还原酶

The function of the addition of co-substrate ethanol was to in-situ regenerate NADH. A mathematical model was derived, in which, two substrates were catalytically reacted in sequence. The model was applied to the system involving the reduction of 2'-chloroacetophenone to R-2'-chloro-1-phenyl-ethanol by S.

深入研究了在乙醇存在下生物还原过程中酵母细胞内NADH、NAD〓、NADPH及NADP〓浓度的变化规律，发现胞内NADPH浓度迅速降低到零，而NADH浓度始终维持在较高水平，证明了用于底物还原反应的辅酶是NADH，辅助底物乙醇的加入有利于实现辅酶Ⅰ的原位再生。

The metabolism of these extreme microbes during the production of Maotai Liquor would further produce multiple enzymes of thermal stability such as amylase, protease, saccharifying enzyme, cellulose, glucase, xylanase, and each kind of dehydrase involved in redox reaction, and DNA polyase etc.

茅台酒酿造过程中极端酿酒微生物代谢产生多种热稳定性的酶，如淀粉酶、蛋白酶、糖化酶、纤维素酶、葡萄糖甘酶、木聚糖酶、参与氧化还原反应的各种脱氢酶、磷酸烯醇丙酮酸激酶及DNA聚合酶等。

The identified proteins were divided into two following categories: one is regulatory proteins, for example, caffeic acid 3-O-methyltransferas, HSP70, glyoxalase I , actin, and so on. They are involved in protein biosynthesis and processing, cellular structural organization and stress responses. The other is function proteins, for instance, ascorbate peroxidase, cytoplasmic malate dehydrogenase, cytosolic phosphoglycerate kinase, S-adenosyl methionine synthetase, ATP synthase, and so on. They are involved in photosynthesis, photorespiration, redox homeostasis, and metabolisms.

通过分析可以将这些差异蛋白分为两大类：第一类是调节性蛋白，如咖啡酸三氧转甲基酶，热激蛋白70，醛酮变位酶I，肌动蛋白等，它们主要参与蛋白质的合成和加工、胁迫响应、细胞骨架稳定等过程；第二类是功能性蛋白，如抗坏血酸过氧化物酶，细胞质苹果酸脱氢酶，细胞质磷酸甘油酸激酶，S-腺苷蛋氨酸合成酶，ATP合酶等，它们则参与氧化还原平衡、光合作用、光呼吸和各种代谢等过程。

In this review, the latest progress about the involved main enzymes, their gene expression regulation, and the comparison of the dissimilatory nitrate reduction between fungi and bacteria were discussed.

此文从参与该过程的关键酶、关键酶的表达调节、真菌与细菌异化硝酸盐还原的比较等角度综述了真菌异化硝酸盐还原的最新研究进展。

This Thesis consists of the following five pacts: Following a brief Introduction on the mechanism of reactions of coenzyme NADH models with activated ethylenic compounds is discussed. Pross-Shaik treatment is employed to analyze the results.

本论文研究了：(1)辅酶NADH模型物和活化烯烃氧化还原反应的机理，并运用Pross-Shaik构型混合模型分析了这些反应的机理；(2)用辅酶NAD~+／NADH模型物催化氧化一级和二级苄醇，还原α，β-环氧酮。

Sodium lauroyl sarcosine was used as a denaturant, a novel protein refolding method, which was proposed on the basis of one step-AMC and IEC, one step-AMC-IEC was used on the refolding of denatured/reduced lysozyme.

一步人工分子伴侣-离子交换色谱法辅助还原变性溶菌酶的复性：以月桂酰基肌氨酸钠(Sodium Lauroyl Sarcosine，Sarkosyl，SLS)作为蛋白变性剂，发展了一步人工分子伴侣-离子交换色谱法，对还原变性溶菌酶进行了复性研究。

In deaerated buffer solutions, the cyclic voltammetry of the composite films of GOx-Au NP-CHIT showed a pair of well-behaved redox peaks that were assigned to the redox reaction of GOx, confirming the effective immobilization of GOx on the composite film.

研究表明：在除氧缓冲溶液中，葡萄糖氧化酶-壳聚糖-纳米金复合膜修饰电极表现出一对良好的氧化还原峰，这对峰归因于葡萄糖氧化酶的氧化还原，证明葡萄糖氧化酶被成功固载于复合膜内。

As a result,nitrate was deoxidized into nitrite.It provides a new way to determine the concentration of nitrate in vegetable.Revivification efficiency was chose as investigation index in the paper.

以还原效率为考察指标，在单因素实验的基础上，通过正交实验研究了酶法还原蔬菜中硝酸盐的最佳反应条件为pH值为7，反应温度30℃，酶浓度200 u/L，NADPH浓度4 mmol/L，反应时间30 min。

Results of non-reducing sodium dodecyl sulphate polyacrylamide gel electrophoresis of the supernatant and aggregate precipitate formed in refolding process show that except being refolded to native egg white lysozymes, the reduced-denatured lysozymes can also form the aggregates with molecular weights being separately about 30.0 and 35.0 kD, while the reducing SDS-PAGE and the refolding results in the presence of sodium dodecyl sulphate show that these aggregates are formed chiefly through the misconnection of disulfide bonds between the reduced-denatured lysozymes, and the aggregate precipitates are formed through the non-covalent interactions between the aggregates with molecular weight being about 30.0 kD.

复性过程形成的上清和集聚体沉淀的非还原电泳结果表明，除了能复性成天然态的溶菌酶分子外，还原变性蛋白溶菌酶同时还能形成分子量分别约为30.0KD和35.0KD的蛋白溶菌酶分子集聚体；而它们的还原电泳和在SDS存在时的复性结果表明，这些集聚体主要是通过还原变性蛋白溶菌酶分子间的二硫键错配而形成，而集聚体沉淀则是通过分子量约为30.0KD的集聚体分子之间的非共价相互作用而形成。

Chapter 3.3, In "Methods of Enzymatic Analysis", Vol.7, 3rd Ed., Ed. by Bermeyer, H.V., pp.

而met-Mb的蓄积与met-Mb还原酶活性呈负相关性(图 2及3)。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新