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AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞;以不同浓度的LPS(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml)和不同作用时间(0min,5min,15min,30min,60min,120min)分别刺激小室培养的细胞单层,观察NF-κB的核内浓度及NO合成量的动态变化,选择LPS的最佳用量和作用时间;然后分成四组实验,设正常对照组,LPS处理组,特异性PKC抑制剂阻断组,NF-κB抑制剂阻断组;收集培养的单层细胞及培养液;采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平;免疫组化法检测NF-κB的核内移位变化;硝酸还原酶法测定细胞培养液中NO含量;吖啶橙染色观察凋亡细胞的形态学变化,凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

Blue light could increase NRactivity,respirative rate,decreased the net photosyntheticrate,increased the contents of total nitrogen,protein,aminoacid,making the N metabolism stronger.

蓝光可以提高硝酸还原酶活性和呼吸速率,降低净光合速率,提高使叶片总氮、蛋白质、氨基酸含量提高,使氮代谢增强,C/N降低。

The nitrate reductase activity,chlorophyll concentration and nitrogen content of tobacco leaf and total absorption area of tobacco rhizotaxy were significantly or extremely sign ificantly correlated with nitrogen concentration.

结果表明,不同基因型烟草对氮素营养的响应有明显差异,其硝酸盐还原酶活性、叶绿素含量、根系总吸收面积、氮含量均与氮浓度呈显著或极显著相关。

The screening method of seven alleles (Z-6,Z-4,Z -2,Z,Z+2,Z+4 and Z+6) in then dinucleotide tandem repeat sequence was st udied. These alleles constitute a microsatellite DNA marker upstream of the tran scription initiation site on the aldose reductase gene.

湖南医科大学学报000103 摘要:研究了醛糖还原酶基因转录起始点上游-2.1kb处的微卫星DNA标记--n二核苷酸串联重复序列7种等位基因(Z+6,Z+4,Z+2,Z,Z-2,Z-4和Z-6)的筛选方法。

The screening method of seven alleles (Z-6,Z-4,Z -2,Z,Z+2,Z+4 and Z+6) in then dinucleotide tandem repeat sequence was st udied. These alleles constitute a microsatellite DNA marker upstream of the tran scription initiation site on the aldose reductase gene.

研究了醛糖还原酶基因转录起始点上游-2.1kb处的微卫星DNA标记--n二核苷酸串联重复序列7种等位基因(Z+6,Z+4,Z+2,Z,Z-2,Z-4和Z-6)的筛选方法。

Relationship between NO3--N Accumulation and Nitrate Reductase Activity in Mesophyll and Petiole of Different Spinach Spinacia oleacea L.

不同品种菠菜叶肉及叶柄中硝态氮累积与硝酸还原酶活性的关系植物生理学通讯第42卷第2期,2006年4月,217-220

The mechanism of nitrate accumulation in six spinach ( Spinacia oleacea L.) varieties under low nitrogen level was studied in a pot experiment.

提要 盆栽试验条件下,菠菜体内的硝态氮含量及硝酸还原酶活性因品种和部位而异。

Keywords : Pomegranate leaf tannide;HMG-CoA redutase;hyperlipidemic model

中文关键词:石榴叶鞣质;HMG-CoA还原酶;高脂模型

Objective To study relationship between serum and red blood cell folate, serum vitamin B12 (VB12), total plasma homocysteine, thermolabile genotypes, and activity of methylene-tetrahydrofolate reductase.

目的 探讨叶酸、维生素B12(VB12)、血浆总同型半胱氨酸、5,10-亚甲基四氢叶酸还原酶活性及其热敏感性基因型间的相互关系。

Thedegenerate primers were designed and synthesized according to the sequence of NDHB amino acid published in GneBank. A novel cDNA encoding the second subunit of NADH dehydrogenase was amplified from Fraxinus velutina Torr by RT-PCR.

根据GenBank上已发表的NDHB氨基酸序列,设计合成了一对简并引物,采用RT-PCR技术对绒毛白蜡NADH还原酶第二亚基基因进行扩增,将扩增的PCR产物与pMD18-T连接后转化至E。

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