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还原酶

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The results show that all the hmgr genes from pigs, cattle and chickens are astable enzyme proteins with the same gene sequence characteristics and encoding protein and physical and chemical properties; all the amino acid sequences have a high degree of homology, containing two conservative HMG-CoA binding motifs and two NADPH binding motifs; all the amino acid sequences, containing signal peptide, are secreted proteins; they are all hydrophobic transmembrane proteins with five transmembrane domains; random coil and α-helix are the main structural elements in the HMGR polypeptide chain; all the predictions of three-dimensional structure are V- shaped; there are more closer evolution relationship between the HMGR gene from pigs and cattle.

结果表明:猪、牛和鸡HMGR基因序列特点及编码蛋白质理化性质基本相同,均属不稳定类酶蛋白;保守结构域氨基酸序列具有高度的同源性,均含有保守的2个β-羟基-β-甲基戊二酸单酰辅酶A结合基序和2个还原性烟酰胺腺嘌呤二核苷酸结合基序;均有信号肽,属分泌型蛋白质;属于跨膜的疏水性蛋白,均有5个跨膜结构域;无规则卷曲和α-螺旋是HMGR多肽链中的主要结构元件;三维结构预测都呈"V"字形;猪和牛HMGR基因的进化关系最近。

In the presence of cellobiose, CDH can also reduce the products derived from guaiacol oxodation by peroxidases and therefore prevent the polymerization of these products.

在纤维二糖存在的条件下,纤维二糖脱氢酶可以还原过氧化物酶氧化愈创木酚形成的产物,并抑制产物的聚合反应。

Mmol/L. The activity of this catalase was not reduced by dithionite but was strongly inhibited by cyanide, azide, and 3-amino-1,2,4-triazole (the specific inhibitor of monofunctional catalase).

该过氧化氢酶不受连二亚硫酸钠的还原作用影响,但被氰化物、叠氮化物和3-氨基-1,2,4-三唑(单功能过氧化氢酶的专一抑制剂)强烈抑制。

Methods The DNA injury was determined with single cell gel electrophoresis, the contents of NO and NOS were measured by NO and NOS kits respectively and apoptosis of lymphocytes was determined by flow cytometry and gelose electrophoresis.

建立硫芥中毒淋巴细胞模型,分别用单细胞凝胶电泳法检测淋巴细胞DNA损伤;用镀铜镉还原法和一氧化氮合成酶试剂盒检测中毒后淋巴细胞培养上清液一氧化氮和一氧化氮合酶的变化;用流式细胞仪和琼脂糖凝胶电泳法检测细胞凋亡。

hydrogenase and hydrogenase are the main types of hydrogenase s and have different s.

氢酶是产氢过程中的关键酶,催化氢的氧化或质子的还原。

According to the metal atoms within hydrogenase , it can be classified as NiFe- hydrogenase , Fe- hydrogenase and metal-free hydrogenase .

氢酶是一类催化氢的氧化或质子还原的酶,它在微生物产氢过程中扮演着重要角色。

Raw enzyme was extracted after the bacteria were ultrasonically broken, and its 2-oxoalde-hyde reductase activity was detected by HPLC and UV absorbency spectrum in the presence of NADH and methylglyoxal as a substrate.

破碎该菌细胞后提取粗酶,以甲基乙二醛作反应底物,在还原性辅酶Ⅰ存在条件下,紫外光谱和HPLC的分析表明,该菌能代谢甲基乙二醛;并对其产酶条件进行了研究。

Thirty-one new chiral phosphorus compounds have been synthesized starting from D-camphor,-alpha-phenylethylamine,-Betti base,-1-(4-nitrophenyl)-2-amino- propane-1,3-diol, L-amino acids (such as serine, phenylalanine, proline etc.) and their application as organocatalysts in asymmetric trimethylsilylcyanation of aldehydes and asymmetric borane reduction of prochiral ketones and ketimines were investigated.

该项目从D-樟脑、-alpha-苯乙胺、-Betti碱、-氯酶胺、L-氨基酸(如丝氨酸、苯丙氨酸、脯氨酸等)出发合成了31个新的手性磷化合物,研究了它们作为有机催化剂在醛的不对称硅氰化反应、潜手性酮的不对称硼烷还原反应以及亚胺的不对称硼烷还原反应中的催化效果。

Finally, a suggested refolding was separately presented for the reduced and non-reducing egg white lysozymes in the urea solution.

最后,给出了一个建议的还原和非还原蛋白溶菌酶在脲溶液中的重折叠过程。

The refolding of reduced and non-reducing egg white lysozymes in a urea solution was studied by a "phase diagram" method of fluorescence.

用荧光&相图&法研究了还原和非还原脲变性蛋白溶菌酶的重折叠。

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