过高的

To compensate the descent of pH for the heat-up, exalting the Tris-HCl buffer's pH can avoid the abscission of the purine and make the long sequence PCR go on smoothly. The GC-rich template's secondary structure is complex and stable. The organic reagent methylamine contributes to uncoil the secondary structure.The pfu DNA polymerase has more fidelity and thermostability than Taq DNA polymerase.

高GC含量使模板二级结构复杂而稳定，一般热启动难于解开，而变性温度过高、时间过长又不利于DNA聚合酶的活性作用发挥，于是借助能缓释模板二级结构的有机试剂甲胺扩增高GC含量模板。pfu DNA聚合酶比Taq酶有更高的保真复制性能和热稳定性，更有利于高GC含量模板的扩增。

First,the content of surfactant should be more than 18 percent and the content of anion surfactant should be kept in proper proportion to the content of nonionic surfactant. Second,the content of zinc naphthenate should be kept in directly proportional to the content of other surfactant. Third,the content of rosin shouldnt too higher and the content of oleic acid should be kept in directly proportional to the content of rosin. Fourth,the content of water should be kept in a low level (less than 8%);otherwise emulsifier oil will delaminate. In addition,the requisite content of sodium heavy alkyl benzene sulfonate is higher than petroleum sulfonate.

试验表明在以松香、重烷磺酸钠、石油磺酸钠为主要乳化剂时，要获得优良的乳化液，需满足以下几个条件：(1)需在乳化油配方中加入适量的乳化剂，且其中阴离子乳化剂总量与非离子乳化剂总量的应保持适当的比例；(2)乳化油中环烷酸锌的量与其它乳化剂总量要保持正比关系；(3)乳化油中松香的量不易过大，且油酸的加入量应与松香量成正比；(4)乳化油中水分含量不易过高(一般不能大于8％)，过高时稳定性不好，易分层。

When that loss is large, float becomes expensive, sometimes devastatingly so.

损失，当损失过大时，就代表浮存金的成本过高，有时甚至高得离谱。

Through temperature、paracresol sodium salt concentration and the amount of BTC such as the study.

结果表明：酚钠盐浓度、温度以及BTC用量过高或过低，都会降低收率，其中温度较高会加速BTC的分解，而BTC用量较低会使反应不完全。

Secondly, within the expenditure for the development of education, hardware investment overweighs, while human resource investment is below a reasonable level.

同时，财政教育资源的配置结构不合理，在不同层次教育之间存在不合理因素，基础教育投入比例过低，高等教育投入比例过高；预算内教育经费使用构成不合理，人员经费比例过高；地区支出结构不合理等，影响了我国教育事业的发展，也不利于中国经济实现长期增长和均衡发展。

3Through analyzing the relation of providing and demand on housing market we can see that the imbalances of supplying structure and supply in district, mass backout and a great deal of gamble in house are important reason of high housing price.

3运用经济学中供求关系原理对南京市商品住宅市场供需状况分析可知，造成南京市商品住宅价格较高，涨幅较快的主要市场因素有：结构性供需不平衡、区域性供需不平衡，以及城市拆迁速度过快和投资性购房比例过高引起的对住房需求增长过快。

In a pilot scale reverse flow reactor with a liter of catalyst, catalytic combustion of typical industrial waste gas contaminated with volatile aromatic compounds was systematically investigated, and the "temperature run-away"in the case of too high feed concentration and the " extinction of reactor" in the case of low feed concentration were observed.

在流向变换催化燃烧小型中试装置上利用国产催化剂进行了含混合芳烃模拟工业废气的净化试验，重点考察了挥发性有机物能够通过调整换向周期在既不&飞温&也不&熄火&的条件下保持很高的VOCs转化率的浓度变化范围，初步观察了由于VOCs浓度过高或过低所引起的&飞温&或&熄火&过

The dissertation presents somecontents about the vacuum precooling as follows: 1. Studies on the evaporation cooling principles of fruit and vegetable There are different optimal storing conditions for different vegetables and fruits.And the vegetable and fruit precooled by vacuum must be fit with the storingcondition. A low temperature will result in the frozen scald, and a high temperaturewill not meet the precooling objective.

本文开展了以下几个主要方面内容的研究：1、果蔬蒸发冷却规律的研究不同果蔬都有各自不同的最佳储藏条件，因此，真空冷却后的果蔬必须满足储藏要求，温度过低易引起果蔬冻伤，过高则达不到预冷的目的；湿度过低易引起干耗，减轻重量；过高则易引起腐烂变质。

In order to produce lightweight cashmere and wool products of fashion with low cost and high added-value,a appropriate mixing ratio of water-soluble PVA fiber,cashmere and high-counts wool is selected,oil pick-up and moisture of blended top wool are strictly controlled,yarn twist and weaving tightness are carefully designed,and the vinylon dissolution process is optimized.

结果表明：水溶性PVA纤维所占混纺比例越高，可纺纱线密度就越低，但其所占比例过高则会造成坯布紧度及织造难度增大，退维后织物中纱线条干均匀度差，织物结构松烂；水溶性PVA纤维所占比例过小，则不易混合均匀，可纺纱线密度降低得少，达不到轻薄产品的要求。

The altostratus base is 2 km high, and cloud top is higher than 5 km. The supercooled water exists abundantly in the cloud, the maximum is up to 0.21 g.m-3 at -11.20℃, but its distribution is not homogeneous, 87%of continuous distribution width are less than 2.4 km, which is disadvantage to the rime increasing of ice crystal in the cloud. Meanwhile, the evaporation effect of subcloud raindrop is very big, the diameter of raindrops which could not arrive the ground is less than 1 mm. Therefore, The cold vortex process produces the weak precipitation.

As云底高2 km，云顶高大于5 km，云中存在比较大的过冷水区，-11.2℃时的过冷水含量0.21 g.m-3，但过冷水的含量分布极不均匀，大于0.05 g.m-3的过冷水区连续分布的宽度87%小于2.4 km，不利于云中冰晶的连续凇附增长；同时云下雨滴的蒸发效应大，直径小于1.0 mm的雨滴难以落到地面，因此本次过程只产生弱的降水。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！