输卵管的

METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要：通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt；采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中，然后将注射存活的受精卵植入假孕母鼠的输卵管；取其产3 wk龄子代鼠进行PCR初选，再通过Southern杂交进一步确证；利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

With the fast development of biopharmacy, transgenic chicken oviduct bioreactor is going to be the focus of people's attention. For its excellent advantages, transgenic chicken will be one of rising industries in the field of biopharmacy and hot spot of scientific study.

随着生物制药业的迅速发展，转基因鸡输卵管生物反应器正逐渐成为人们关注的焦点，转基因鸡以其卓越的优势必将成为科学研究的热点和生物制药领域的新兴产业之一。

Lamin A gene cDNA lacking of 150 basyls at the end of the eleventh extron was cloned into pcDNA3.1 carrier, and the recombinant plasmid pcDNA3.1-mutant LMNA cDNA was injected into the male pronucleus of mouse zygotes, then the zytotes was replanted into the oviducts of pseudopregnant mice.

实验方法：将缺失了第11外显子末端150个碱基的LMNA基因cDNA克隆到pcDNA3.1载体，应用原核显微注射方法将线性化的pcDNA3.1-mutant LMNA注射到小鼠受精卵的雄原核，再将注射后的受精卵移植至假孕鼠的输卵管。

Immunohistochemical method was used to detect avian leukosis virus subgroup J from egg-type chicken, which was preliminarily diagnosed to appear subgroup J avian leukosis. The organs from these chickens were examined by ALV-J gp85 monoclonal antibody, these organs included marrow, liver, spleen, kidney, lung, heart, pancreas, oviduct, ovary, ventriculus glanduaris, skeletal muscle, cerebrum, ischiacticus nerve.

采用免疫组化法，对病理学初步诊断为蛋用型鸡 J亚群白血病的自然发病鸡的肿瘤、骨髓、肝脏、脾脏、肾脏、肺脏、心脏、胰脏、输卵管、卵巢、腺胃、骨骼肌、大脑、坐骨神经，用特异性抗 J亚群禽白血病病毒囊膜糖蛋白gp85的单克隆抗体进行检测，待检的组织切片中均检出阳性抗原，免疫组化的研究结果与病理学诊断结果相一致。

Some archaeologists have theorized that the ancient belief in the sacredness of the horned animal head -- specifially the bull's head or bucranium -- derives from its coincidental resemblance to the female human genitals, consisting of a vagina, uterus, fallopian tubes, and ovaries.

一些考古学家推理，古老信仰在有角的动物头的神圣-- specifially 公牛的头或bucranium --从它的巧合相似获得对女性人的阴部，包括阴道、子宫体、输卵管，和卵巢。

Figure 3. A, Transabdominal scan of the right adnexa shows a complex mass surrounded by pelvic structures containing several round areas of loculated fluid that vary in size and are separated by a thickened band or adhesion, simulating a tubo-ovarian abscess or complex. B, Transabdominal cross-sectional scan shows the 2 loculated fluid areas posterior to the uterus separated by a thickened adhesion band.

图3 A，右侧附件区经腹部扫描显示被盆腔结构环绕的混合性肿块，其内有数个圆形的局限性积液，其大小不等，被增厚的束带或粘连分隔，象是一个输卵管-卵巢脓肿或复合体。B，经腹部横切面扫描显示子宫后方的2个局限性积液区，被一增厚的粘连束带所分隔。

Figure 3. A, Transabdominal scan of the right adnexa shows a complex mass surrounded by pelic structures containing seeral round areas of loculated fluid that ary in size and are separated by a thickened band or adhesion, simulating a tubo-oarian abscess or complex. B, Transabdominal cross-sectional scan shows the 2 loculated fluid areas posterior to the uterus separated by a thickened adhesion band.

图3 A，右侧附件区经腹部扫描显示被盆腔结构环绕的混合性肿块，其内有数个圆形的局限性积液，其大小不等，被增厚的束带或粘连分隔，象是一个输卵管-卵巢脓肿或复合体。B，经腹部横切面扫描显示子宫后方的2个局限性积液区，被一增厚的粘连束带所分隔。

PurposeThe present study was designed to pursue the kinetic characteristics of acrosome reaction of sperm in the fallopian tube in vivo, to evaluate the effect of human oviductal epithelial cell on the AR capacity of sperm and the possible mechanisms through the application of coculture system with HOEC in vitro.

目的 通过检测与人输卵管上皮细胞共培养的人精子在不同时间的顶体反应率，探讨精子在输卵管内AR过程的特点，分析HOEC对精子AR的影响和可能机制。

The rate of morulae/blastocysts was (59.5±3.2)%(P.05). In conclusion, the sequence culture system (SOF and granulosa cell+NCSU-23 FCS and POEC) can improve the development of procine parthenogenetic embryos.

结果表明，SOF培养基和颗粒细胞＋添加胎牛血清的NCSU-23和输卵管上皮细胞的序贯培养系统能较好的促进胚胎的发育。

Construction of heavy chain transgenic mice and genome type analysis Linearized transgene plasmids were microinjected into pronuclei of zygotes from CBA×C57BL/6 mice, and transplamted into oviduct of pseudo-pregnant mice.

二、抗体重链转基因小鼠建立及基因型分析将线性化的质粒显微注射入CBA×C57BL/6小鼠的受精卵细胞的雄前核内，然后导入假孕雌鼠的输卵管。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。