进入位点

There are three copies of R fragment in the S mt-genome, and each copy contains two closely adjacent open reading frames: orf355 and orf77. Among these three copies two BamHI fragments were cloned, and the sequence analysis showed that cox1 and cox2 gene lies upstream of the R region in each fragment, respectively. However, they locate in different strands and head to head with R. In this study, we found that:(1) The expression of cox1 and cox2 is not affected by the transcription of R region;(2) The two copies of R region transcribe in large amount in the microspores, and produce transcripts of 1.6kb and 2.8kb, respectively, in the sterile microspores; However, the abundance of these two transcripts is reduced in the fertility restored microspores, realized through the mRNA decay, and this reduction occurs at the uninucleate microspore stage;(3) In the sterile microspores, the 5 terminus of the 1.6kb transcript contains a palindrome region that can fold into a stem-loop.

本研究发现：(1) cox1和cox2的转录没有受到R区转录的影响；(2)两个拷贝的R区在在不育花粉中高丰度表达，其转录本大小分别为1.6kb和2.8kb；而在育性恢复的花粉中，这两个转录本的丰度被大大降低，进一步分析表明这一结果是通过mRNA分子的降解途径来实现的，此降解过程发生在单核花粉期；(3)在不育花粉中，1.6kb转录本的5′末端具有一段可形成茎环结构的迴文对称序列，而在可育花粉中，其5′末端短缺了9个碱基；(4)在不育花粉和育性恢复的花粉中，R区转录本的加尾位点都集中位于一个3′茎环结构之后；(5)不管是在不育花粉还是育性恢复的花粉中，orf77区域内均在第52位和100位核苷酸处发生了不同频率的C向U的编辑，从而形成UGA终止密码；(6)不管是orf55-orf77的成熟转录本还是转录前体分子，都已被加上了Poly尾巴，表明已进入降解途径；在以上实验结果的基础上，本研究对S型CMS花粉育性的恢复机理进行了如下推测：在不育花粉和育性恢复的花粉中，orf77区域内均发生了终止编辑，即由RNA编辑产生了提前终止密码子。

Further study of the interaction between NodD and naringenin by FRET technology is underway.

通过对naringenin分子上六个不同位点，不同性质，长度连接链以及母体和荧光团之间两种连接方式的11个荧光标记物的合成以及生物活性评价，我们找到了一个荧光标记物，它保留了野生型naringenin约20％的活性，并将其用于根瘤菌固氮过程的研究，发现该荧光标记物能够顺利进入根瘤菌活细胞中，为下一步通过FRET技术研究naringenin和NodD蛋白之间的相互作用打下了基础。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了