进行实验

In this paper, acetylsalicylic acid, cyclohexanone Preparation of micro-content of the experimental design, in different reaction conditions were carried out under analysis to optimize the improvements and chemical experiments on the basis of conventional experimental and micro-experimental comparison, being The experimental results consistent with the premise, fully reflects the micro-chemistry experiments to save time, save the amount of reagents, as well as many advantages such as reducing environmental pollution.

本文阐述了乙酰水杨酸、环己酮微型化制备实验的设计内容，在不同反应条件下进行了对照分析，从而进行优化改进，并就基础化学实验中常规实验与微型实验进行比较，在得到一致的实验结果的前提下，充分体现了微型化学实验节省时间、节省试剂用量、以及减少环境污染等诸多优点。

Our study contained three sub experiments: First, we looked into the music perception neural mode under the u...更多n-attention condition, then pre-attention condition, and attention condition at last. It suggested that:1. Similar to the past studies, ERAN was also acquired when irregular chords was played to both music experts and ordinary persons, with the former swings larger than the latter ones, and the former delitescence earlier than the latter ones.2. N5 was also acquired in the same condition, and it reflected the conformity process of music perception. The swings of experts' N5 were lager than the ordinary ones, and delitescence earlier.

本研究分为三个实验进行：实验一即在非注意实验条件下进行音乐感知能力的研究；实验二即在前注意实验条件下进行音乐感知能力的研究；实验三即在注意实验条件下进行音乐感知能力的研究，研究结果表明：1类似于前人的研究，不规则和弦在音乐专家和普通人中都产生了右前负极性，音乐专家的ERAN比普通人的振幅更大，潜伏期更早。2伴随着ERAN，不规则和弦在音乐专家和普通人中还产生了晚期负极成分(简称N5)，它反应了音乐的整合。

Therefore it is necessary to research the mechanism of greensand used for high compacted molding methods thoroughly.This subject is one section of the National Natural Science Foundation subject ""Ivestigation on Predicting Method and Mechanism of Springback for High Compacted Sand Molds"", followed of the other completed one named ""Research on Microdeformation of Molding Sand"". A serious of experiments has been done on the reequipped three axial machine under certain technological conditions, and the mechanics, material science, rheologic science. It is systematically studied that the influences on the springback of sand mold were caused by the main influence factors: pressure, compacted rate and the content of soil. Through the analyzed experiments' data, the experienced stress-strain relationship concerning the main influence factors were obtained;under different technical parameter, the springback and spingback's potential energy are compared, the differences and indifferences between them are found out, and the springback's mechanism is discussed;according to the tests' data of YangGuoping coming from Tinghua University, experienced formula of molding sand's springback displacement is induced, the displacement and springback are compared, the margin of error's reasons are analyzed, and they are tested and verified mutually.

本课题为国家自然科学基金项目"高紧实度砂型预测方法及回弹机理的探讨"的一部分，是已结题的国家自然科学基金项目"铸造型砂微变形的研究"的后继，运用力学、材料学及流变学理论，利用改装的三轴仪，在一定的工艺条件下，作了大量系列实验，系统的研究了压实比压、紧实率和膨润土含量对砂型回弹的影响；对实验数据进行了分析和处理，得出了型砂关于压实比压、紧实率和膨润土含量的本构关系的经验公式；把各工艺参数下的型砂产生的回弹量与回弹势能进行比较，找出两者存在的异同点，并对回弹机理进行了探讨；通过清华大学杨国平的实验数据，推导出型砂回弹位移场的经验公式，并与实验测出的回弹量进行比较，分析误差造成的原因，并互相进行了验证。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Situation of the utilization of experiment resources:(1) The opening up of the laboratory are relatively weak aspect, so, in these schools, students have few chances to do extra-curricular experiments;(2)Almost all of schools teaching experiment instruments are used, less instrument is not used, the main reason for not used is that these experiment instruments are irrelevant with materials or they are obsolete and damaged;(3) Teachers always experimentalize according to the teaching materials step-by-step, there is no improvement;(4) Most teachers don\'t pay attention to the evaluation of experiment result, students merely write expriment report;(5) The teachers have realized some significance of inquiry experiments, but have not begun action consciously.

从实验教学过程和实验资源开发利用情况看：(1)实验室的开放是比较薄弱的环节，绝大多数学校只有上实验课时才开放实验室，因此这些学校的学生进行课外实验和小制作的机会很少；(2)绝大多数学校实验教学仪器的利用情况较好，闲置仪器较少，其闲置的原因主要是实验内容与教材无关或仪器陈旧损坏；(3)实验过程基本上是按教材提供的步骤进行，教师对实验过程和教学仪器的研究和改进较少；(4)很多教师不重视对实验结果的评价，学生很少写实验报告；(5)教师对探究性实验的意义已有认识，但还没形成自觉行动。

In the pre-experiment stage, we analyze the colors and shapes of 135 visual illusions by Kitaoka, a Japanese scholar. The result is referenced in the formal experiment. Formal experiment includes three sub-experiments: Experiment 1 is to discuss the effect of the displacement of disjunctive center on visual illusions' movement; Experiment 2 is to discuss the effect of image layout on visual illusions' movement; Experiment 3 is to discuss the effect of color configuration on visual illusions' movement. Post-experiment stage is a repeat of formal experiment to confirm the results.

前测实验是进行日本学者北冈明佳的135幅错视动态作品之造形与色彩的分析，并将分析结果作为正式实验样本之参考；正式实验分成三个实验进行探讨，实验一在探讨分割点中心的位移对於错视图形在视觉动态上的影响，实验二在探讨图形配置对於错视图形在视觉动态上的影响，实验三在探讨色彩配置对於错视图形在视觉动态上的影响；而后测实验则是将正式实验之实验结果进行再实验，以印证正式实验之结果。

The algorithm is evaluated by using a large collection of real traffic matrices collected in Abilene network.

使用Abilene提供的网络流量矩阵进行实验，使用Totem模拟验证了MinMat算法选择结果的有效性。

You can already experiment with the code: cvs module " embed " in project gallery - contrib.

你已可以使用代码进行实验：&嵌入&于gallery-contrib项目中的cvs模块。

In this paper the research includes two parts. Firstly, CaO-SiO_2-Al_2O_3-CaF_2 is chosen as the basic slag. Effects of adding additives to CaO-based slag on melting point, viscosity and desulphurization ability have been investigated.

本次研究主要分为两个部分，第一部分对脱硫合成渣进行实验研究，选择CaO-SiO_2-Al_2O_3-CaF_2为基础渣系，在CaO基脱硫合成渣中加入添加剂，研究了添加剂对合成渣的熔点、粘度以及脱硫性能的影响。

The 0.5-scale water model based on Froude number similitude criteria was set up to experiment.

然后基于相似原理，利用修正的Froude准数相等，选取比例系数为1：2缩小比例模型进行实验研究。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。