进行中

The study on the phylogenetic relations among these species is very useful for the study on genome and marker-assisted breeding in Brassica species. The karyotypic analysis is one of the most important methods in analysing the phylogenetic relation of species with closer relations. The protocol for inducing high frequency metaphase was established by pretreatment with low temperature and 8hydroxyquinoline, and then the preparation of chromosome and karyotypes analysis of six economical important Brassica species were carried out. According to the karyotypic analysis, it was shown that in the genomes of amphidiploid species, there are genomical components from didiploid species. Our result showed that B. campetris, B. juncea and B. napus belonged to class 2B, while B.

以二倍体B.nigra（B，n=8）基因组为探针，用B.campestris（A，n=10）或B.oleracea（C，n=9）的基因组进行封阻，分别与B.juncea（AB，n=18）和B.carinata（BC，n=17）进行GISH杂交发现，B.juncea和B.carinata基因组中仅有来自于B.nigra的8对染色体表现杂交信号，而其它染色体杂交信号较弱；以B.campestris或B.oleracea基因组为杂交探针与B.napus（AC，n=19）进行GISH杂交发现，所有19对染色体均出现杂交信号，根据杂交结果难以正确判断B.napus基因组中的哪些染色体是来自于A基因组，哪些是来自于C基因组；以rRNA基因（18S-5.8S-26S）为探针的FISH杂交结果表明，B.campestris基因组中rRNA基因位点为6对；B.oleracea为3对；B.nigra为2对；B.carinata为4对；B.juncea为6对；B.napus为6对。

Equidistance point and difference theory in theory of function approximation are studied. Meanwhile, the relation among difference, difference quotient and derivate is revealed. By drawing Lagrange's and Cauchy's theorem of mean on difference and Taylor's formula into difference function, four theorems, such as Lagrange's theorem of mean on difference, are concluded in simple way. On the basis of these conclusions, the asymptotic property of middle point is studied, a series of new conclusions are drawn and the discussions on the asymptotic property of middle point in differential mid-value are summarized.

对函数逼近论中等距节点和差分理论进行了研究，揭示了差分、差商与导数之间的联系；将Lagrange中值定理、Cauchy中值定理、Taylor公式引入到差分函数中，简明地推导出Lagrange差分中值定理等4个定理，并在此基础上对"中间点"的渐近性进行了研究，得出了一系列"中间点"的渐近性的结果，概括了有关文献对微分中值公式的"中间点"的渐近性的讨论；给出的引理改进了函数逼近论的证明方法，精简了函数逼近论中的一些内容。

Chapter 4, lists the teaching cases in teacher's teaching, combined the cases dissecting the teaching methods of mathematics and mathematical cerebration and methods in the point of view of psychology, then infers that to proceed the teaching of mathematical cerebration and methods is necessary. In the other hand, I compared and studied the association between the basic mathematical cerebration and methods and other mathematical knowledge, answer the questions that which mathematical cerebration and methods are more worthy to jut out. At last, infers a mode of the teaching in mathematical cerebration and methods.

第四章列举了教师在授课中的教学案例，结合案例从心理学角度对中学数学教学与数学思想方法教学进行剖析，得出进行数学思想方法教学是十分必要的；同时对中学数学教学中的数学基本思想方法与其它数学知识的关系进行了对比分析，对在中学数学教学中突出哪些数学基本思想方法为好做了解答；最后给出了数学思想方法教学的一个教学模式。

First of all, confirm the overall evaluating indicators and the base indicators of public transport network in Chinese large cities through repetitive comparison, discussion and consultation to relevant experts; among the base indicators in the overall evaluating indicators, composite the basic probability assignment of evaluation indicators with Dempster-Shafer Theory composition rule upon different experts' experiences and preferences of the expert group, and amalgamate the evaluations of different experts to further obtain the evaluation value of base indicators.

首先通过反复比较、探讨和征询有关专家的意见，确定大城市公交线网综合评价指标及子指标，在综合评价指标的子指标中，对专家组中不同专家的经验和偏好，用证据理论合成规则对评价指标的基本概率赋值进行合成，将不同专家的评价进行融合，获得子指标的评价值，然后通过突变理论将评价指标及子指标作为控制变量进行地面公交线网的综合评价，对北京市进行算例分析结果表明，该方法对大城市公交线网的综合评价是可行。

First scanning the known handwriting materials then number them ,In pretreatment, we convert the valid part of the image into a standard size, as images carries out duotone and go throw off chirp handling in order to achieve better effect.draw features from the known handwriting materials with the Co-occurence,especially,we divided a copy of handwriting into 25 little pieces with the size of 128*128 ,drawing features from every little piecese with four directions(0 degree, 45 degrees, 90 degrees as well as 135 degrees) and calculate the four major feature values( veins and the statistical quantity of veins contrast and the statistical quantity of veins consistency Shang the statistical quantity of statistical quantity as well as the veins correlation of gray scale ), preservation all the feature value that drawn from all known ma terials to the handwriting characteristic database,then input the unknown handwriting materials, also using the method of the Co-occurence to draw those features, recycling the minimum European Distance law match the unknown writing material feature value with the handwriting characteristic feature database, export the label of the known hand writing materials which is most similar to the unknown material with minimum European Distance, and then we can confirm who is the author of the unknown material.

首先将笔迹材料作为图象扫描输入，并对其进行编号。预处理部分可将笔迹图象的有效部分规范化到一个统一尺寸，接着对其进行二值化和去除噪声的处理，以便于更好的提取图像的特征。在此我们采用了灰度共生矩阵法提取手写笔迹材料的纹理特征，与以往有所不同的是，我们将一份手写材料分割成64块大小为80*80象素的子图象，每个小块都从四个方向（0度、45度、90度以及135度）来更全面的提取特征，并计算出四个最主要的特征值（纹理一致性的统计量、纹理反差的统计量、纹理熵的统计量以及纹理灰度相关性的统计量），将从所有已知材料提取的特征值保存到纹理特征库中，对于待检手写材料，同样采用灰度共生矩阵的方法提取其纹理特征，再利用最小欧氏距离分类法将从待检手写材料中提取的特征值与纹理特征库中的特征值进行比对，与欧氏距离比对值最小的相匹配，输出匹配成功的原材料的标号，进而识别出待检材料书写者的身份。

The protein and phospholipid constituent in membrane of neurocyte in brain of chicken with encephalomalacia was separated and studied with the method of SDS-PAGE electrophoresis and the method of high performance-thin layer chromatography respectively The fluidity of neurocyte membrane of chicken with encephalomalacia was determined for the first time The content of Ca and trace elements were determined in chicken encephalomalacia systematically and the status of Fe ion in cerebellum was observed. The activity of enzymes that were relative to metabolism of energy and neuro-medium in brain were checked systematically with the method of enzyme-histochemistry. Pathological examination was carried out by microscopic and ultramicroscopic observation. The apoptosis of neurocyte in brain was checked.

在进行临床观察、定期剖杀并检测体内V〓含量、体重和脑重的基础上，重点研究了与神经损伤有关的以下几个方面的内容：系统地检测了体内自由基代谢和抗氧化系统功能的变化；首次采用SDS-PAGE电泳法分离并检测了雏鸡脑软化症神经细胞膜蛋白组分，并以高效薄层层析法检测神经细胞膜磷脂组分；测定了脑软化症雏鸡神经细胞膜的膜流动性；首次系统检测了脑软化症雏鸡体内钙及微量元素含量，并进行了小脑组织总铁离子状态观察；采用酶组织化学方法系统地观察了脑组织中与脑能量代谢和神经递质有关的酶的活性；对相关组织进行了病理组织学和超微结构观察；并对脑组织中神经细胞的凋亡进行了检测。

The vapour and plasma ignition times of the target are got based on the equation of heat conduction and cascade model. We suppose that the plasma is ignited when 210 generations of new electrons are born in vapour generation time. The influence of temperature on the thermodynamics and optics parameters of material also have been considered in the model. The resulting theory has good agreement with the experiment and overseas report. A blade method to measure the laser spot is given in this paper with validation and error analysis. The plasma threshold of metals in atmosphere and water ambients both are diagnosed with the light deflection and piezoelectric transducer. A Q-switched pulsed Nd:YAG laser operating at infrared (1064nm), visible(532nm) and ultraviolet (355nm) wavelengths has been used. Al、Fe、Cu are used as targets and get a similar results with both method. Theoretical and experimental analyses are applied on the influence of wavelength on the threshold have been done, both of which have shown that the plasma threshold of metals decrease as the laser wavelength increases; The plasma threshold of metals are higher in water than in air and the pressure of the shock wave in water is five times higher than in air.

从热传导方程和雪崩电离机制出发，假设当电子增值2~(10)倍时，考虑了温度对材料热力学和光学参数的影响时，得到了气化和等离子体点燃的时间，利用该模型进行计算得到的结果与国内外报道及自行通过实验测得的阈值基本一致；提出了利用刀刃法测量激光光斑面积的方法，并通过实验进行了验证和误差分析；利用光偏转装置和压电换能器分别对空气和水中金属等离子体点燃阈值进行了实验诊断，激光器均为调Q—YAG激光器(波长1064nm，532nm，355nm，脉宽10ns)，靶材分别为Al、Fe和Cu，两种测试方法得到的等离子体点燃阈值基本一致；本文从实验和理论计算两个方面讨论了波长对等离子体点燃阈值的影响，均得到了等离子体点燃阈值随着波长的增加而减小的结论；对空气中和水中不同环境下金属等离子体的点燃阈值进行了比较研究，得到了金属在水中的等离子体点燃阈值比空气中的大，且水中产生的冲击波的压强是空气中的5倍左右的结论。

Seed explants of Eleutherococcus senticosus produced somatic embryos directly ongrowth regulator-free medium. The results were showed that use low temperature stressmethod to relieve the Eleutherococcus senticosus seed conformation dormancy andphysiological dormancy, to induce seed germinate. Proliferation of Eleutherococcus senticosussomatic embryo without any plant growth regulathors was better in liquid medium containing1/3MS+sucrose 3%, and better in solid medium containing 1/3MS+sucrose 1%.Eleutherococcus senticosus secondary embryo was stick to matrix through espial of cytology.Eleutherococcus senticosus somatic embryo was cultivated some times to form immature frondin liquid medium, then transfer into solid medium to form full plant. The experiment wasindicated that much more Eleutherococcus senticosus immature frond can be cultured inbioreactor, so large-scale production in bioreactor culture can be done.

研究结果表明：低温胁迫处理刺五加种子可以打破种子的形态休眠和生理休眠，促使种子发芽：在不添加任何植物激素的培养基中诱导刺五加体胚形成，其中，1／3MS+3％蔗糖的培养基搭配有利于液体培养的刺五加体胚增值，1／3MS+1.0％蔗糖的培养基搭配有利于固体培养的刺五加体胚增值；在进行体细胞胚增殖时，体细胞胚继代三周后表面开始形成很多次生胚，对刺五加次生胚进行细胞学观察，发现刺五加次生胚与母体只是粘贴在一起；刺五加体胚需在液体培养基中培养一段时间形成幼植物体，实验表明可以通过生物反应器培养大量的刺五加幼植物体，然后转入固体培养基中形成完整植株，因此可以通过生物反应器培养进行规模化生产。

Firstly, a probe into the dual implications of burden of proof and the expurgation of its intrinsic meaning and result meaning is burden of proof in applied conditions of burden of proof .We should make a definition of applied conditions of burden of proof .The thesis makes a research into the internal structure of applied conditions of burden of proof so as to clearly show the triple contents of its internal structure. In addition, the thesis makes a probe into its regulating mechanism. It points out clearly that applied conditions of burden of proof must ensure the correct application and cautious application of burden of proof.

首先，通过对证明责任的双重含义进行考辨，廓清证明责任的两层含义中本质意义抑或结果意义乃是我所说的证明责任适用条件中的证明责任，对于证明责任适用条件的含义进行界定；继而，文章对证明责任适用条件的内部构造进行研究，指明其内部构造的三重内容；进而文章对证明责任适用条件的约束机制进行了探讨，明确指出，证明责任适用条件必须能够保障证明责任的正确适用与谨慎适用。

It is the first important step in video analysis and will directly affects the effectiveness of indexing Shot boundary detection is one of our major research interests and we will tackle the following existing problems the ambiguity between gradual change and camera motion, the discontinuity during gradual change, false detection caused by illumination variation and flashlight, automatic threshold selection Firstly, we discuss shot boundary detection in non-compressed domain In chapter 2, we compare some of the commonly used detection methods which are based on frame difference and point out that single feature will not generate good results As a conclusion, we use fuzzy logic to combine multiple features Presently, most frame difference based shot boundary detection algorithms rely on threshold and hence the selection of such thresholds will greatly affect the performance of boundary detection We propose a membership function to define frame difference and calculate the membership with self adaptation according to the statistic distribution of frame differences to satisfy different type of video clips Experiments show that the proposed fuzzy shot boundary detection algorithm can be used with different video types and has a high detection precision and recall In chapter 3, we discuss model-based shot boundary detection algorithms regarding chromatic and spatial editing effects such as fade-in, fade-out, dissolve and wipe Various parameters are proposed to better describe the characteristics of each editing type.

镜头边界的检测是把视频自动地分割为一个个镜头，作为基本的索引单元，因此它是视频分析重要的第一步，直接影响到视频检索的成败。镜头边界的检测是本文研究的重点之一。目前镜头边界检测算法主要存在以下问题：渐变与镜头运动难以区别；渐变过程中的不连续与停顿、光照条件的变化及闪光灯等特殊情况会引起误检测；自动选择阈值比较困难等。本文首先针对非压缩域视频进行了镜头边界检测的研究。在第二章中我们采用了比较流行的基于帧间差的方法。在比较各种帧间差计算方法的基础上，指出使用单一的特征难以取得很好的检测效果，提出用模糊逻辑综合使用各种特征。目前大多数基于帧间差的镜头边界检测算法都采用阈值法进行镜头转换的判别。阈值选择的误差对检测性能有较大的影响，本文提出用隶属度函数定义帧间差较大、中等较大和较小等概念，并根据帧间差的统计分布自适应地确定隶属度，以适应不同类型的视频片断。实验结果表明这种基于糊逻辑的镜头边界检测算法可以适应不同的视频，并具有较高的检测精度和检出率。在第三章中采用基于模型的方法进行镜头渐变的检测，研究了淡入／淡出、慢转换和扫换的模型。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。