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In order to cutting down the cost, flocculation of recombined Erwinia carotovora-Eccl3B cell from broth, influence of temperature, pH and oxidants on the separation of PHB were studied; and the method which measure the molecular weight of PHB by intrinsic viscosity was also studied; furthermore, a phenomenon was found that insolubility of PHB in chloroform can be changed by pretreated at over 120℃ for the fist time, and the mechanism of this phenomenon was explored.

为了降低成本,提高生产效率,本文针对发酵法生产PHB过程中存在的,发酵液难以分离沉淀,非有机溶剂提取PHB时分子量严重降低等问题,进行了发酵液的絮凝,及温度、pH、氧化剂对提取PHB影响的研究。并对粘度法测定PHB分子量的方法进行了研究,同时,对于研究过程中发现的,加热后PHB在三氯甲烷中的溶解性变化及其原因进行了探索。

Under the guidance of DPRIF integrating strategy, we set up an integreted SOFM-SVM model. Then we analyzed and optimized the model from the aspects of operating mechanism, data interface and function expandedness. The PCA method was introduced to reduce dimensionality and extract features, then to strengthen the clustering explanation; defined a CMI index, to ascertain the most effective or the best clustering number; A new Anti-NO algorithm was proposed to recognize and to filter the suspecious data in the sample; The medium result of SVM model was used to extract the borderline datas between two classified groups. This research compensated for the achievements of data recognition including pattern data, noises data and borderline data.

在DPRIF整合策略指导下构建了一个整合的SOFM-SVM模型,对该模型从运作机制、数据接口、功能扩展几个方面进行分析和改进:引入PCA方法进行数据降维和特征提取,用以加强聚类解释;结合统计聚类中的聚类误差概念定义一个聚类数有效性指标,利用SOFM算法中间结果进行指标求解,以筛选出有效或最佳聚类数;提出一种新的噪声识别算法用于对样本中的异常数据进行甄别和过滤;利用SVM模型的中间结果提取分类边界数据;进一步充实了包括模式类、噪声集和边界在内的模式识别成果。

The first part is the introduction, which generally introduces the research purpose and significance of this thesis, the summary of the financial pre-warning researches both home and abroad, and the research main contexts and research methods; The second part is the comparison on the financial pre-warning methods existent, which compares the different methods by categories, makes a further analysis on the limits of existent methods, and proposes the improve thoughts of this research based on that; The third part is the basic theory, which makes a summary of the referent theories exerted on the research, and exposit the comparing mechanism of the grey prediction theory and the artificial neural network method concise; The fourth part is the frame work of the model, which illuminates the research designs step by step, such as the definition of the financial crisis, the method of sample select, the select and definition of the pre-warning variables, and makes an exposition of the establishment procedures of the whole models at last; The fifth part is the financial pre-warning based on the theory of grey prediction and artificial neural network, which mainly demonstrate the model establishment in the concrete, dividing into four part to carry out the part of pragmatic research integrally, building of indexes system, establishment of BP neural networks, dynamic financial pre-warning and test, analysis on the pre-warning result; The sixth part is conclusion, which sums up the main results of the whole research, clarifies the innovative points, and makes a discussion on the limits and future study directions of the research in this thesis.

其中,第一部分为导论,总括性概述本文的研究目的与意义,国内外研究综述,以及本文的研究内容和方法;第二部分为现有财务预警研究方法剖析,通过对现有预警方法进行分类比较,进一步分析现有预警方法的局限性,并在此基础上提出本文研究的改进思路;第三部分基本理论,对本文研究中运用的相关理论进行扼要概述,并简要阐述灰色预测理论与人工神经方法的融合机理;第四部分为模型研究框架,逐步阐释模型对财务困境的界定,样本数据的选取方法,预警变量的选取与定义等各个部分的研究设计,并对整体模型的构建步骤进行简要阐述;第五部分为基于灰色神经网络模型的财务预警,主要论述动态预警模型的具体实现过程,分模型指标体系的建立,BP神经网络的建立,动态财务预警与检验,以及预警结果分析四个部分全面阐述本文实证分析过程;第六部分为结论,总结本文研究得到的主要研究结论,以及本文研究中的创新之处,并对论文的不足与后续发展研究进行探讨。

Pragmatic presupposition determines the choice of information focus and its position in a sentence,organizes the discourse into a coherent whole,concise,clear and smooth,and achieves the expected stylistic effects.in college english teaching,if teachers teach the knowledge about pragmatic presupposition in listening teaching,introduce the frequently-seen patterns of presupposition, increase the presuppositional sensitivity of students to certain words and structures,guide students to use the theory on presuppositional information to make forecasts on base of written listening material,listen purposefully,and get rid of the distraction of the minor and redundant information,the ability of students to obtain major information and linguistic inference will be strengthened and their listening competence will improve remarkably.george yule divided presuppositional triggers into factive presupposition,non-factive presupposition,counter-factive presupposition,existential presupposition,lexical presupposition and structural presupposition.presuppositional triggers are helpful for us to understand the true intention of the speaker or writer.in college english reading teaching,if teachers introduce the discourse organizing function of pragmatic presupposition and the relevant presupposition triggers,and teach students to use these triggers to communicate with the writer,the comprehension competence of students will be greatly enhanced.the essential function of presupposition is to build a framework for the further development of discourse.in college english writing teaching,teachers can use the theory on pragmatic presupposition to construct a handy training program for textual organization,increase the textual coherence, economy and structural perfection of the english writings by students.in college english translation teaching,teachers should focus on developing the pragmatic presuppositional thinking of students,enable them to grasp the presupposed information in source text / speech exactly,understand the original thoroughly,correctly reorganize and reproduce the pragmatic presuppositions in the target language,carry out the functional equivalence in translation and successfully realize the smooth and accurate communication.with the instruction of pragmatic presupposition in college english teaching,the complete understanding and integral acquisition of english and chinese of students will come true.in conclusion,to implement the curriculum requirements for college english teaching promulgated by the ministry of education of china,we should emphasize the instruction of the theory on pragmatic presupposition in college english teaching,carry it through from beginning to end.it's one of the important means to realize the college english teaching reform, improve the effects of college english teaching,strengthenr the practical and integrative language abilities of students,in order to cultivate the compound talents to adapt to the global integration and meet the requirements of the reform and opening in china.

在大学英语教学中,教师将语用预设知识贯穿于听力教学过程中,讲授一些常出现的预设类型,提高学生对一些特定的词语和结构的预设敏感度,引导学生运用预设信息理论对听力文字材料进行预测,有的放矢,排除次要、冗余信息的干扰,增强获取主要信息的能力和语言推理能力,从而提。。。更多高学生的听力理解水平。george yule将预设触发语分为事实预设、非事实预设、反事实预设、存在预设、词汇预设和结构预设,触发语有助于我们在阅读当中理解作者的真正用意。在大学英语阅读教学中,介绍语用预设的语篇组织功能和有关的预设触发语,并利用触发语来与作者进行交流,会极大地提高阅读理解能力。预设的基本功能就是为语篇的进一步发展建立一个框架。利用语用预设理论可以为大学英语写作教学构建一个易于操作的篇章组织训练方法,提高学生英语写作的语篇连贯性,经济性和结构优化。在大学英语翻译教学中,注重培养学生的语用预设思维,准确把握源语的前提信息,透彻理解原文,使语用预设在目的语中正确重组和再现,实现翻译的功能对等,交际的流畅性和准确性,并增强学生对英汉双语的全面性认识和整体性习得。综上所述,为更好地贯彻和实施教育部颁布的《大学英语课程教学要求》,在大学英语教学中重视语用预设理论的传授,使语用预设贯穿于大学英语教学的始终,是实现大学英语教学改革的重要手段,改善大学英语教学效果,增强学生的实用综合语言能力,培养符合全球一体化趋势,适应改革开放要求的复合型人才。

In coal gasification techniques, the gasifier is the key equipment which directly affects gasification characteristics, therefore flameless oxidant technology was applied to the design of new gasifier for high ash melting coal, and gasification characteristics in the gasifier were carried out by not only experiments under cold state and therma1 state, but also numerical simulations in this paper.

由于气化炉是煤气化技术中的最关键设备,因此,本文以高灰熔点煤的高效气化为背景,归纳分析了各种先进干煤粉气流床气化炉的炉型结构,将无焰氧化技术应用于粉煤气化,提出了一种基于无焰氧化的新型干法进料煤粉气化炉,进行了炉内冷态、煤粉气化的热态试验和数值计算,分析了高灰熔点煤在该气化炉中的气化特性,并以此为基础,对气化炉的结构进行了优化,提出了无焰氧化分级气化炉,对高灰熔点煤在该气化炉中气化特性进行了数值计算与分析。

In this paper, the 3-D finite element relocating mesh method is used to calculate the temperature field and the thermal creep stress field of spillway dam and mid level outlet dam in certain roller compacted concrete gravity dam, sensitivity analysis have been down with different parameters, such as placing temperature, the location of cooling pipe, cooling temperature, time for water supply, ascended-layer of concrete, intermissive time and different placing time, and the commendatory construction scheme have been put forward in such analysis; according by the test result on the two scheme of concrete aggregate combination (basalt as coarse aggregate and limestone as fine aggregate, basalt as coarse aggregate and basalt as fine aggregate), simulation analysis on temperature field and thermal creep stress field of the commendatory construction scheme have been down, and analysis have also been down on temperature field and thermal stress field of the two different aggregate combination, the result indicates that the spillway dam and mid level outlet dam which adopted these two aggregate combination can both satisfy the require of design crack resistance in concrete, furthermore limestone as fine is better than basalt as fine aggregate; the effect of summer water storage and winter water storage on the temperature field and thermal stress field have been analyzed, comparing with winter water storage, in summer water storage scheme the maximum tensile stress at abnormal concrete in upstream fill is decreased by 0.1~0.3MPa and there are no evidence change of the maximum tensile stress at other parts, which indicate that summer water storage is avail for the crack resistance of upstream fill; according to the simulation calculating result and comparing temperature simulation calculating results and temperature controlling measures of some similarity projects both completed and construction, synthesis appraise have been carried to the design temperature controlling measures of dam, and the temperature controlling measures of this project have been brought forward, all these have great reference value on the design of temperature controlling measures and the optimization of the construction scheme.

本文采用三维有限元浮动网格法仿真计算程序,对某碾压混凝土重力坝的溢流坝段和中孔坝段分别进行了三维有限元温度场和温度徐变应力仿真计算分析,对浇筑温度、冷却水管的布置方式、冷却水温、通水时间、浇筑升程和间歇时间、不同开始浇筑日期等参数进行了敏感性分析,在此基础上提出了推荐的施工方案;根据两种骨料方案(玄武岩粗骨料+灰岩细骨料方案;玄武岩粗骨料+玄武岩细骨料方案)的混凝土性能试验研究成果,对推荐的施工方案进行了温度场和温度徐变应力仿真计算,并对比分析了两种不同骨料情况下的温度场和温度应力,结果表明溢流坝段及中孔坝段采用上述两种骨料均能满足混凝土的抗裂设计要求,且灰岩细骨料优于玄武岩细骨料;计算分析了夏季蓄水和冬季蓄水对坝体温度场和温度应力的影响,夏季蓄水方案与冬季蓄水方案相比,坝体上游变态混凝土区域最大拉应力小0.1~0.3MPa,其他部位最大拉应力无明显变化,夏季蓄水对坝体上游面混凝土的抗裂有利;根据仿真计算成果并通过比较国内一些已建和在建的类似工程的温度仿真计算成果及采取的温控措施,对大坝的设计温控措施进行了综合评价,并提出了该工程的温控措施,对温控设计和优化施工方案具有重要参考价值。

Innovation achievements of this dissertation are mainly in the content and the method, namely: presents the content of EAP thoroughly and systematically; reviews the cost, utility and empirical studies of EAP; investigates the cost and utility of EAP theoretically, conducts the typical survey of the EAP implementation of a domestic enterprise; establishes multivariate linear regression equation of EAP utility; sets up an allocation model of EAP cost through Analytic Hierarchy ProcessThis dissertation\'s conclusion is that EAP cost is related to the time and frequency of EAP used by employee under the fixed cost, the tendency of using EAP is related to demographic characteristics and other factors, the utility of EAP is related to the personal preference of using EAP at least, the utility of EAP is leastwise related to 7 factors, the relationship with the supervisor is the most important factor in the workplace, personal health is the largest part of EAP cost.

本文的创新成果主要是内容上的创新和方法上的创新:对EAP的内容进行了全面、系统的梳理;对EAP的成本、效用和实证研究进行了综述;对EAP的成本、效用进行了理论上的探讨;对国内企业实施EAP的情况进行了典型调查;建立了EAP效用的多元线性回归方程;用层次分析法建立了EAP的成本分摊模型。本文得出的结论是:在固定成本不变的情况下,EAP的成本与员工使用EAP的时间及次数有关系;EAP的使用倾向与人口统计学特征和其它因素有关;EAP的效用至少与个人对EAP的使用偏好有关;EAP的效用至少与7个因素相关;与上级的关系是工作场所中最重要的因素;个人的身体健康是EAP成本中花费最大的部分。

Of Fe , Cu in the metal phase and Mg, Si in the silicate phases to understand the processes of mixing of metal and silicate phases and further decipher the mechanism of formation of mesosiderite;(2) to study in detail the geochemical characteristics of each phase in the meteorite, to provide necessaryevidence for the model of formation of mesosiderite;(3) to study and update in detail the composition of each phase in the meteorite, and provide a new and complete set of data for understanding the mechanism of formation of mesosiderite.

Cu和硅酸盐相中的Mg, Si等中等挥发性元素的稳定同位素进行分析,判断中铁陨石的金属相和硅酸盐相混合过程的环境特征,进而阐明中铁陨石的形成机制;(2)对这块陨石的各相进行详尽的地球化学分析,通过这些相的地球化学关系判断中铁陨石的形成机制,对中铁陨石的形成模型提供必要的制约和证据;(3)全面更新对中铁陨石各矿物相化学成份的认识,为中铁陨石的成因之谜的全面揭开提供最新最完整的数据。

Oil film thickness along the piston skirt in motored engine was measured, and the comparison with theory result was given.

最后在实验研究中,本文国内首次在活塞裙部油膜厚度中采用了激光荧光诱导的测试方法,主要工作是:分析了激光荧光诱导法的基本原理,建立了测膜厚的基本定量关系模型,通过实验分析了膜厚测试的各种影响因素,通过实验对不同香豆素荧光光谱和激发光谱特性进行了分析,通过实验确定了选取香豆素的种类和溶液浓度的基本原则,自主开发了以光纤为光学光路的测试系统并对系统进行了标定,对内燃机冷拖动状态的活塞裙部进行了实测,并与理论测试结果进行了对比。

In the practice of new rural community plan- ning and design for Badaling new village project, the au- thors think that farmers should always be the users and beneficiary, so that all planning should take farmers as the core, take rural development as a goal and regard farmers benefit as starting point. They point out the new rural com- munity planning should make comprehensive consideration around rural economic industry, land intensification, social structure, productive activity, ecological environment a...

就如何进行新农村社区的规划设计,笔者在实践中进行了总结,提出了应以农民为核心,以农民利益为出发点,以促进农村发展为目标,始终贯彻农民是使用者、受益者的思想;指出新农村社区规划应从农村经济产业、土地集约、社会结构、生产活动与生态、人文环境等多方面进行综合思考,并在空间规划中逐项落实的设计思路,介绍了在北京延庆八达岭镇的新村规划中所做的努力。

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推荐网络例句

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。