运动神经元

Injury to the corticospinal tract, which has facilatory influence on motor neurons in the spinal cord, causes the "negative" manifestations including muscle weakness, fatigability, and decreased dexterity (Rossi, 1994). These features are the major determinant of motor disability associated with upper motor neuron lesions.

痉挛在极易影响运动神经元的脊髓灰质损伤中带来的阴性效应包括肌肉无力、易疲劳、灵巧性下降，这些特征成为与上运动神经元损伤并发的运动残疾的主要决定因素。

In conclusion, Schwann cells from wallerian degenerating facial nerves of rats might bear with similar biological characteristics and functional phenotype to those from sciatic nerves in vitro, and they might be able to produce soluble NTFs for trophic support on facial motoneuron cultures. The establishment of methods for obtaining high-purity facial motoneuron cultures of rats with partially labeled fluorescence should be of significance in contribution to further investigation on facial motoneuron's response to NTFs and other affecting factors.

综上所述，本文通过面神经雪旺氏细胞体外培养条件下生物学特性、功能表型，及其对培养面神经运动神经元细胞的营养活性作用的研究，为在体外条件下研究雪旺氏细胞对面神经运动神经元的营养支持作用时，如何选择细胞来源，提供了实验依据；建立了获得较高纯度并带荧光标记的面神经运动神经元的分离和体外培养方法，为进一步研究影响面神经生长、损伤后再生修复的因素奠定了实验基础。

RESULTS: The mild traction group showed angulus anterior individual neuron slightly engorged, neuron and nerve fiber morphous was normal essentially; individual substantia alba demyelinates in fragmentis; medullary sheath and auxiliary fibers of nerve root were hydropsia slightly without conspicuous myelinolysis. The moderate traction group demonstrated myelinolysis change in the spinal cord, and neurofibras lined up chaos, auxiliary fibers disaggregated, neuron dropsy, tigroid body disappeared with karyopycnosis and anachromasis in neurons; nerve root showed myelinolysis. The severe traction group displayed a great demyelination region; anterior motor neurons with karyopycnosis and anachromasis; myelinated nerve fiber of nerve root shrinked, decreased in number, with severe demyelination changes.

结果：轻度牵拉组牵拉侧前角个别神经元稍肿胀，神经元及神经纤维形态基本上正常，个别白质小片状脱髓鞘样改变，神经根髓鞘和轴索轻度水肿，脱髓鞘不明显；中度牵拉组白质有脱髓鞘改变，神经纤维排列紊乱，轴索崩解、断裂；灰质神经元水肿明显加重，前角运动神经原细胞尼氏体消失，核固缩、深染，神经根髓鞘和轴索水肿，局部轻度脱髓鞘改变；重度牵拉组牵拉侧白质为不规则的大片脱髓鞘区，前角运动神经元胞体固缩、变形、核深染，神经根有髓神经纤维萎缩、数目减少，重度脱髓鞘改变。

These results can lead to the following conclusion:〤GRP synthesized by the motoneurons may be a self-serving neurotrophic factor after axotomy.(2) CGRP may serve as a "injury signal" activating both the central and peripherical gli

本研究结果提示，运动神经轴突损伤后，运动神经元合成分泌的CGRP对神经元自身具有直接的神经营养作用，CGRP还可以作为损伤神经元分泌的&损伤信号&激活中枢和外周胶质细胞，从而为运动神经元的存活和轴突再生创造有利的微环境。

Methods Employing cholera toxin B-subunit combined horseradish peroxidase retrograde tracing technique and cholera toxin B-subunit combined colloid goldretrograde tracing with calcitonin gene-related peptideimmunohistochemical double-labeled staining technique,the innervation of musculus sacrococcygeus ventralis medialisin rats was studied.

大鼠骶尾腹内侧肌又称荐尾腹侧中肌(musculus sacrococcygeus ventralis medialis)或屈尾短肌，是位于盆腔后壁、骶椎前方的一对梭形肌肉，属尾肌群，因其功能似乎仅为屈尾，故至今尚未引起人们的注意，而与该肌位置关系较密切的会阴肌因在某些运动神经元疾患时仍可保持其正常生理功能而一直受到神经工作者的关注，尤其是近年来有诸多文献报道了会阴肌的神经支配、相关神经元的细胞构筑、神经递质和神经调质的分布以及纤维联系特点［1～10］，藉以探讨运动神经元疾病的神经病理学。

Get the neonate Sprague-Dawley rats medulla spinalis, through the conventional primary cell culture procedure, we use the method of difference adherence time and get the motor neurons of anterior spinal cord. When the neuron is in maturity, we make the mechanical injury model in vitro. All the models were divided into four groups: group A is control group; group B is 100μM ATP group; group C is 100μM ATP+20μg/ml suramin group and group D is 100μM ATP+10μM ouabain+10μg/ml Thapsigargin group. Culture the four groups neurons, after one day, we count the motor neuron, observe the survival and activity of neurons through MTT shade selection experiment, use flow cytometry to analyze the percentage of apoptosis of motor neurons of anterior spinal cord and detect the expression of protein p-GSK-3β(ser9) through Western-Blot technology.

方法取新生大鼠脊髓，通过常规的原代细胞培养程序，采用差速贴壁法分离出大鼠脊髓前角运动神经元，培养成熟后制作神经元机械损伤体外模型，分为A组：对照组、B组：100μMATP组、C组：100gMATP+20μg/ml Suramin组和D组：100μM ATP+IOμM Ouabain+10μg/ml Thapsigargin组，对各组机械损伤的运动神经元进行培养，1天后分别进行运动神经元计数、MTT比色实验观察运动神经元的存活及活性、流式细胞仪分析机械损伤的脊髓前角运动神经元凋亡百分率和Werstern Blot技术检测p-GSK-3β(ser9)的表达。

Results 45-51% of the nerve fibers innervating the superior rectus muscle in rats of group Ⅰ and 81-87% in group Ⅱ came from the neuron in contralateral midbrain, and the distribution of the neurons in midbrain was closer to normal rats in group Ⅱ than group Ⅰ.

结果 实验Ⅰ组大鼠支配上直肌的神经纤维有45%~51%由对侧中脑运动神经元发出；实验Ⅱ组81%~87%由对侧中脑运动神经元发出，神经元在中脑的分布更接近正常大鼠。

The number of spinal motoneurons continued to decline in the first 3 months postoperativdy and plateaued after three months.

脊髓前角运动神经元数目在3个月内持续减少，3个月后趋于稳定，6个月时脊髓前角大型运动细胞坏死比率在40％左右，残存的神经元多为受损的神经元，尼氏体减少或消失。

It was observed by Nissl and enzyme histochemistry staining that exogenous GDNF decreased lesion-induced motor neuron death in lateral nucleus of spinal anterior horn and the changes in activity of cholinesterase and acid phosphatase in spinal cord and sensory ganglions.

采用硅管套接大鼠切断的坐骨神经模型，局部给予胶质细胞源性神经营养因子，应用尼氏染色、酶组织化学染色方法，观察到外源性GDNF能减少脊髓修复侧前角运动神经元死亡的数目，降低脊髓前角运动神经元及脊神经节感觉神经元中胆碱酯酶及酸性磷酸酶变化的幅度。

"Sensory neurons relay information from sense organs, motor neurons carry impulse s to muscles and glands, and interneurons transmit impulse s between sensory and motor neurons."

感觉神经元传递自感觉器官处传来的讯息，运动神经元则传递神经冲动至肌肉和腺体处，而联络神经元则负责传递感觉神经元和运动神经元之间的神经冲动。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了