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还原酶

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In order to optimize enzymolysis technology for production of antioxidant peptides from chickpea protein, effect of ratio of enzyme to substrate, enzymolysis temperature, and hydrolysis time on the technology in which reducibility and superoxide anion radical capturing rate were taken as response values was analyzed with response surface methodology.

为优化Alcalase蛋白酶酶解鹰嘴豆蛋白制备抗氧化肽的工艺条件,采用响应面分析法,以还原能力、超氧阴离子捕获率为响应值,研究了酶与底物的比值、酶解温度和酶解时间对制备抗氧化肽工艺的影响。

Ratio of enzyme to substrate, temperature, and hydrolysis time for the production of antioxidant peptides were analyzed with Response surface methodology in which reducibility and superoxide anion radical capturing rate was response value to build a predictive model for the enzymolysis of chickpea protein and the optimization of the enzymolysis technology parameters.

为优化Alcalase蛋白酶酶解鹰嘴豆蛋白制备抗氧化肽的工艺条件,采用响应面分析法(response surface methodology,简称RSM),以还原能力、超氧阴离子捕获率为响应值,研究了酶与底物的比值、酶解温度和酶解时间对制备抗氧化肽工艺的影响。

Sequencing revealed that the most common genes appearedin the library is photorespiratory-related genes, such as ribulose bisphosphate carboxylase, Rubisco activase, glyoxylate aminotransferase, glutamine synthetase,glyceraldehyde dehydrogenase, ferredoxin, transport protein, etc.

测序之后发现最为显著的是一些光呼吸相关的基因如核酮糖-1,5-二磷酸羧化/加氧酶、Rubisco激活酶、乙醛酸转氨酶、谷氨酸转氨酶、甘油醛脱氢酶、铁氧还原蛋白和转运蛋白等。

Five kinds of bacteria were isolated from disease Meretrix meretrix and culture water (two isolated from the M. meretrix, three from the culture water) in Lvsi harbor, Jiangsu province. The results of infection showed that strain WG1702 can cause disease with the mortality rate 100 percent and the same symptoms as original. It was cued that strain WG1702 was the pathogenic bacteria and caused the death in large scare, a preliminary research was done on the bacteria including morphological, physiological, biochemical characteristics, phylogenetic position and pathogenic. Strain WG1702 was a straight-rod gram negative bacterium, the physiological and biochemical indicators were lysine decarboxylase, ornithine decarboxylase, reduction of NO3- to NO2-, mannitose, oxidase and methylred reaction positive, arginine decarboxylase, arginine dihydrolase, salicin and voges-proskauer negative.

本文从江苏吕泗文蛤养殖区发病文蛤和养殖水体中分离到5株优势菌(病蛤中分离到3株优势菌,养殖水体中分离到2株优势菌),人工感染实验结果显示,菌株WG1702能引起供试文蛤发病,死亡率为100%,且发病症状与原发病症状相同,提示该菌是引起文蛤大面积死亡的主要致病菌,对其形态、生理生化特征、分类地位及致病性进行了初步研究,菌株WG1702为革兰氏阴性菌,直杆状,生理生化指标为:赖氨酸脱羧酶、鸟氨酸脱羧酶、硝酸盐还原、甘露糖、氧化酶、甲基红阳性,精氨酸脱羧酶、精氨酸双水解酶、水杨素、V.P阴性。

AOX branches from the cytochrome pathway at the level of the ubiquinone and catalyses the oxidation of ubiquinol and reduction of oxygen to water.

从主呼吸链的辅酶Q分岔,是氧化辅酶Q、还原氧分子生成水的另一终端氧化酶。

Deamidation of the milk protein produced the spicy or ammonia-like flavor.Reduction and heat treatment had no obvious effect on the enzyme reaction and the flavor of the product.The order of different enzymes could influence the flavor effectively.In addition,the taste of the hydrolysate can not be improved by adding condiment.

结果表明,脱酰胺反应会使产品具有氨味和辛辣味;还原处理和加热处理,对脱脂乳粉和乳清浓缩蛋白的酶解速度和水解液风味都无明显影响;不同的酶解顺序对乳蛋白水解液的风味影响显著;用风味剂不能有效地掩盖酶解液的不良风味。

Taking Shewanella and Geobacter as examples, this paper reviewed three kinds of possible mechanisms of microbial enzyme-catalyzed dissimilatory Fe reduction and its molecular regulation, i.e., direct contact between microbial cell and Fe oxides, siderrophores, and electron shuttling compounds. Many membrane-bound cytochrome proteins are involved in the dissimilatory Fe reduction, forming a complicated regulation network.

以希瓦氏菌和地杆菌为例,本文综述了3种异化Fe还原的酶促反应机制及其分子调控机理:异化Fe还原菌与Fe氧化物直接接触机制、电子穿梭体的作用机制、铁载体作用机制,多种膜蛋白特别是多血红素的细胞色素蛋白参与微生物的异化Fe还原过程,并形成复杂的调控网络。

By the partial purification of Arthrobacter nicotinovorus D-97 endocellular enzymes and the results of enzymatic reaction of enzymes of partition collection, it was found that enzymes related to trehalose synthesis were consisted of Trehalose-forming Enzyme and Trehalose-releasing Enzyme . Trehalose-forming Enzyme could convert maltooligosaccharides into non-reducing maltooligosyl trehalose by intramolecular transglycosylation, and Trehalose-releasing Enzyme could hydrolyze α-1, 4 linkage between the trehalose residue and the maltooligosaccharide residue to release trehalose and maltooligosaccharides with two glucose monomer lesser.

将食尼古丁节杆菌D-97胞内酶部分纯化并对各收集组分进行酶反应,发现其海藻糖合成酶系由海藻糖生成酶(Trehalose-forming Enzyme,TFE)和海藻糖释放酶(Trehalose-releasingEnzyme,TRE)组成,前者分子内转糖基将直链麦芽寡糖转化为没有还原性的麦芽寡糖基海藻糖,后者专一切割麦芽寡糖基海藻糖分子上麦芽寡糖基与海藻糖连接的α-1,4糖苷键,从而释放出海藻糖和减少了两个葡萄糖单体的麦芽寡糖。

The aggregation interaction between reduced-denatured egg white lysozymes during refolding procedure in urea solution was studied by means of reducing and non-reducing protein electrophoreses.

通过还原和非还原蛋白电泳,研究了还原变性蛋白溶菌酶在脲溶液中重折叠过程的集聚相互作用。

With the existence of additional carbon sources, sodium lactate, sodium propionate or sodium acetate, the faster pH rised, the faster p, p'-DDT dechlorinated, which indicate that the existence of short chain organic substrate supplies sufficient electron donors for the dechlorination process, and p, p'-DDT can obtain electrons more easily during the stage of pH rising.

并且由于p,p'-DDT的还原脱氯与细胞色素氧化酶及辅基FAD有关,外加碳源下p,p'-DDT的缺氧降解多数发生在体系pH值上升的阶段,且pH值上升较快时对应的p,p'-DDT缺氧降解较快,而在无外加碳源的体系中,由于可供p,p'-DDT还原脱氯的电子量限制了p,p'-DDT还原脱氯的速度,未发现上述现象。

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