还原酶

Abstract］objective: to detect the reduction of rabbit nadp-dependent retinal reductaseon menadione, and to identify its reductive production.

目的：检测兔辅酶ⅱ依赖性视黄醛还原酶对维生素k3的催化活性并对该酶还原k3所生成的产物进行鉴定。

Center for Molecular Biology, Shantou University Medical College, Shantou 515041, China［Abstract］Objective: To detect the reduction of rabbit NADP-dependent retinal reductaseon menadione, and to identify its reductive production.

目的：检测兔辅酶Ⅱ依赖性视黄醛还原酶对维生素K3的催化活性并对该酶还原K3所生成的产物进行鉴定。

The activities of glycerol dehydrogenase and 1,3-PD oxidoreductase were also studied, and it was found that the activity of GDHt was enhanced, but the mutation had little influence on the activity of PDOR.

同时测定了诱变前后还原途径中甘油脱水酶和1，3-丙二醇氧化还原酶的酶活变化，研究表明诱变对GDHt有明显的促进作用，而对PDOR的影响不明显。

The potential sources of NO in plants include nitric oxide synthase, nitrate reductase, xanthine oxidoreductase and the nonenzymatic pathway.

在植物中， NO的潜在来源包括一氧化氮合成酶、硝酸还原酶、黄嘌呤氧化还原酶和非酶促途径。

Four different catechin content cultivars were selected from our tea germplasm appraisal database to assay the gene expression level of the seven genes, flavonol synthase, dihydroflavonol-4-reductase, leucoanthocyanidin reductase, anthocyanidin synthase, etc, which were involved in the flavonoids biosynthesis. The result showed that, the DFR and LAR transcripts were expressed increased with the increasing of tea catechin contents. Nevertheless, the others did not show this tendency clearly.

利用半定量PCR技术检测总儿茶素含量不同的4个茶树品种中与类黄酮合成相关的黄酮醇合成酶、二氢黄酮醇－4-还原酶、无色花色素还原酶、花色素合成酶等7个基因的表达情况，结果表明DFR和LAR基因的表达量与茶树中总儿茶素含量呈一定的相关性，而其他基因则与其相关性不大。

The type Ⅰ and type Ⅱ mRNA levels were measured in 15 normal, 45 hyperplastic and 15 cancerous tissues.

为了进一步研究两型5α-还原酶同工酶在正常、良性增生及前列腺癌中的作用，我们应用RT-PCR方法对Ⅰ型及Ⅱ型还原酶mRNA在各种不同前列腺组织中的表达进行了定性及半定量研究。

NADP-Dependent Retinol Dehydrogenase/Reductase identified from rabbit liver cytosol is involved in oxidoreduction between retinol and retinal with high activity. Therefore, NRDR plays an important role in modulation of the retinoids and RA homeostasis.

辅酶Ⅱ依赖性视黄醇脱氢/还原酶NADP(H-Dependent Retinol Dehydrogenase /Reductase NRDR是黄东阳教授等从兔肝中发现并纯化的一种酶，具有很高的催化视黄醛和视黄醇之间氧化还原反应的活性，对体内类视黄醇和维甲酸的代谢平衡起着重要的调节作用。

The results of asymmetric reduction of latent chiral ketones by carbonyl reductase showed that the enzyme had a high specificity for pentanone in aliphatic ketones and for the acetophenone derivates that contained a withdrawing group at α-position.

利用羰基还原酶不对称催化还原潜手性酮类化合物发现，对于芳香酮类化合物，酶对α位为强电负性基团的底物专一性较强；对于脂肪酮类化合物，酶对五碳脂肪酮的专性较高。

Finally confirmed that,The thighbone and the osteoporosis morbidity is possibly connected protein 6, respectively are: The lactoferrin light chain, membrane association protein A3, the enolase, ATP gather the enzyme, the acetyl coenzyme A reductases, the myo calcium protein; The lumbar vertebra and the osteoporosis morbidity is possibly connected protein 5, respectively are: The actin, the keratin, the enolase, ATP gather the enzyme, the myosin; The thighbone and the strong bone valuable curative effect is possibly connected protein 9, respectively are: The enolase, ATP gather the enzyme, the myo-calcium protein, the creatine activating enzyme isozyme, the phosphoglyceric acid change flavor the enzyme, the myosin, the lactoferrin light chain, the pyruvic acid activating enzyme isozyme, the crown protein; The lumbar vertebra and the strong bone valuable curative effect are possibly connected protein 8, respectively are: The carbonic anhydrase, the actin, the αB-crystal protein, 3-phospho-glycerol aldehyde oxidase, the serum albumin, ATP gather the enzyme, the myosin, the enolase.

最后确认：股骨与骨质疏松发病可能相关的蛋白6个，分别为：乳铁蛋白轻链、膜联蛋白A3、烯醇化酶、ATP合酶、乙酰辅酶A还原酶、肌钙蛋白；腰椎与骨质疏松发病可能相关的蛋白5个，分别为：肌动蛋白、角蛋白、烯醇化酶、ATP合酶、肌球蛋白；股骨与强骨宝疗效可能相关的蛋白9个，分别为：烯醇化酶、ATP合酶、肌钙蛋白、肌酸激酶同工酶、磷酸甘油酸变味酶、肌球蛋白、乳铁蛋白轻链、丙酮酸激酶同工酶、冠蛋白；腰椎与强骨宝疗效可能相关的蛋白8个，分别为：碳酸酐酶、肌动蛋白、αB-晶体蛋白、3-磷酸甘油醛脱氢酶、血清白蛋白、ATP合酶、肌球蛋白、烯醇化酶。

Five expressed genes contributed to the flowering process and embryonic development such as prolamine, gene for allergenic protein, chalcone synthase, putative peptide methionine sulfoxide reductase and acyl carrier protein Ⅱ gene were chosen to hybridize with a 1510-RNA-blot (time-point phenotypes) array. The 1510 RNA blots were prepared into an RNA array for the purpose of conferring and validating global transcriptional profiles on booting, flowering and filling quantitatively and qualitatively at a statistic level. The results verified: Prolamine and allergenic protein were high-expressed in filling grains after being regulated by development, Chalcone synthase and methionine sulfoxide reductase were high-expressed in the leavies induced by light, in the root stressed for nitrogen deficiency.

为了全局性分析基因表达谱以及进一步验证它们与开花过程的相关性，选取参与水稻开花过程和胚胎发育过程的基因：查尔酮合酶、酰基载体蛋白Ⅱ、醇溶蛋白、S-腺苷基甲硫氨酸还原酶、过敏反应蛋白等为探针，与1500个水稻RNA斑点阵列进行杂交时，结果证实：醇溶蛋白和过敏反应蛋白受发育调节在乳熟成穗中高表达，查尔酮合酶和S-腺苷基甲硫氨酸还原酶在叶片中受光诱导、在根中受缺氮胁迫高表达。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。