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The neural network model simulates the visual characteristics of the human visual system by axons delay pulse neural network in order to extract the moving target and anlyse the actual significance of pulse neuroid algorithm.

这里提出一种基于神经轴突延迟机制,利用脉冲神经元网络模型对运动目标进行检测与跟踪的算法,该神经网络模型是利用轴突延迟脉冲神经网络模拟人类的视觉特性,从而提取运动目标,并具体分析脉冲神经元网络算法的实际意义。

Cells were collected to undergo HE staining and immunocytochemistry reaction against Thy-1 to identify RGCs. Axons length of retinal neurons and RGCs were measured by microruler under microscope respectively to evaluate the neurotropic effects of B vitamins in improving the regeneration and growth of axons for retinal neurons and RGCs. Finally, we observed the reaction of retinal neurons when exposed to the insult of glucose deprivation. The neuroprotection of B vitamins were also evaluated at the same time.

对B族维生素的神经营养和保护作用的研究过程分为三个阶段,首先确定维生素B族对视网膜神经元是否有神经营养作用,并根据细胞活力找出最佳有效作用浓度;然后在接种细胞的即刻用各种维生素的最佳有效作用浓度作用于视网膜神经元,并在不同时间终止培养,收集细胞,HE染色和RGCs的免疫细胞化学染色,分别测量视网膜神经元和RGCs的轴突长度,评价维生素B族是否有促进视网膜神经元和RGCs轴突再生伸长的神经营养作用;最后,观察视网膜神经元对低糖损伤的反应,及维生素B族对受损的细胞是否有保护作用。

These results can lead to the following conclusion:〤GRP synthesized by the motoneurons may be a self-serving neurotrophic factor after axotomy.(2) CGRP may serve as a "injury signal" activating both the central and peripherical gli

本研究结果提示,运动神经轴突损伤后,运动神经元合成分泌的CGRP对神经元自身具有直接的神经营养作用,CGRP还可以作为损伤神经元分泌的&损伤信号&激活中枢和外周胶质细胞,从而为运动神经元的存活和轴突再生创造有利的微环境。

Pinal cord injury is a devastating condition characterized by disruption of axonal connections, failure of axonal regeneration, and loss of motor and sensory function.

CI是以轴突连接的断裂、轴突再生不能、运动及感觉功能丢失为特征的综合症。

Myelinated fibers ca n be seen without a myelin, the nerve axons silk increase in the number of accumulati on; Pulpless axon fibers can be seen swelling injury earlier in myelinated fibers.

有髓纤维可见髓鞘不整,轴突内神经丝数量增加,堆积;无髓纤维可见轴突肿胀,损伤要早于有髓纤维。

The regenerated RGCs were labeled retrogradely with FluoroGold,and the size of regenerating RGCs body in each retina and the shape of axon in ON sections were observed under fluorescent microscope.

用荧光金逆行标记再生的轴突,在荧光镜下观察视网膜平铺片中再生的视网膜节细胞大小及视神经切片内再生的轴突

DRG co-cultured with sciatic nerve undergoing Wallerian degeneration for 14 days in vivo in serum free DMEM/F12,1 sciatic nerve segment was added into co-culture system at the first moment, the axons were strong and straight and could adhered to each other, and the number of axons dramatically reduced compared with that of DRG cultured alone in serum free medium.

分别在无血清和有血清的条件下将新生鼠的背根神经节和体内变性14天的大鼠坐骨神经段进行联合培养作为实验组,并以在相应条件下单独培养的DRG的轴突的生长情况作为对照组来观察比较轴突的生长情况。

Results Under our experimental conditions, three kinds of NO donors when in lower concentrations could not cause obvious changes of axons while when the concentration of DETA NONOate was doubled, it could induce the non-selective demyelination and Wallerian degeneration of both sensory and motor nerve fibers.

结果:在本实验条件下,应用三种NO供体的低浓度,都不能引起大鼠坐骨神经轴突的改变;将DETA NONOate浓度加倍则可导致轴突脱髓鞘和沃勒变性,但对有髓鞘纤维和无髓鞘纤维并无选择性作用。

2DRG co-cultured with sciatic nerve undergoing Wallerian degeneration for 14days in vivo in serum free DMEM/F12,1sciatic nerve segment was added into co-culture system at the first moment,the axons were strong and straight and could adhered to each other,and the number of axons dramatically reduced compared with that of DRG cultured alone in serum free medium.2DRG cultured in serum free DMEM/F12for 4days,then the sciatic nerve segment was added into the culture system for 3days,the axons became straight and part of them adhered to each other.

分别在无血清和有血清的条件下将新生鼠的背根神经节和体内变性14天的大鼠坐骨神经段进行联合培养作为实验组,并以在相应条件下单独培养的DRG的轴突的生长情况作为对照组来观察比较轴突的生长情况。

One simplified hypothesis is that, when the growth cone detected the directional signal, actin will polymerize asymmetrically in the growth cone, so that the filopodia and lamelipodia will extend preferentially to one side, microtubule will further follow and stabilize this growing direction.

一种假说认为,当轴突生长锥探测到方向性的信号时,生长锥内的 actin成分发生不对称聚合,filopodia及lamelipodia向一侧优先延伸,进而轴突内微管也朝同侧延伸,使生长方向进一步稳定下来。

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