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After hybridization of two DNA probes with target DNA, EDTA-〓 and β-diketone come close to each other, and an EDTA-〓-β-diketonate ternary complex having strong and long-lived fluorescence was formed, thus the target DNA was detected sensitively with a detection limit of 6 pM (0.6 fmol per assay) by time-resolved mode.

系统研究了使用EDTA-〓为标记物的石墨炉原子吸收光谱免疫分析法,并将之用于人血清中AFP和水中BSM的测定。该法中使用了EDTA-〓标记SA及生物素标记抗体或抗原,在96孔板中的免疫反应结束后,利用稀硝酸将反应产物分解,然后用石墨炉原子吸收光谱法对免疫反应产物进行测定。

In this study,we have identified C2H2 ZFP genes in the silkworm,and acquired the basic information for these genes,such as gene distribution on chromosome,gene family information and expression information.This information will be useful for further functional studies on these genes.

综上所述,本研究对家蚕基因组中的C2H2型锌指蛋白基因进行了鉴定,并通过分析获得了这些基因的基本信息,即染色体分布、基因家族信息和基因表达信息等,这些信息为进一步对家蚕C2H2型锌指蛋白基因进行功能研究打下了基础。

Meanwhile, Fernando Torres, Pepe Reina, Xabi Alonso, Alvaro Arbeloa and Albert Riera are in the Spain squad for their World Cup qualifying double-header with Turkey. The two teams will meet at the Bernabeu on Saturday, before doing battle once more in Istanbul the following Wednesday.

与此同时,利物浦阵中的西班牙五壮士:托雷斯、雷纳、阿隆索、阿贝罗阿以及里埃拉都相继入选了国家队,而他们也将与土耳其国家队之间进行连续两场的世预赛:首先是本周六,斗牛士军团先在伯纳乌迎战星月军团,随后双方将移师伊斯坦布尔再次进行鏖战。

Based on the theory of heat transfer, hydrodynamics and the theory of metal solidification, by using numerical analysis technology, database technology and visual technology, this paper provided a simulation and check method for the process design to simulate the fluent field and temperature field of the filling solidification and cooling, and can predict the casting quality according to these fields, thus, by this way, improved the quality of casting draft design.

该课题就是为工艺设计提供模拟和校核手段,以传热理论、流体力学及金属凝固理论等为基础,利用数值分析技术、数据库技术和可视化技术等对铸造过程进行仿真,从而模拟出在铸件充型、凝固和冷却中的流场和温度场,并根据这些物理场对铸件质量进行预报,从而提高铸件工艺设计的质量。

The harm of worms' outbreak becomes greater and greater. Such make worm detection and defense technology face an increasingly tough challenge. Attack Signature Automatically Generation technology is one of the key technologies, this paper focuses on the ASAG technology for research, its main jobs include: 1)Analyzed the research status of ASAG technology from the overall, described its design goals and evaluation criteria, and the current approaches' limitations is given. 2) After deeply researching and analyzing a various of ASAG systems, a basic framework for the network ASAG system is presented, and the detailed analysis of the main function of the components in the framework is also presented. 3)An attack capture model based on distributed honey-pots system is presented. This mode integrates the anomaly detection which based on the mode of dubiety score in the honey-pots, can do a second filter for the samples in honey-pots, and improve the purity of the samples captured. 4) A simple noise-resilience ASAG algorithm is presented, which used the behavioral characteristics of the worm outbreak to generate efficient signatures of samples in the suspicious dataset, which contains large amounts of noisy data, under the complex environment.

攻击特征自动提取技术是网络蠕虫防御技术的关键技术,本文围绕攻击特征自动提取技术开展研究工作,其主要工作包括: 1)对攻击特征自动提取技术的研究现状进行了综述,介绍其设计目标与评价标准,并指出了现有方法存在的局限性; 2)在深入研究与分析各种攻击特征提取系统的基础上,提出基于网络的攻击特征自动提取系统的基本框架,并详细分析框架中各部件的主要功能; 3)提出了基于分布式蜜罐系统的攻击样本捕获模型,该模型在分布式蜜罐系统的基础上,结合基于可疑度模型的异常检测,对蜜罐中的样本进行二次过滤,较好地提高了捕获样本的纯净度; 4)提出一种简单抗噪的攻击特征自动提取算法,该算法利用蠕虫爆发时的行为特征,在复杂环境下含有大量噪声数据的可疑样本训练集中提取有效的特征,降低对样本纯净度的依赖。

Earth because the Moon has just been caught near the Earth, the Earth in six plate gravitational pull of the moon, a strong collision, the crustal rocks in the hot rolling-out with the ice water for drastic changes in the physical and chemical.

因为月球刚被地球捕获,距离地球近,地球上6大板块在月球拉引力作用下,进行强烈碰撞,让地壳中火热的岩铁浆流出来,与冰水进行剧烈的物理和化学变化。

Using the flexibility of proteins, egg white albumin was pore-formed and heat-denatured in the presence of substrate template. BSA protein and herring sperm DNA were trasted by precipitation method in organic solvent.

利用蛋白质在水溶液中的柔性,分别采用热变性法对蛋清蛋白质进行生物印迹和沉淀法对BSA进行生物印迹,并在BSA印迹的基础上又印迹了鲱鱼精脱氧核糖核酸。

Some new viewpoints are also discussed besides the development of researches already explored. The above mentioned aspects include exploring ne w elementary technique, possible scheme for simplifying the structure, achieveme nt of dynamic area decomposing method, establishment of distributed parallel pro cessing system and application of similarity method in numerical simulation of w elding process.

除对本研究组已开展的工作进行了一定的分析外,并对某些未来的研究发展方向进行了初步的探讨,这些可能进行的研究包括单元技术的开发、可行的结构简化方案、动态区域分解算法的实现、分布式并行处理系统的建立和相似原理在焊接过程中的应用等。

The first is a discussion of "anxiety" and "depressing memory" from the level of psychology, spirit and culture, which are regarded as both obstacle and impetus of self-identity. Next is self-identity's image cast upon intimate relationship, that is, Electra complex, Oedipus complex, homosexuality and heterosexuality among women. The last is an analysis of narcissism that is the source and result of the above intimate relationship, which from individual narcissism to a age's or cultural narcissism, that is from sub-consciousness to historic scene, directly results in the plight of women writers and their writings.

首先对作为自我认同的障碍和动力因素的"焦虑"和"创伤性记忆"进行了心理、精神和文化层面的论析;其次对自我认同在亲密关系建立上的具象投射进行了分析和论证,即女性自我所表现出来的恋父情结、恋母情结、同性恋情结和异性恋,并指出其在女性写作发展中的历史表现;最后对作为以上亲密关系的根源和结果的自恋情结进行了分析,从个人的自恋到时代和文化的白恋,即自恋的从潜意识场景到达历史场景,直接造成了女性写作者和女性写作本身的困境。

Under the existing condition of the lab, a lot of experiments, analyzing and improving are made. Now, the problems in photoengraving, sputtering and diffusion etc. have been solved elementally. Excellent technological parameters are achieved and 2×2 LiNbO3 waveguide optical switch has been fabricated successfully. At the end of this paper, the losses of 2×2 LiNbO3 waveguide type optical switch are analyzed. The testing system is established. The V-grooves used for coupling have been designed.

我们在现有的实验条件下,经过反复实验,分析和改进,克服了光刻,溅射,扩散等各个阶段中的难题,总结出各部分最优的工艺参数,制备出了2×2定向耦合器型光波导开关,在本文的最后对制备出的光波导开关的各种损耗进行了分析,建立了测试系统,对光开关进行了通光测试,并对本2×2定向耦合器型光波导开关以后的耦合封装V型槽进行了设计。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。

A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。