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过滤叶片

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Glycolate oxidase (EC 1. 1. 3. 1, GO) was purified by 137. 4 fold from leaves of Brassica campestris L. ssp. chinensis var. utilis and the specific activity attained 130.9 U/mg. protein. Chromatography of purified GO through Sephadex G-200 showed two symmetric peaks of both protein and activity, which overlapped fully with each other. Further with electrophoresis on agrose plate, a protein spot and an activity spot were detected in electrophoretogram after respective staining, and the spot of protein covered that of activity, which indicated that the purified enzyme was of a high quality.

从菜心叶片中纯化了乙醇酸氧化酶,比活性达130.9 U/mg,纯化倍数为137.4倍;纯化酶经Sephadex G-200凝胶过滤显现单一而对称的蛋白吸收峰和活性峰,且两者完全重叠;进一步经pH 8.3缓冲系统的琼脂糖平板电泳、染色,各在负极端出现一个活性斑点和蛋白染色斑点,且两者的位置完全对应;表明纯化的菜心叶片乙醇酸氧化酶已达到较高的纯度。

Purified GO had a molecular weight between 145, 000 and 162, 300, as determined by Sephadex G-200 filtration and SDS-PAGE of the enzyme as cross-linked with glutaraldehyde. This result showed that the holoenzyme was a tetramer, and no change occurred in molecular weight of the enzyme during the s〓orage. The active form of GO from brassica leaves showed a constant molecular weight during purification, so did that from O. sativa leaves during greening of etiolated seedlings, maybe acting as a stable tetramer.

用Sephadex G-200胶过滤和戊二醛交联后的SDS-PAGE法测得纯化的菜心叶片GO的分子量在145,000-162,300间,为四聚体;并且在放置过程中其分子量和聚合态均保持不变,为稳定的四聚体;进一步研究表明菜心叶片GO在纯化过程中和水稻叶片GO在黄化苗的转绿过程中,两种GO活性状态的分子量和聚合态也没有发生变化,均呈四聚体。

The main products has: 200A-3 Oil Press, 202 type, 24 type, 240 type, 204-type pre-pressing machine, 1.5m 5 - 3.0m 5 steaming pan, various models of three-roll on-roll solution Pressure pressure embryo machine, Extrusion machine, drying machine, vibration fluidized bed dryer, frame-style crude oil filter, oil filter leaves and winter of filtering machines, centrifuges, sweetening pot, detreated pot, refining cans, from soap machine, than with mixers, heating cans, dehydrated machine, 28-36 decorticating machine, round screen, vibrating screen, flat screen, crusher, to stone machine, steam offline, deceleration machine, tube condenser, condensing plate , and plate heat exchangers, spiral plate heat exchanger, various scraper, cutter Liuzhou, hoist, 15-300 tons leaching equipment, 20-100 tons of salad oil equipment, one tons -20 tons of steam boilers, large 100000 -200 kcal million cards conducting oil furnace.

主要销售产品有: 200A-3 榨油机、 202 型、 24 型、 240 型、 204 型预榨机、 1.5m×5--3.0m×5 蒸炒锅、各种型号三辊、对辊液压压胚机、膨化机,烘干机、振动硫化床干燥机、板框式毛油过滤机、叶片滤油机,冬化过滤机,离心机、脱臭锅、脱色锅、精炼罐、脱皂机、比配混合机、加热罐、脱水机、 28-36 剥壳机、圆筛、振动筛、平面筛、破碎机、去石机、蒸脱机、减速机、列管冷凝器、板式冷凝器、板式换热器、螺旋板换热器、各种刮板,绞龙、提升机、 15-300 吨浸出设备, 20-100 吨色拉油设备, 1 吨-20 吨蒸汽锅炉, 10 万大卡-200 万大卡导热油炉。

The application of filtration equipment Products: plate and frame filter, candle filter, vertical leaf filter, horizontal leaf filter, etc.

该产品适用的过滤设备:板框过滤机、烛式过滤机、垂直叶片过滤机、水平叶片过滤机等

There were a great deal of kinds of the grass with high quality and quantity fed to fish To determine the best symbiosis ecosystem of fish and grass on a closed system circulated yearly, several cold and warm grass was selected to analyse the adaptability and nutrition absorbed. It indicted that Lolium multiform Lam. and lemarthria compressa were the best option due to high adaptability of wetness, high coarse protein, fat and fibre and so on in the leaf, high biomass, high bio-filtration effect of N and P, adaption to the low or high temperature, resist to the plant diseases and insect pests, and the food of fish whose forage was grass.

鱼用优质高产牧草的种类繁多,为确定最佳的鱼草共生周年循环系统,本文选择几种冷季牧草和暖季牧草进行生长适应性与营养吸收研究,分析表明,多花黑麦草和扁穗牛鞭草是最佳的冷季牧草和暖季牧草,它们耐水性强,叶片中所含的粗蛋白、粗脂肪、粗纤维等化学成分高,植株的生物量也高,生物过滤养殖废水中的氮和磷效果好,而且可以适应外界环境的低温或高温,抵抗病虫侵害,同时为草食性鱼类喜欢食用。

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