轴质

Howeer, few studies hae looked at changes of neurons within the caused by disturbance of axonal flow, the axon reaction, chromatolysis, and cell death as a result of mechanical compression of the entral

但是， 很少有研究关注腹侧神经根机械性压迫所致的神经元改变，如轴流，轴索反应，染色质溶解，细胞死亡等。

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

BACKGROUND: Myelination following axonal regeneration is a key factor affecting the recovery of spinal cord injury.

背景：轴突再生后髓鞘化是影响脊髓损伤后恢复的一个关键性因素，而少突胶质细胞存活的多少直接影响轴突再生后髓鞘化。

Results there were unconsciousness, respiratory rhythm disturbance and hyporeflexia of pupil light reflex immediately after injury, and reactiveness decrease and activity retardation still existed even after resuming consciousness. at anatomical scene, subarachnoid hemorrhage or cerebroventricular haemorrhage were widespread. at an early stage, there were swelling, collapse, and axonal retraction ball formation at cortico-medulla junction, callosum, brainstem, and cerebellar white matter under microscope. but at the later stage, gitter cell proliferation and nest-like aggregation were major pathophysiological changes at focal brain tissue.

结果 伤后大鼠均即刻出现原发昏迷，其中2只于损伤后20 min内死亡，余持续时间1-30 min不等；伤后大鼠呼吸节律紊乱，瞳孔对光反射减弱或消失，醒后均有程度不等的反应性下降，肢体活动迟缓；肉眼可见广泛蛛网膜下腔出血或脑室出血；光镜下可见皮髓交界区、胼胝体区、脑干、小脑白质等部位的神经轴索有不同程度的肿胀、断裂、轴索球形成，后期有小胶质细胞增生，局部呈巢样聚集。

D1.1 of the AISI Specification limits the maximum longitudinal spacing of connections to ... where smax = maximum permissible longitudinal spacing of connectors L= unbraced length of compression member rI = radius of gyration of I-section about the axis perpendicular to the direction in which buckling would occur for the given conditions of end support and intermediate bracing rcy = radius of gyration of one C-section about its centroidal axis parallel to the web.....

因此，AISI规范第D1.1节限定连接的最大纵向间隙为：，式中 smax =连接体允许的最大纵向间隙 L=抗压构件的自由长度 rI =工字钢对垂直于给定端承和中间肋条件下可能产生纵向弯曲方向的轴的回转半径 rcy =一段U型钢对其平行于筋的质心轴的回转半径。。。。。。。

During dehydration, the tolerance of wampee axes to desiccation which was analyzed as germination percentage, vigour index, and rate of electrolyte leakage, and activities of antioxidant enzymes gradually decreased, the content of malondialdehyde , progressively increased.

黄皮种子离体胚轴在脱水过程中，胚轴的萌发率、活力指数、电解质渗漏速率，超氧化物歧化酶、过氧化物酶和过氧化氢酶活性逐渐降低，膜质过氧化产物MDA的含量不断增加。

In SMC group, relative stable of the both ends of fracture reduce unnessary repeated stimulation of injury, and at same time, axio-stress advantageous for preosteoblast and mesenchymal cell transmit to osteoblast and chondroblast. Material and design superiority of SMC form unique internal mechanical environment, and which benefit for bone healing. The study took deep investigation of SMC on fracture healing.

上述结果证实：SMC内固定可对骨折端施加主动的、持续的轴向应力刺激，形成沿长骨骨干轴向的成骨导向力值，配合环抱翼的把持作用，形成了区别于CP、IN的独特的稳定、有效的机械内环境，骨折端的相对稳定减少了不必要的损伤再刺激，同时持续轴向应力有利于成骨祖细胞、间充质细胞向成骨细胞和成软骨细胞转化、促进了成骨诱导过程的发生与发展，加快了骨折愈合的修复与重建。

The main vibratory system of the screen consists of the balance substance and shear rubber spring while the vibration isolation system is composed of the balance substance and vibration isolation spring.

筛机由机体平衡质体与简切橡胶弹簧组成主振系统，机体平衡质体与隔振弹簧组成隔振系统，筛机的振动是由装于平衡质体中间的单轴惯性激振器激励的。

Measured the basic physical parameters of unsaturated soils in Three Gorges Reservoir area; 2 measured the initial sample matric suction using the unsaturated triaxial testing system, which can be used to evaluate the unsaturated soil strength and stress; 3 the drying and moisture absorption experiments were executed using the Tempe apparatus, and got the Soil-Water Characteristic Curve, permeability coefficients of every stress level were simulated according to the parameters from the SWCC; 4 the unsaturated soil strength tests were carried out using the GDS triaxial testing system, then got the Mohr circle and shear strength parameters; the results from different stress paths were compared; meanwhile, the axial strain-stress ratio-volume strain curve of different matric suction and different radial pressure was got, as well as their relationship; The research, which provided the necessary data and reference for Three Gorges Reservoir area geologic disaster, is the basic component of Three Gorges Reservoir geologic disaster prevention and research, and experimental results will be used directly in the project.

1量测了三峡库区非饱和土的基本物性参数； 2用非饱和土三轴试验仪量测了试样的初始基质吸力，为评估非饱和土的强度和应力水平提供参考； 3用Tempe仪和体积压力板仪进行了非饱和土的干燥和吸湿试验，得到了土-水特征曲线，并根据其参数拟合出各级吸力状态下对应的渗透系数； 4用GDS三轴仪进行了强度试验，得到了Mohr圆和抗剪强度参数；并比较分析了不同应力路径下试验结果；同时得到不同吸力和不同围压下轴向应变-应力比-体应变的关系曲线和其相互关系。该研究是三峡库区地质灾害防治研究工作的基础性工作，其结果将直接应用到工程实际中，为三峡库区地质灾害提供必要的数据和参考。

Human hair keratin ; spinal cord injury ; rat ; tissue engineering ; tissue-engineered spinal cord ; connector ; morphology ; praxiology ; neuron ; axis-cylinder ; nerve fiber ; myelin sheath ; astrocyte ; oligodendrocyte ; microglia ; ependymal cell ; neural stem cells ; macrophage ; multinuclear giant cell ; lysosomal enzyme ; ubiquitin system ; suppression subtractive hybridization ; gene express ; CytohromeoxidaseⅢ; amino acid transporter system A2（Ata2）; Peroxiredoxins 5（prd 5）; cysteine proteinase inhibitor cystatin c

国家自然科学基金；广东省自然科学基金人发角蛋白；脊髓损伤；大鼠；组织工程学；组织工程化脊髓；桥接物；形态学；行为学；神经元；轴突；神经纤维；髓鞘；星形胶质细胞；少突胶质细胞；小胶质细胞；室管膜细胞；神经干细胞；巨噬细胞；多核巨细胞；溶酶体酶；泛素肽酶系；抑制消减杂交；基因表达；细胞色素氧化酶Ⅲ；氨基酸转运蛋白系统A2；抗过氧化物蛋白5；半胱氨酸蛋白酶抑制剂

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。