辅酶

The acetyl-CoA carboxylase is the rate-limiting enzyme of fatty acids synthesis.

乙酰辅酶A羧化酶催化脂肪酸合成的第一步，是脂肪酸合成的限速酶。

In addition, SW480 and HCT116 cells were treated with deoxycholic acid for 15, 30, 45, 60, 90 and 120 minutes. It was found that the deoxycholic acid treated cells showed an elevation of ATP activity by 12.35 %, 20.08 %, 29.69 %, 33.56 %, 45.85 %, 54.32 %, respectively. While the HCT116 treated cells, showed an elevation of ATP activity by 3.69 %, 5.59 %, 8.52 %, 12.26 %, 16.52 %, 19.71%, respectively.From the experiments conducted using the cell lines described above, it was further confirmed that genes CYP7A1 (cytochrome P450, family 7, subfamily A, polypeptide 1), AKR1D1 (aldo-keto reductase family 1, member D1), ALDH1A1 (aldehyde dehydrogenase 1 family, member A1), BAAT (bile acid Coenzyme A: amino acid N-acyltransferase), ABCA1 (ATP-binding cassette, sub-family A, member 1), APOC3 (apolipoprotein C-III), LIPA, LIPE (lipase, hormone-sensitive), PTGER2 (prostaglandin E receptor 2) and PTGS2 (prostaglandin-endoperoxide synthase 2) were up-regulated.

此外，为了证实其脂肪代谢产物胆酸对细胞癌化的影响，亦使用SW480和HCT116细胞株，经由脱氧胆酸在15分钟、30分钟、45分钟、60分钟、90分钟和120分钟处理后，其细胞增生比率分别有12.35%，20.08%，29.69%，33.56%，45.85%和54.32%(p.05)以及3.69%，5.59%，8.52%，12.26%，16.52%和19.71%(p.05)上升情形，并从这些细胞株进一步证实CYP7A1(细胞色素P450-7A1)、AKR1D1(醇醛酮还原酶-1-D1)、ALDH1A1(醛脱氢酶-1-A1)、BAAT(胆酸辅酶A：氨基酸N-醯基转移酶)、ABCA1(三磷酸腺苷结合盒转运体A1)、APOC3、LIPA、LIPE(脂肪酶/激素敏感脂肪酶)、PTGER2(前列腺素E受体2)和PTGS2(前列腺素内过氧化物合成酶2)等基因表现皆有呈现向上调控的情形。

The results imply that the inhibition of NADPH is due to the earlier binding of NADPH to the enzyme which caused the arm to move from the condensation domain to the reduction domain so as to affect the binding of substrate malonyl CoA and inhibit the activity.

实验结果表明：NADPH对脂肪酸合成酶的抑制可能是由于NADPH在酶上过早的结合而使该酶的活臂向远离缩合中心的方向移动，从而影响了底物丙二酰辅酶A与酶的结合，造成酶活性的下降。

The oversaturation of cholesterol in bile results in the formation of cholesterol stones. The liver is the organ which synthesizes cholesterol and secretes bile. Liver lipid metabolism research helps to explain the mechanism of cholelithiasis. Utilizing biochemical and biomolecular technology, we have done experimental work as follows: Analyse the ingredient changes of blood and bile samples from patients.

肝脏是合成胆固醇、分泌胆汁酸的器官，对肝脏胆固醇代谢的研究有助于阐明胆石症形成的原因，本文用生物化学和分子生物学的方法，分析了病人血液、胆汁中各种脂类成份的变化，以及肝脏胆固醇合成限速酶3-羟-3-甲戊二酸辅酶A还原酶（HMG-CoA Reductase）、胆汁酸合成限速酶7α-羟化酶（7α-hydrolase）的基因表达。

Pyridoxine, pyridoxal , pyridoxamine as well as their phosphated forms are structural analogues in which pyridoxal-5"-phosphate and pyridoxamine-5"- phosphate are the biological active forms in vivo which act as the cofactors of aminotransferases, amino acid decarboxylases, cysteine desufbrase and cystathionine , etc by constant transconversions. Recently, it was discovered that B6 played a novel role in tumor growth suppression.

它包括三种结构十分近似的化合物：吡哆醇(Pyidoxine，PN)、吡哆醛(Pyridoxal，PL)和吡哆胺(Pyridoxamine，PM)，分别以磷酸化的形式发挥作用，其中吡哆醛-5'-磷酸(Pyridoxal-5'-phosphate，PLP)为B_6在体内的活性形式，通过与磷酸吡哆胺(Pyidoxamine-5-phosphate，PMP)的互变，作为多种酶(氨基转移酶、氨基酸脱羧酶、半胱氨酸脱硫酶、胱硫醚酶等)的辅酶而发挥传递基团的作用。

The effects of side-chain precursor-Solanesol, ubiquinone cicle precursors-phydroxybenzoic acid, and coenzyme Q_0 on biosynthesis of coenzyme Q_(10) were studied.

首次研究了以辅酶Q10 (CoQ10 )主要侧链供给前体物质———茄尼醇和醌环供给前体———羟基苯甲酸和辅酶Q0 对粟酒裂殖酵母(Schizosaccharomycespromb 2 1794 - 2 3)生产CoQ10 产量的影响，并对前体的转化工艺进行了初步研究。

Optimization of precursors and elicitors for taxol production by cell suspension culture of Taxus chinensis;2. The effects of side-chain precursor-Solanesol, ubiquinone cicle precursors-phydroxybenzoic acid, and coenzyme Q_0 on biosynthesis of coenzyme Q_(10) were studied.

首次研究了以辅酶Q10 (CoQ10 )主要侧链供给前体物质———茄尼醇和醌环供给前体———羟基苯甲酸和辅酶Q0 对粟酒裂殖酵母(Schizosaccharomycespromb 2 1794 - 2 3)生产CoQ10 产量的影响，并对前体的转化工艺进行了初步研究。

The five prosthetic groups (FAD, [2Fe-2S], [4Fe-4S], [3Fe-4S] and heme group) required for electron transfer from succinate to ubiquinone were unambiguously assigned into the electron density map. Besides, we find there are some electron densities around the Qp pocket at the matrix side and we believe it represents the head structure of ubiquinone, which is proved by the inhibitor bound structure that 2-TTFA just locating at the Qp site. At the same time, we find there is the second 2-TTFA binding site, locating the inter-membrane side. This finding will change our knowledge about the electron transfer inside Complex II and the ubiquinone transfer between Complex II and Complex III, and endow a new role of Complex II in electron transfer chain.

除了对各个电子传递体（ FAD ，[2Fe-2S]，[4Fe-4S]，[3Fe-4S]以及血红素分子）进行精确定位外，我们在该结构跨膜区靠近线粒体基质一端的口袋 Qp 中，发现了一些电子密度，认为是所结合的辅酶 Q 的头部结构，这一点被与抑制剂结合的复合体的结构所证明，在该结构中， 2－ TTFA 恰好结合在口袋 Qp 中，同时，我们还发现了第二个2－ TTFA 的结合位点，位于跨膜区靠近线粒体膜间隙一端的口袋 Qd 中，这个发现具有全新的意义，将影响人们对电子在复合物 II 中传递以及辅酶 Q 在复合物 II 与 III 之间转移的认识，促使人们重新复合物 II 在线粒体呼吸链中的角色。

The effects of side-chain precursor-Solanesol, ubiquinone cicle precursors-phydroxybenzoic acid, and coenzyme Q0 on biosynthesis of coenzyme Q10 were studied. The feasibility of the precursors biotransformation to CoQ10 by Schizosaccharomyces promb 2.1794-23 was also disscussed in the article.

首次研究了以辅酶Q10(CoQ10)主要侧链供给前体物质－茄尼醇和醌环供给前体－羟基苯甲酸和辅酶Q0对粟酒裂殖酵母(Schizosaccharomyces promb 2.1794-23)生产CoQ10产量的影响，并对前体的转化工艺进行了初步研究。

The effect of the flight on the activities of energy metabolism related enzymes in the flight muscles of the Oriental armyworm moth, Mythimna separata females was studied. These enzymes measured were glyceraldehydes-phosphate dehydrogenase, glycerol-3-phosphate dehydrogenase, lactate dehydrogenase, and 3-hydroxyacyl-CoA dehydrogenase. In the first 5 min of the tethered-flight of 3 day-old moth, activities of all the enzymes related to carbohydrate and lipid metabulistu increased rapidly, enzymes related to lipid metabolism were completely activated, and the activities of HOAD were significantly strengthened. But in flight duration from 5th to 60th min, the activities of all energy metabolism related enzymes decreased, suggesting that the flight was going to be stabilized.

对3日龄粘虫雌蛾吊飞过程中4种相关酶3-羟酰辅酶A脱氢酶、3-磷酸甘油醛脱氢酶、3-磷酸甘油脱氢酶和乳酸脱氢酶的研究结果表明，在室内条件下，粘虫在吊飞过程中其能量代谢有以下特点：在吊飞的初始5min，所有与糖代谢和脂肪代谢相关的酶活性都快速升高，这段时期脂肪代谢的酶活性也完全被活化，HOAD活性明显增强；但在随后的5~60min持续吊飞期间与能量代谢有关的酶活性都有所下降，表明此时飞行活性趋于平稳。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。