进行中的

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

As a differential detection is used between the intercarrier, the proposed algorithm overcome the high sensitivity to timing error of conventional algorithm and is robust to multi-path. The effect of sample frequency offset can't be ignored in a continuous OFDM system of a large number of subcarriers. The estimation precision of the conventional algorithm is low and the realization is complex. A joint estimation of sample frequency offset and fine frequency offset based on difference operation is proposed. As averaging use a large number of samples in a maximum likelihood algorithm, the estimation precision is very high. The use of difference operation cancel the effect of carrier frequency offset, and the proposed sampling frequency estimation algorithm is robust to carrier frequency offset.

最后，本文对连续系统中的采样频率和载波频率精同步技术进行较为深入的研究，由于子载波数目大的连续OFDM传输系统中，采样频率偏差不可忽视，以往的采样频率估计方法精度低、实现复杂，因此本文提出了一种基于差分处理的采样频率偏差和精载波频率偏差联合估计算法，利用了最大似然算法进行采样频率偏差的估计，由于采用了差分处理方法，消除了载波频率偏差对采样频率偏差估计的影响，采用的最大似然算法对大量样点进行平均化处理，所以，相对以往算法，提出的联合算法估计精度高，而且采样频率偏差估计对载波频率偏差具有鲁棒性。

Through designing of composition and structure of the bioactive graded coating,innerstress and its distribution in the coating were analyzed and calculated, the resultsshowed that when composition distribution coefficient n was 1.5, a reasonable stressdistribution could be got, that was at the beginning of deposition the suspension containingrichly BG granules was used so that a rich BG granules layer, a good transitional layerbetween BG layer at the bottom and the coating could be obtained at the titanium alloy side,the bottom of the coating; the stress value near the interface and surface and its character,pressure stress or tensile stress, were decided by the character of its composition itself.Changing composition distribution coefficient n could only change the variation tendency ofstress in the coating, but did not change the stress distribution rule in the coating. Thethinner the coating is, the sharper stress variation in the coating is, which does not mean thatthicker coating is better because the thicker the coating is, the little the permitteddeformation of coating is, so the coating thickness should be thinner, for example, about50μm for bending applications, but for applications only bearing pure shear stress, such asroot of tooth implant, the coating can be thicker little, for instance, about 80～100μm. The study on electrification characteristic and electrophoresis deposition of HAand BG granules in aqueous and non-aqueous solution system found that EPD almost didn'toccur in aqueous solution system. However, because HA granules take position charges inabsolute alcohol, a homogeneous EPD be carried out on the cathode titanium alloy slice, but taking negative charge in absolute alcohol the BG granules not be deposited on the cathode. A guided HA crystallizing, 100～300nm, on surface of the BG granules be realized by metathetical reaction, which cover BG granules with HA microcrystals and make the covered BG granules taking position charges in absolute alcohol, sequentially realize the EPCD of the BG and HA granules on the cathode, so it is feasible to make a titanium alloy/BG/HA bioactive graded coating by making use of EPCD technology. The corrosion experiment of rich boron bioglass coating and plasma spray coating showed that split phase, rich boron and rich silicon phase, occurred during its preparation. In basic medium the corrosion behavior of 〓 BG coating showed uniformity corrosion, the corrosion mostly occurred at rich boron phase area, therefore batch formula design of BGshould avoid the occurring of split phase. The corrosion appearance of plasma spray coatingappeared a non-uniform corrosion, mostly occurred at the edge of the laminated HA moltendrops, and emerged an accelerated corrosion tendency, which will easyly lead to corrosioncrackles extending to the interface and the happening of osmotic interfacial corrosion, thatmay be one of the major reasons leading to the coating cracking-off in the later period. Thetesting results of thermal expansion coefficient of 〓 and 〓BG showed the thermalexpansion coefficient of 〓 BG matched with that of titanium alloy better, and 〓 BG couldsinter with titanium alloy into densification enamel layer at low temperature (720℃).

将Ti6Al4V合金在1000℃下进行真空热处理会降低其力学性能，且合金内的V元素会向表面富集，因此，钛合金真空热处理和表面涂层的烧结温度不能过高，即应低于其相转变点；通过对生物活性梯度涂层的组成和结构的设计，分析和计算了梯度涂层内的应力大小和分布，结果表明：对于本研究，当成分分布系数n=1.5时，可以获得较合理的涂层力学性能，即在沉积开始时，采用富含BG颗粒的悬浮液，以便在钛合金侧获得同底层BG有良好过渡的富BG涂层；梯度涂层界面和表面的应力大小、性质由材料组成本身的性质决定，改变成分分布系数，只能改变涂层内应力变化的趋势；涂层的厚薄不影响涂层内的应力分布规律，但涂层越薄，涂层内的应力变化越快，但这并不意味着涂层越厚越好，因为涂层越厚，涂层允许的变形越小，对于应用于弯曲受力部位的涂层而言，涂层应薄一点为好(50μm)；而对于仅纯受剪切应力的部位，如牙根种植体，涂层可适当加厚(80～100μm)；通过对HA和BG颗粒在水溶液体系和非水溶液体系中的带电特性和电泳沉积的研究发现，它们在水溶液体系中很难发生电沉积；在无水乙醇溶液中，HA颗粒带正电，可在阴极钛合金片上发生均匀的电泳沉积，而BG颗粒则带负电荷；利用复分解反应法，可以制得100～300nm的HA，通过诱导HA在BG颗粒表面结晶，可对BG颗粒进行表面包覆，获得了被HA包覆的BG颗粒，改变了BG颗粒表面的带电特性，使BG和HA颗粒在无水乙醇中均带上正电荷，从而实现了HA和BG颗粒在阴极上的共沉积。

By use of titration analysis, the thesis investigated various factors such as isocyanate, PE molecular weight, reacting substance molecular ratio, reacting temperature and time etc. influencing upon prepolymerization step. It was found that 2, 4-tolulene diisocyanate and 4, 4'diphenylmethane diisocyanate is more rapidly reacted with PE600 than HDI. With PE molecular weight increasing, the reacting temperature or time should be raised or extended relevantly. Thus PE2000+2HDI pre-polymerization should be better carried out under some catalyst, for example, Triethylene diamine , Dibutyltin dilaurate and Tin octoate were used.

首先采用滴定分析方法对WRPU预聚反应的影响因素进行了研究：芳香族二异氰酸酯TDI、MDI比脂肪族二异氰酸酯HDI反应活性高，其中TDI在反应初始阶段的反应速率比MDI快，不过随着反应的进行，MDI比TDI反应速率加快，这主要是因为TDI分子中的两个-NCO反应活性差异大所致；二元醇分子量越大，反应速度越慢；聚醚二元醇比聚酯二元醇的反应速度快；当分子量超过2000的二元醇与HDI进行预聚反应时，需借助适当的催化剂来加快反应速度，从而使预聚反应能够顺利进行，分别选择DABCO、TOA和DBDTL三种催化剂进行催化反应，发现不同的催化剂，其催化效果相差较大。

Ethel ether, propanone and methyl alcohol were used for the separation of sample, BRUKER VECTOR33 FTIR spectrometer was used for test. The infrared spectrums are analyzed, the qualitative and quantitative analyses of the components are shown and the function of every composition is explained.

分别采用乙醚、丙酮、甲醇等有机溶剂对样品进行分离处理，通过BRUKER VECTOR33傅里叶变换红外光谱仪进行测试，对分离后各组份的红外光谱特征进行解析，给出样品的定性和定量分析结果；对各组份在清洗剂产品中的功能进行了分析说明。

CNTs were first pre-treated using acid solution (HNO3) to obtain CNTs functionalized with carboxylic groups. Subsequently, the stearyl alcohol was grafted onto CNT with the assistance of dehydrating agent, N,N'-dicyclohexyl-carbodiimide. Excellent dispersion in organic solvents such as acetone, THF, and chloroform was found for the modified CNTs (CNT-C18). In addition, the PBS/CNTs nanocomposites were then prepared through simple melt-blending. Mechanical properties, thermal behavior, conductivity of resultant polymer/CNT composites were investigated. The results show that excellent dispersion of nanotubes in the PBS matrices was achieved.

为了增加多壁奈米碳管在复合材料中的分散性，将碳管表面进行化学修饰，首先将多壁奈米碳管浸入HNO3溶液中，进行酸化，使碳管表面带有羧酸的官能基，然后再加入十八烷醇，在温和的条件下，藉由N，N'-二环己基碳二亚胺(N，N'-dicyclohexyl- carbodiimide，DCC)脱水剂脱水，使碳管和十八烷醇之间产生酯基的化学键结，改质后的碳管可以分散在acetone、THF、chloroform…等有机溶剂中，结果显示成功的利用DCC脱水剂完成碳管的改质。

The authors do not recommend rectal biopsy in all patients, but they note that "these group differences are important, as they suggest that different types of IBS may have different pathogenic mechanisms. By defining different 'key players' such as mast cells, lymphocytes, and EC cells, they suggest new specific pharmacological targets for therapy. They also suggest new objective measures such as plasma serotonin or serum tryptase with which we can seek to subclassify IBS and to identify those individuals who will respond to specific therapies."

作者并未建议所有患者进行直肠的组织切片检查，但是他们提到，这些组的差异是相当重要的，因为他们建议不同类型的 IBS可能有不同的病理机制，透过定义不同的关键如肥胖细胞、淋巴细胞和 EC 细胞，他们建议针对不同次族群以专一性疗法进行治疗，他们也建议我们以血浆中的血清素或血清中的胰蛋白酵素将IBS分类，如此可以将患者客观分类，进行专门的治疗。

Thist paper chooses 90 groups of "Tong_su_yi_xu synonymic words" from "Modern Chinese dictionary ", and divides them into three kinds according to the interpretation method in the dictionary, regards web page of Sohu website as the platform, us.es the search engine to carry on the search of the word , count the frequency that every group"s word appears , and rejects artificially the mistakes appearing in all web pages that can be opened, language fact presented in observation post then carefully (namely the example sentence with this word appearing in the webpage), and find that the 90 groups of "Tong_su__yi_xu synonymic words" except that the meaning of a word is the same, have quite a few of at frequency of utilization and concrete usage for difference: Because people"s use habit and word use the difference on to cause the appearance frequency of the word analogous to or with a wide gap, because of word-building ability, the word collocates, the word overlaps or the language usually overlaps, linguistic context of area and times , the meaning of a word that the word appears are abstract and concrete, semanteme is laid particular emphasis on, the morphological feature-change, etc.

本文从《现代汉语词典》中选取90组同素异序同义词，按词典中的释义方法将其分成三类，以搜狐网站的网页为平台，运用搜索引擎进行词语的搜索，统计下每组词语出现的频率，并将所有能打开的网页中出现的错误进行人工干预，然后仔细观察所出现的语言事实(即网页中出现的含有该词语的例句)，发现这90组同素异序同义词除了词义相同之外，有相当一部分在使用频率和具体的用法上还存在着差别：由于人们的使用习惯和词语使用上的差异造成词语的出现频率相当或者相差悬殊，又因为构词能力、词语搭配、词语重叠或语素重叠、词语出现的区域和年代语境、词义抽象与具体、语义侧重、词性变化等方面的差异造成词语使用上的区别。

Energy deposition at the interface of the particles caused by micro-explosive welding in explosive consolidation of metal powders is analyzed by symmetrical impaction model of uncompressible liquid. Two-dimensional equation of energy in the flow field of explosive welding is deduced and solved by finite difference method. Then the distribution of the temperature in the flow field is calculated on the basis of considering the adiabatic compression.

摘 要：以不可压缩理想流体对称碰撞模型对金属粉末爆炸烧结中由微爆炸焊接引起的颗粒界面附近沉积的能量进行了分析，在考虑热传导效应的基础上推导出了焊接流场中二维形式的能量方程并用有限差分法对其进行了求解，在考虑冲击绝热压缩贡献的基础上计算出了焊接流场中的温度分布。

Five different selective culture medium were used for sewage microorganisms directional isolated cultivate, advantage compound bacteria of photosynthetic bacteria, ammonifying bacteria, nitrifying bacteria, denitrifying bacteria and phosphate solubilizing bacteria could be gained through enrichment cultivate, then compounded them and used in test wastewater static treatment.

采用5种选择性培养基对水样中的微生物进行定向分离，通过富集培养，可以得到光合细菌、氨化菌、硝化菌、反硝化菌和磷细菌的优势菌群，进行复合后，再对试验污水进行静态处理。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。