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还原酶

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In order to understand the mechanism of anti-inflammatory effect of PA and carvacrol, the superoxide dismutase, glutahoine reductase, glutahoine peroxidase activities in liver and malondialdehyde , cyclooxygenase 2 (COX-2), tumor necrosis factor-α levels were eveluated. Also observed inducible nitric oxide synthase and cyclooxygenase-2 (COX-2) expression and the pathological histology in λ-carrageenan induced paw edema in mice.

本研究首先以醋酸扭体试验评估左手香水抽提物及其活性成分香芹酚是否具有镇痛效果,以福马林舔足试验辨别其镇痛作用是在周边或是中枢,再以λ-角叉菜胶诱导小鼠足跖肿胀试验探讨其抗发炎作用,并分析其肝脏组织中的超氧歧化酶、麸胱甘肽还原酶、麸胱甘肽过氧化酶活性、发炎足跖组织中的脂质过氧化指标产物丙二醛、肿瘤坏死因子-α及环氧化酶-2之含量变化,观察诱导型一氧化氮合成酶及环氧化酶-2之蛋白质表现量,并将肿胀足跖做病理切片观察之,以探讨左手香水抽提物及香芹酚之抗发炎机转。

They were named respectively as muramidase-released protein; succinate dehydrogenase/fumarate reductase flavoprotein subunit (SDHFp/FRDFP, extracellular); bacterial trigger factor; elongation factor G (EF-G, extracellular); PTS system sorbose subfamily I (PTS/MAN-ⅡAB, cytoplasmic); pyruvate kinase; phosphofructokinase, PK.

经MALDI-TOF-MS鉴定,这8个蛋白分别为溶菌酶释放蛋白、琥珀脱氢酶/延胡索酸还原酶黄素蛋白亚单位(SDHFp/FRDFp)、触发因子、延长因子G、细菌糖磷酸转移酶系统甘露糖特异ⅡAB亚单位、丙酮酸激酶、6-磷酸果糖激酶(6-PFK)、色氨酸-tRNA合成酶。

After 24 hours, all rats were anesthetized again and their left common carotid arteries were cannulated to facilitate blood withdrawal for serum sample. The contents of tumor necrosis factor-a and interleukin-6 (IL-6) in serum were determined before and after experiment by ELISA; the activity of superoxide dismutase and content of malondialdehyde were determined by xanthinoxidanse method and modified TBA microdetermination. The content of NO2(superscript -)/NO3(superscript -), and activity of nitric oxide synthase and inducible NOS were determined by nitrate reductase method, and chemical chromogenic reaction, respectively; the immunoglobulin G, IgA, IgM and complement 3 (C3) and C4 were determined by immunoprecipitation method before and after experiment.

实验评估:手术创伤后24h进行采用酶联免疫吸附法测定实验前后血清肿瘤坏死因子α、白细胞介素6含量;应用黄嘌呤氧化酶法测定超氧化物歧化酶活性、改良TBA微量法测定丙二醛水平;以硝酸还原酶法、化学显色法分别检测NO2/NO3浓度及一氧化氮合酶、诱导型一氧化氮合酶活胜;以免疫比浊法经全自动生化分析仪检测血清免疫球蛋白IgG、IgM、IgA及补体C3、C4含量。

The oversaturation of cholesterol in bile results in the formation of cholesterol stones. The liver is the organ which synthesizes cholesterol and secretes bile. Liver lipid metabolism research helps to explain the mechanism of cholelithiasis. Utilizing biochemical and biomolecular technology, we have done experimental work as follows: Analyse the ingredient changes of blood and bile samples from patients.

肝脏是合成胆固醇、分泌胆汁酸的器官,对肝脏胆固醇代谢的研究有助于阐明胆石症形成的原因,本文用生物化学和分子生物学的方法,分析了病人血液、胆汁中各种脂类成份的变化,以及肝脏胆固醇合成限速酶3-羟-3-甲戊二酸辅酶A还原酶(HMG-CoA Reductase)、胆汁酸合成限速酶7α-羟化酶(7α-hydrolase)的基因表达。

Androgen ablation therapy with leuprorelin acetate caused a decrease in the expression of both 5α-reductases. Our data indicate that different type of 5α-reductase is involved in the pathogenesis of BPH and PCA.

根据我们的结果可以认为在良性前列腺增生中以Ⅱ型还原酶的作用为主而Ⅰ型还原酶则可能在前列腺癌的发生中起主要作用。

NiRs are divided into two different types based on their prosthetic groups, namely heme-containing nitrite reductases (cd1-NiRs) and Copper-containing nitrite reductases.

根据辅基的不同, NiRs分为血红素cd1型亚硝酸还原酶(cd1-NiRs)和铜型亚硝酸还原酶两种。

Selenium-dependent (glutathione peroxidases and thioredoxin reductases ) and selenium-independent (superoxide dismutases and catalase ) enzyme systems regulate cellular ROS steady state leels.

硒依赖性酶(谷胱苷肽过氧化物酶和硫氧还蛋白还原酶及非硒依赖性酶(超氧化物歧化酶和过氧化氢酶)系统共同调节使细胞内ROS处于稳态。

Northern blotting analysis indicated that 5 genes, nitrate transporter gene, nitrite reductase gene, ammonium transporter gene, ATP-binding cassette transporter gene, and purine permease gene, were significantly up-regulated under nitrogen starvation.

Northern blotting验证其中5个基因,包括硝酸盐转运蛋白基因、铁氧化还原蛋白亚硝酸还原酶基因、铵盐转运蛋白基因、结合ATP盒的转运蛋白基因和嘌呤透过酶基因,在缺氮诱导条件下转录水平有明显上调。

The amount ofethylene release by shoot apex and root showed no difference between iron-deficient treatment and control plants above before the appearance ofrelatively high reductase activity,which indicated that ethylene may not playa direct role in regulation of root ferric reductase activity,and the inhibitionof which by〓and〓may be caused by other reasons.

缺铁处理植株茎尖和根系乙烯释放量的测定结果显示,三种植物中,缺铁与对照植株的茎尖及根系所释放的乙烯可能不直接参与缺铁根系还原酶的调控,〓和〓对缺铁根系〓还原酶活性的抑制可能是其它原因所致。

It is Investigated that the effects of vitamin C and E on M. nipponense in different molting stages by measuring oxygen consumption rates, ammonia-N excretion rates and activities of antioxidant enzymes(SOD,CAT,GSH-Px,GST,GR) in three groups which are control group,Vc group andgroup.1. The oxygen consumption rates are high in stages D2 and A, but low in stage C.

设立三个试验组:对照组,V_C组(200mgV_C/kg)和V_C+V_E组(200mgV_C/kg+400mg V_E/kg),以上述分期结果为标准,测定了三个试验组的日本沼虾在蜕皮各时期的耗氧率,排氨率以及抗氧化酶(超氧化物歧化酶SOD,过氧化氢酶CAT,谷胱甘肽过氧化物酶GSH-P_X,谷胱甘肽转移酶GST,谷胱甘肽还原酶GR)活性的变化,结果如下: 1。

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