过氧化氢酶

It has been detected in a wide range of aerobe. The decomposition of hydrogen peroxide catalyzed by catalase is a bi- substrate enzyme-catalyzed reaction. As Chance proposed, it is an overall first-order reaction.

过氧化氢酶催化过氧化氢分解是一个两底物酶促反应，依照Chance提出的机理，反应速率方程具有一级反应方程的形式。

The properties of recombinant catalase and commercial catalase were compared.

将重组过氧化氢酶和市售一种商品过氧化氢酶的性质进行了比较。

Mmol/L. The activity of this catalase was not reduced by dithionite but was strongly inhibited by cyanide, azide, and 3-amino-1,2,4-triazole (the specific inhibitor of monofunctional catalase).

该过氧化氢酶不受连二亚硫酸钠的还原作用影响，但被氰化物、叠氮化物和3-氨基-1，2，4-三唑(单功能过氧化氢酶的专一抑制剂)强烈抑制。

In April 2004, 30 Boleophthalmus pectinirostris samples were collected from the lagune in Xiepu Villege, Zhenhai Town, Ningbo City, Zhejiang Province. Polyarylamide gel electrophoresis was used to detect the expression of isozymes in liver tissue. LDH, FDH, GDH, GCDH, G6PDH, MDH, ME, SOD, CAT and EST were analyzed and the biochemical genetic results showed that, among the 10 isozymes in this paper, 9 of which obtained clear enzyme patterns except for CAT.

于2004年4月，在浙江省宁波市镇海区澥浦滩涂采集野生大弹涂鱼30尾，采用聚丙烯酰胺凝胶电泳方法，分析了乳酸脱氢酶、甲酸脱氢酶、谷氨酸脱氢酶、D-葡糖脱氢酶、葡萄糖－6-磷酸脱氢酶(G6PDH)、苹果酸脱氢酶、苹果酸酶、超氧化物歧化酶、过氧化氢酶和酯酶等10种酶在大弹涂鱼肝脏组织中的表达，并对同工酶表型进行了生化遗传分析。

Unicolor during the period of florescence with the random block design of three factors. The results showed that the accumulation of MDA decreased. The content of soluble sugar, CAT activity and free proline increased with the increasing of potassium fertilizer application within 0 to 150 kg/hm^2. Potassium fertilizer application was more than 150 kg/hm^2, the result was opposite.

结果表明，施钾量在0~150 kg/hm^2范围内，随着钾肥施入量的增加，丙二醛含量降低，可溶性糖含量、过氧化氢酶活性和脯氨酸含量增加，但施钾量超过150 kg/hm^2时，丙二醛含量略有上升，可溶性糖含量、过氧化氢酶活性和脯氨酸含量有所下降。

The six stages involved euthermic state (25℃), prehibernation (10-12℃), after hibernating for a week and a month (2-4℃), and arousing from hibernation for a week and two weeks (10-12℃). From euthermic state to long-term hibernation, lipid peroxidation was showed to increase in brain except the stage of prehibernation.

分别测试了上述各阶段荒漠沙蜥1％脑组织匀浆、0.5％心脏组织匀浆及1％肝脏组织匀浆中：丙二醛及过氧化氢(H_2O_2)的含量，超氧化物岐化酶、过氧化氢酶、谷胱甘肽过氧化物酶的活性，及总抗氧化能力的水平。

Pentaphyllum s influence on the activity of Glutathione peroxidase and Catalase in the skin of hireless photoageing mice as well as the content of hydrogen peroxide in their skin, and to investigate G.

目的：研究外用绞股蓝对紫外线照射引起的无毛小鼠皮肤光老化模型皮肤组织谷胱甘肽过氧化物酶、过氧化氢酶的活性、过氧化氢(H_2O_2)含量，以及无毛小鼠真皮结构变化的影响，判断外用绞股蓝对皮肤光老化的作用。

Pentaphyllum s influence on the activity of Glutathione peroxidase and Catalase in the skin of hireless photoageing mice as well as the content of hydrogen peroxide (H_2O_2) in their skin, and to investigate G.

目的：研究绞股蓝提取液对紫外线照射引起的无毛小鼠皮肤光老化模型皮肤组织谷胱甘肽过氧化物酶、过氧化氢酶的活性、过氧化氢(H_2O_2)含量，以及无毛小鼠真皮结构变化的影响，判断内服绞股蓝提取液对皮肤光老化的作用。

Using the KMnO4 titration method to detect the catalase activity and the sulfosalicylic acid method to detect the free proline content, the changes of catalase activity and free proline content of plants in Kaili area under air pollution were studied with the representative plants such as Camellia oleifera, Pinus massonina, Cunninghamia lanceolata, Woodwardia japonica and Dicranopteris pedata in Kaili area as research subjects.

方法］以凯里地区的代表性植物（油茶、马尾松、杉树、狗脊蕨和铁芒萁）作为研究对象，采用KMnO4滴定法测定过氧化氢酶的活性，采用磺基水杨酸法测定游离脯氨酸的含量，研究大气污染影响下凯里地区植物过氧化氢酶活性、游离脯氨酸含量的变化情况。

Nested PCR analysis and southern hybridization analysis showed that no polymorphism between H.villosa, Pm97034 and susceptible parent Wan7 107 was detected with the clones from 6VS arm, whereas three clones from H.villosa genome DNA: RH42, RH55, and RH66 showed polymorphism. RGA6 cloned from H.villosa genome DNA was characterized identity to NBS, and show high homology to resistance genes as RPM1 and RPP13 in Arabiadopsis, LRR19 in wheat, and I2C-1 in tomato.cDNA library constructed from T.aestivum-H.villosa translocation line Pm97034 was screened by hybridization with RGA6. Four positive cDNA clones were obtained. R3-2-2 showing 60-90% identity with eukaryotic sulfite oxidases contained an open reading frame of 647bp encoding 140 amino acid, and contained a conserved Moco-dimer domain in the ORF. R6-2-2 showed an ORF containing an Euk-porin domain of 279aa with 20-40% identity to the eukaryotic voltage-dependent anion channel proteins. R8-1-1 showed a complete ORF of 1810bp encoding 493aa with 80-90% identity to plant catalases. R9-1-1 showed an ORF containing a BTB/POZ conserved domain of 204aa with 30-70% identity to pox virus and zinc finger proteins. R3-2-2 and R9-1-1 were the first cDNA clones containingconserved domain of SO and POZ respectively isolated from wheat. R8-1-1 contained a complete ORF with 81% identity to Cat-3. Aspects of the role of R8-1-1 may be same with Cat-3, and it would offer the opportunity for improvement of stress tolerance of wheat.

以RGA6为探针，筛选用抗白粉病小麦—簇毛麦易位系Pm97034构建的cDNA文库，得到了4个阳性克隆。R3-2-2与动植物的亚硫酸氧化酶(sulfite oxidase，SO)有60～90％的同源性，长度为647bp，编码140个氨基酸，具有开放阅读框并含有保守域Moco-dimer.R6-2-2与真核生物的VDAC(voltage-dependent anion chennel)蛋白有20～40％的同源性，长度为1047bp，编码279aa，是一个不完整的开放阅读框，预测结构具有Euk-porin结构域。R8-1-1与植物中已经克隆的过氧化氢酶有80～90％的同源性，长度为1810bp，编码493aa，具有完整的开放阅读框和过氧化氢氧化酶保守域。R9-1-1与动植物的POZ(pox virus and zinc finger protein)蛋白有30～70％的同源性，长度为1446bp，具有开放阅读框和BTB／POZ保守域。R3-2-2与R9-1-1是首次从小麦中克隆到的具有SO和POZ保守域的cDNA序列。R8-1-1具有完整的开放阅读框，与玉米Cat-3基因具有81％同源性，预测R8-1-1可能具有与Cat-3类似的功能，可为转基因小麦抗逆育种提供新的基因。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。