过染

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

A usual occasion to find this trick in use is in press conferences, where spokespersons turn to giving nonsense answers to sharp, embarrassing questions instead of using "no comment" to divert reporters' attention, while trying to give the impression of being humorous, witty and charmful at the same time.

六、甚至还有一种预感， Mary 并未见过这位继母， Mary 说她是个 blonde，很可能是引述她父亲的原话。这个帖子发表之后不久，又有网友根据 blonde 这个单词的本意，又补充了两个隐含意思：(1)她以前染过金发，(2)她现在又把金发染成了另外一种颜色。

Objective: To colone SOD gene, construct combinant of retrovirus SOD gene, make a basis of gene transfection and over-expression of gene therapy.

目的 通过克隆SOD基因，构建逆转录病毒-SOD真核表达载体转染复合体，为基因转染及SOD的过表达提供基础，为将来的基因治疗进行准备，也为深入研究SOD功能及SOD的开发创造条件。

Results are as followed:1 Exposure of HELF cells to BP caused c-Jun activation,and increased the activity of MAPK,PI-3K,p53 and cyclin D1 pathway.2 BP-induced c-Jun activation was inhibited by dominant negative mutants of extracellular signal-regulated protein kinase or c-Jun NH_2-terminal kinase,but not by p38,impling that JNK and ERK pathways medicate c-Jun activation induced by BP.3 Overexpression of dominant-negative mutants PI-3K and Akt potently blocked phosphorylations of c-Jun and ERK,but not JNK in response to BP,suggesting that PI-3K/Akt pathway positively regulates BP-induced c-Jun activation through ERK.4 Inhibition of p53 by its chemical or molecular inhibitor markedly increased the phosphorylation levels of c-Jun,Akt and ERK upon BP stimulation,indicating that p53 negatively medicates BP-induced c-Jun activation through PI-3K/Akt/ERK pathway.5 The cell lines expressed TAM67 exhibits no significant affecting normal cell growth properties.6 TAM67 was able to significantly block G_1-S transition and subsequent cell proliferation,suggesting that c-Jun is essential for cell cycle alternations elicited by BP.7 Overexpression of TAM67 impaired BP-induced cyclin D1 activation,decreasing expression of E2F1 and pRb,indicating that c-Jun participates in the modulation of BP-induced activation of cyclin D1/pRb/E2F1 pathway.8 Stably expression of TAM67 led to the increases in the expression levels of p53 and p21,elevating phosphorylation level of p53,clearly indicating that c-Jun regulates p53/p21 pathway activation induced by BRCollectively,PI3K/Akt/ERK pathway mediated BP-induced c-Jun activation through p53-dependent mechanism.

结果显示：1BP刺激细胞可促进c-Jun活化，并伴随着MAPK、PI-3K、p53和cyclinD1通路各组成成分的活性增强。2利用MAPK通路的显性失活突变体分别阻断细胞外信号调节激酶和c-Jun氨基末端激酶活性，均可明显抑制BP诱导的c-Jun活化，但阻断p38活性对BP引起的c-Jun活化无明显影响，提示JNK和ERK通路参与调控BP诱导的c-Jun活化。3过表达PI-3K和Akt的显性失活突变体也可显著抑制BP诱导的c-Jun活化，并降低磷酸化ERK的表达水平，但对磷酸化JNK的表达水平无明显影响，说明PI-3K/Akt通路通过ERK正性调控了BP诱导的c-Jun活化。4p53的化学/分子抑制剂能使BP作用的细胞内c-Jun活性明显增加，并同时诱导Akt和ERK的磷酸化水平的升高，表明p53可通过PI-3K/Akt/ERK通路对BP诱导的c-Jun活化进行负性调控。5随后观察转染细胞的生长情况，发现TAM67对细胞正常生长和形态无明显影响。6稳定表达TAM67可有效抑制BP诱导的S期细胞数的增加，提示c-Jun在BP致细胞周期改变的过程中发挥了重要作用。7TAM67过表达能够抑制BP诱导的cyclin D1活化，降低磷酸化Rb以及E2F1蛋白表达水平，表明c-Jun参与调控BP诱导的cyclin D1/Rb/E2F1通路的活化。8过表达TAM67可使BP刺激的细胞中p53、p21总蛋白以及p53磷酸化的表达水平明显升高，可见c-Jun也参与调控BP诱导的p53/p21通路活化。

There is a long standing debate about the magma origin of Lutao. Two different models of crust contamination and source contamination are proposed. Combining helium results with strontium ratios of Lutao lavas, it shows that Lutao arc magma has experienced approximately 5% of crust contamination.

绿岛岩浆成因长久以来存在有地壳混染或是源区混染的争论，本研究利用绿岛斜辉石及堇青石巨晶的3He/4He比值及其母岩浆的87Sr/86Sr比值，得到了绿岛岩浆经历过5%左右地壳混染的结果。

The optimum dyeing conditions for the WP/PP fiber was as the following: the dye temperature was 95℃, the dyeing time was 45min, the concentration of carrier Dilatin POE was 0.8g/L. The color fastness to laundering, perspiration and crocking were excellent after the WP/PP fiber has been dyed with disperse dyes of Foron.

结果表明：与纯PP纤维相比，WP/PP纤维的染色性能有了很大程度的改善，上染率提高1倍以上；优化染色工艺为：染色温度95℃，染色时间45min，载体Dilatin POE的质量浓度0.8g/L； Foron类分散染料染过的WP/PP纤维具有良好皂洗牢度、汗渍牢度和摩擦牢度。

I began now to think my cave or vault finished, whenon a sudden (it seems I had made it too large) a great quantity ofearth fell down from the top on one side; so much that, in short,it frighted me, and not without reason, too, for if I had beenunder it, I had never wanted a gravedigger.

虽然小时候父亲一直谆谆教诲我，但八年来，我一直过着水手的生活，染上了水手的种种恶习；我交往的人也都和我一样，邪恶缺德，不信上帝。所以，我从父亲那儿受到的一点点良好的教育，也早就消磨殆尽了。这么多年来，我不记得自己曾经敬仰过上帝，也没有反省过自己的行为。

Results:AFI method was much better than AFV in diagnosis of oligohydramnios by ultrasonography.The combination of both methods could obviously improve the diagnostic accuracy of oligohydramnios.Oligohydramnios could cause pregnant complications,the rate of cesarean section,fetal distress,asphyxia neonatorum and meconium -stained fluid improved significantly.

结果：超声诊断羊水过少AFI法优于AFV法，两种方法结合可明显提高羊水过少的诊断准确率，羊水过少易伴发妊娠并发症，且妊娠晚期羊水过少孕妇剖宫产率及胎儿窘迫、羊水粪染、新生儿窒息的发生率明显增加，差异均有显著性。

objective:to explore the method of ultrasonography in the diagnosis of oligohydramnios in late pregnancy,the association of olignophdramnios with pregnant complications and its effect on perineonate.methods:the clinical data in 84 patient with oligohydramnios from january 2004 to december 2006 in our hospital were collected,and 100 patients with normal amniotic fluid volume selected randomly served as control at the same time.the complications of pregnancy and adverse perinatal outcomes were analysed.results:afi method was much better than afv in diagnosis of oligohydramnios by ultrasonography.the combination of both methods could obviously improve the diagnostic accuracy of oligohydramnios.oligohydramnios could cause pregnant complications,the rate of cesarean section,fetal distress,asphyxia neonatorum and meconium -stained fluid improved significantly.conclusion:oligohydramnios in late pregnancy can cause adverse effect to perineonate,it is a dangerous omen to perineonate and need to be done immediately.ultrasonography is a dependable diagnostic method of oligohydramnios in late pregnancy.

目的：探讨超声诊断晚期妊娠羊水过少的方法，羊水过少与妊娠并发症的关系及对围生儿的影响。方法：收集我院2004年1月~2006年12月住院分娩足月妊娠产妇超声诊断羊水过少者84例，与临床符合率相比较，同时随机抽取同期在我院住院分娩的羊水量正常孕妇100例作为对照组，两组妊娠并发症及围生儿情况进行比较。结果：超声诊断羊水过少afi法优于afv法，两种方法结合可明显提高羊水过少的诊断准确率，羊水过少易伴发妊娠并发症，且妊娠晚期羊水过少孕妇剖宫产率及胎儿窘迫、羊水粪染、新生儿窒息的发生率明显增加，差异均有显著性。结论：妊娠晚期羊水过少会给围生儿造成不良的影响，是危险的征兆，需及时处理，超声检查是产前诊断羊水过少的可靠方法。

As the wax film in printing will be bent from time to time arising from the irregular small cracks, stain penetration too small cracks in the cotton dyeing a chic lines, becoming the unique ambience hand batik printed cloth, the traditional ethnic batik printed cloth with indigo dye, printed blue white flower pattern, graphics, mostly birds and flowers and so on, due to differences in habits of various nationalities has its own style.

由于蜡膜在印染时常会被弯折而出现不规则的细小裂纹，染液渗过小裂纹而在棉布上染出别致的纹路，成为手工蜡染花布的特有情调，传统的民族蜡染花布以蓝靛为染料，印出蓝底白花图样，图形多数为花鸟等，因各民族习惯有差别而具有各自的风格。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷