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Part of test solution is abstracted and in hydrochloric acid, W is reduced to quintavalent W and producing yellow complex compound with patassium rhodanate by dual reducting agents of tin chloride and titanium oxide.

吸取部分试液,在盐酸介质中,以氯化亚锡—三氯化钛双还原剂将钨原成五价并与硫氰酸钾生成黄色络合物。

The silver nanoparticles were synthesized using sesbania gum at hot temperature and characterized with UV-visible absorption spectroscopy, Scanning electron microscopy and transmission electron microscopy.

本文使用绿色化学方法,选用天然高分子多聚糖田菁胶为还原剂和稳定剂,一步合成了一种具有环保特性的、稳定性好的银溶胶。

Sesbania Gum-silver nanoparticles was synthesized at room temperature using green chemistry method.

根据绿色化学法,室温条件下,使用田菁胶为还原剂和稳定剂合成银溶胶。

It was discussed in theory that the various equilibriums of stannous chloride affect on reduction potentials under the different PH val- ue solution with the experiment of orthogonal design.

并通过正交试验方法对还原剂的电势变化规律进行了理论探讨;着重探讨了在不同PH值条件下氯化亚锡溶液中存在的不同平衡状态对还原电势的影响。

Micrometer-sized MoO2 hollow spheres were synthesized hydrothermally with ammonium heptamolybdate tetrahydrate as molybdenum source, Cetyltrimethylammonium bromide as structure-directing agent and C2H5OH as reducing agent, respectively.

在阳离子表面活性剂的作用下,以钼酸铵为钼源,乙醇作为还原剂合成了微米级MoO2空心球。

Solid state reduction of raw titaniferous magnetic ore, its sinter , and pellets with H_2 and CO have been studied at various temperatures.

用CO和H_2为还原剂研究了钛磁铁矿原矿,烧結矿和球团矿的还原性。

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2~3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12,在酵母细胞中实现了多拷贝强表达;荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液,测定510 nm处的荧光发射强度结果显示,表达的绿色荧光蛋白对氧化还原水平敏感,且在510 nm处的荧光强度与一定的重金属浓度呈正相关,即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强,从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用;同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属;遗传学的点突变频率及致死率实验数据表明,重金属能导致菌体的点突变频率和致死率升高,且活性氧的清除剂巯基脲能明显降低这种点突变和致死率,说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

Results With an increase of the trisodium citrate, the AuNPs became smaller under TEM.

结果 透射电子显微镜显示随着还原剂柠檬酸钠加入量的增加,形成的纳米金的颗粒逐渐减小。

Methods The recombinant plasmid of CPB, pET-21a-CPB, was constructed and transformed into E. coli BL21(DE3). The expression was induced in 25℃and 37℃, respectively. The obtained inclusion bodies were dissolved in different denaturing solution systems. The protein concentration was determined in order to indicate the solubility of CPB inclusion bodies in the denaturing solution. And probable mechanism for the solubility differences was illuminated by the analysis of unreduced SDS-PAGE.

方法构建CPB重组质粒pET-21a-CPB,将其导入表达菌株BL21(DE3)中,分别在25 ℃和37℃进行诱导表达;所得包涵体分别用不同浓度尿素添加不同还原剂溶解,以溶液中的蛋白质浓度判定其溶解性,利用非还原SDS-PAGE分析其溶解性提高的原因。

And the Ni nanorods were prepared by reduction of NiCl2 with hydrazine in the virgulate liquid crystals microemulsion as colloidal chemical template at 70℃.

并在70℃时,以棒状液晶结构的微乳液为反应模板,用水合肼为还原剂还原NiCl2,合成出棒状纳米Ni。

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