藻的抗疲劳作用进行了筛选；在此基础上，应用正交设计方法对以L-肉碱、二十八醇、牛磺酸和本实验筛选出的抗疲劳功效较好的昆虫A为原料的配方进行了抗疲劳作用筛选，并对筛选出的最佳配方，即抗疲劳复方添加剂1号配方进行了小鼠急性经口毒性试验、30天喂养试验和抗疲劳作用机理研究。

A lot of experimental data have been accumulated to verify the model, including plastic damage under uniaxial tension, cyclic deformation behaviors and fatigue data for copper experimented by the author and biaxial deformation experiments done by Lamba [87] etc..

本文通过大量的试验资料对提出的模型进行了试验验证，包括黄铜单位塑性损伤、循环加载变形特性及疲劳寿命试验，另外还收集了国内外众多的试验数据对模型进行验证。

At first a the fundamental experimental research has been performed, such as resistance performance of system, the effect of Ca/S mole ratio, approach to saturation temperature , SO〓 inlet density, the flow rate and humidity of flue gas on the desulfurization efficiency, and then the studies on high-rate circulation of solid particles in the desulfurized tower were done, overwet phenomenon were analyzed; the study on carbide slag desulfurization and scaling in the tower has been performed in detail.

本文在大型循环流化床脱硫的工业化装置上，进行了脱硫机理和试验研究，取得了大量的试验数据和运行经验。首先进行了进料钙硫比、近绝热饱和温度、SO〓入口浓度、烟气流速及烟气湿度等因素对装置脱硫效率影响以及系统阻力性能等等的基础性实验研究；进一步对脱硫塔内固体颗粒物高倍率循环对脱硫装置性能的影响、过湿现象、电石渣脱硫、团聚现象以及脱硫塔内结垢等问题进行了试验和机理性研究。

Three kinds of typical shells, such as scallop, whelk, bloody clam, as well as one kind of limestone were chosen to perform desulfurization and calcination experiments on the fluidized bed platform.The microstructures of the tested samples were mensurated by means of one mercury porosimeter.

在流化床上对3种典型贝壳---扇贝、海螺、毛蚶以及一种石灰石进行煅烧脱硫试验研究，并利用压汞仪测定试验样品的微观孔隙结构，通过最可几孔径、孔隙率、比表面积等3个结构特征参数对微观结构进行定量分析；另外，还对试验样品的化学成分进行了分析。

The results obtained with an improved asphalt pavement analyzer show that the fatigue life of SAWI is 3.32 times that of the common asphalt concrete. The pull-out test results obtained with a modified material test system indicate that SAWI is of good interlayer bonding capability. Moreover, as compared with the control sample, SAWI is of strong preflection crack resistance.

改进的沥青路面分析仪试验结果表明，SAWI的疲劳寿命是普通沥青混凝土的3.32倍；利用改进材料试验系统的夹具进行的拉拔试验表明，SAWI具有很好的层间结合能力；在与常规的加铺层进行的对比试验中，SAWI结构表现出了优良的抗反射裂缝能力。

Based on the current condition of Henan aluminum soil recultivation, we located an empty and waste area after exploited at Xiaoguan Mine of Changcheng Aluminum Industrial Company and carried out a series of experiment and research on soil recultivation there in terms of engineering recultivation, biological recultivation and soil ripeness.

依据河南省铝土矿土地复垦的现状，选取长城铝业公司小关铝土矿开采后的采空区、废石场进行土地复垦的试验和研究。主要进行了工程性复垦试验、生物性复垦试验、土壤熟化试验研究。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。