进行中的

Purpose To explore the value of single cell isolation from tissue slides and single cell polymerase chain reaction in the study of lymphy node germinal centers.

目的探讨从组织切片中进行单细胞分离和单细胞聚合酶链反应技术在淋巴结生发中心研究中的应用。

Coumarin in melilotus was extracted in the method of acid-alkali, and the content of coumarin in product was too low and its configuration was changed in evidence.

采用碱溶酸沉法对草木樨中的香豆素进行提取，其提取物中香豆素含量过低，并且结构明显发生了改变。

It is composed of 4 chapters. Chapter 5 analyzes the farmers" behavior. It explains the character and the influencing factors of the farmers" behavior Using the Von Newman Morgenstern Utility Model to analyze to the individual, it could be concluded that the farmers AI behavior are influenced by many factors and it need joint work to ensure the farmer to attend the AI.

第五章对农民的行为进行了经济学分析，运用了经济学中的基本假设，分析了农业保险中农民行为的特点和影响农民行为的因素，通过冯·诺曼—摩根斯顿效用模型，对单个农民进行模拟分析后得出的理论结果与现实农民的行为不一致，从而得出结论：农民的农业保险行为受多种因素的影响，要保证农民的投保，是需要多方共同努力才能完成的。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

Fungal elicitors were extracted from Colletorichum lagnarinm, Phoma filtrate, Fusarium oxysponum, Asperillus niger and culture with HRPG. The total ginseng sponin and four kinds of monomeric sponins were analysed by UV spectrophotometry and RP HPLC.

提取黄瓜炭疽病菌 Colletorichumlagnarinm 、青菜炭疽病菌 Phomafiltrate 、棉花枯萎病菌 Fusariumoxysponum 、黑曲霉 Asperillusniger 中的真菌诱导子与人参毛状根共培养，应用HPLC及比色法对培养物中总苷及几种单体皂苷进行分析测定。

CFD-fluent simulation software and measurement are applied to establish the math models of exhaust gas concentration distribution and boundary conditions. Finite volume methods and PISO are used to solve the differential equations of exhaust gas distribution in a kitchen. After the adjustment and comparison with those from the practical measure, the simulation results of CO2 and CO concentration distributions accord with experimental ones.

运用CFD-fluent模拟软件和实测相结合的方法，建立废气的浓度和扩散分布的数学模型与边界条件，利用数值分析法中的有限体积法和PISO算法对厨房内废气分布的微分方程进行离散和求解，对模拟结果进行校正并与实际测试结果进行比较。

In situ hybridization using biotinylated POMC cRNA probe made it possible to localize POMC mRNA in the rat ovary. POMC mRNA predominantly existed in corpora lutea of diesturs, pregnant and pseudopregnant rat ovaries. POMC containing cells scattered in the ovarian intertitium were seen. The amount of POMC mRNA in granulosa cells was negligible.

采用生物素标记的POMCcRNA作为探针，进行原位杂文，研究POMCmRNA在大鼠卵巢中的分布，实验表明，POMCmRNA主要分布于间期，妊娠和假孕大鼠卵巢黄体中，间质细胞也可见少量POMCmRNA，而卵泡颗粒细胞中POMCmRNA极少。

Methods: TLC was used to identify Radix Scutellariae, Cortex moutan, Ramulus cinnamomi, Radix glycyrrhizae, and HPLC to determinate baicalin content was determined by HPLC.

对方中的黄芩、牡丹皮、桂枝、甘草建立TLC鉴别；用高效液相色谱法对方中黄芩苷进行含量测定。

If, upon such liquidation, dissolution or winding up of the affairs of the Company, the assets of the Company distributable among the Holders of Series A Convertible Preferred Stock and any other series of Preferred Stock ranking on a parity therewith in respect thereto or any class or series of capital stock of the Company ranking on a parity therewith in respect thereto shall be insufficient to permit the payment in full to all such Holders of shares of the preferential amounts payable to them, then the entire assets of the Company available for distribution to such Holders of shares shall be distributed ratably among such Holders in proportion to the respective amounts that would be payable per share if such assets were sufficient to permit payment in full.

公司资产中有可用于在A系列可转换优先股，以及与A系列股等价的其他相关系列优先股或其他相关种类或系列的公司股本股东之间进行分配的部分，在公司事务清算、解散或停业时，如果这一部分资产不足以对上述持有优先可支付额的股东进行全额支付，那么公司应调用全部可用资产，如果这些资产足够进行全额支付，则应根据此类股东各自的每股派息额数量按比例分配。

Therefore, the acceleration damp in concrete, the lateral and longitudinal propagation law on the acceleration in concrete, and relationship between acceleration and slip forming quality were tested and analysed theoretically and regressively.

本文因此就滑模摊铺过程中加速度在混凝土中的衰减、横向和纵向传播规律以及与成型质量关系进行了试验、理论分析和统计回归分析。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。