轴突

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

A new antibody releasing system has been developed by covalently attachingIgG to the biodegradable hyaluronic acid hydrogel via the hydrolyticallyunstable hydrazone linkage, aiming to deliver the antibody of CNS regenerationinhibitors to the injured brain. Furthermore, pH sensitive linkage-hydrozone has beenformed between hydrogel and antibody.

这一将该材料植入成年缺损大鼠的大脑皮层后，该材料具有良好的神经相容性，能够抑制胶质瘢痕的形成，并能诱导神经胶质细胞的迁移进入材料，为轴突的再生提供良好的生物为环境，促进神经轴突长入将改性后的水凝胶。

That speed, in turn, depends a great deal on the myelination of the neuron's axons… The more myelin sheathes the axon, the faster the neuron can conduct its charge.

速度，在又取决于了大量关于髓鞘的神经元的神经轴突…更髓鞘sheathes轴突，更快的神经元可以进行收费。

Two types of pituicytes and three types of neurosecretory fibers (A1, A2 and B) have been recognized in neurohypophysis. The neurosecretory terminals contain different diameter and shape of secretory granules.

神经垂体组织中存在2种类型的脑垂体细胞，并可以区分A1、A2和B 3种类型的神经分泌纤维，其终端轴突中具有不同直径和形态的分泌颗粒；B型纤维轴突中含有许多透明小囊泡。

Through immunocytochemistry study, we found that the α3 and/or α5 subunits of nicotinic acetylcholine receptor are predominantly expressed in the oxytocinergic neurons. Our finding is quite different from the conclusion of the British scholar Dr. Mason's work in 1980s. We also found in this study that, the α3 and/or α5 subunits of N-AChR can also be expressed on the axons of oxytocinergic neurons, and these immunoreactive axons could be seen projecting to the median eminence and further to the posterior end of neurohypophysis. In the hypothalamus of the 7-day postnatal pup rats, the expression patterns of α3 and/or α5 subunits of N-AChR are similar to that in adult rats.

我们用免疫组化的方法发现，α3和/或α5型烟碱型乙酰胆碱受体(Nicotinic acetylcholine receptor，N-AChR)亚单位在大鼠下丘脑OT能神经元中呈优势性的表达，这与上世纪八十年代英国学者Mason类似工作的结论不一致；而且我们还发现α3和/或α5型N-AChR亚单位还表达在OT能神经元的轴突，此阳性免疫反应的轴突一直延续到正中隆起和神经垂体的末端；在新生7天大鼠的下丘脑，α3和/或α5型N-AChR亚单位的分布类似成年鼠。

The pituitary gland consists of neurohypophysis and adenohypophysis. Two types of pituicytes and three types of neurosecretory fibers (A1, A2 and B) have been recognized in neurohypophysis. The neurosecretory terminals contain different diameters and shape of secretory granules.

脑垂体由神经垂体和腺垂体2部分组成神经垂体组织中存在2种类型的脑垂体细胞，并可以区分A1型、A2型和B型3种类型的神经分泌纤维，其终端轴突中具有不同直径和形态的分泌颗粒；B型纤维轴突中含有许多透明小囊泡。

The results were as follows:The pituitary of Long-snout catfish included adenohypophysis and neurohypophysis. The adenohypophysis have six-cell types in common,i.e adrenocorticotro-phs,lactot-rophs,somatotrophs,gonadotrophs, thyrotrophs, and Melanotroph cells. In neurohypophysis, there were three types of neurosecretory fibres, types A1,A2 and B.There were different typesgranules in type A neurosecretory fibres axone.In type B neurosecretory fibres axone, there were many small transparent vesicles.

结果表明：长吻魷脑垂体包括腺垂体和神经垂体两个部分，腺垂体组织中有六种分泌细胞即促肾上腺皮质激素分泌细胞、催乳激素分泌细胞、生长激素分泌细胞、促性腺激素分泌细胞、促甲状腺激素分泌细胞、促黑色素细胞刺激激素分泌细胞；神经垂体组织中存在A型(A_1、A_2)和B型神经分泌纤维，A型分泌纤维轴突中具有不同类型的分泌颗粒，B型分泌纤维轴突中含有许多透明小囊泡。

The results were as follows: The pituitary of Long-snout catfish included adenohypophysis and neurohypophysis. The adenohypophysis have six-cell types in common,i.e adrenocorticotro-phs,lactot-rophs,somatotrophs,gonadotrophs, thyrotrophs, and Melanotroph cells. In neurohypophysis, there were three types of neurosecretory fibres, types A1,A2 and B.There were different types'granules in type A neurosecretory fibres axone.In type B neurosecretory fibres axone, there were many small transparent vesicles.

结果表明：长吻魷脑垂体包括腺垂体和神经垂体两个部分，腺垂体组织中有六种分泌细胞即促肾上腺皮质激素分泌细胞、催乳激素分泌细胞、生长激素分泌细胞、促性腺激素分泌细胞、促甲状腺激素分泌细胞、促黑色素细胞刺激激素分泌细胞；神经垂体组织中存在A型(A_1、A_2)和B型神经分泌纤维，A型分泌纤维轴突中具有不同类型的分泌颗粒，B型分泌纤维轴突中含有许多透明小囊泡。

The expression and axonal transport of pituitary adenylate ecelase-activing polypeptide was reported, The number of neurons immunoreactive for PACAP increased markedly within the nodose ganglion during a 24-48h culture period, PACAP was found to axonally transported and accumpulated at the site of a crush injury.

另有一个研究，是调查在体外培养的鼠迷走神经中垂体腺苷酸环化酶激活多肽的表达和轴突输送，提出在24-48小时的培养期，PACAP免疫反应性神经元的数目在结节神经节内显著地增加，PACAP参与轴突输送中并堆积在受损部位，在这部位被释放。

It was found greater expression of NRP2-1 and NRP2-2 in pituitary, ovary, uterus and oviduct of ruttish Erhualian than corresponding tissues of ruttish Duroc, which suggested enhancing expression of NRP2 was favorable to increasing litter size. But the difference of NRP2 expression was not significant between two breeds, which suggested the key phase that NRP2 affected litter size was not oestrum probably, or NRP2 could enlarge cell signals and finally affect litter size ascribe that it was the receptor of nerve axon guidance factors.

NRP2-1和NRP2-2在发情期二花脸卵巢、子宫、输卵管中的表达量均大于杜洛克相应组织的表达量，推测NRP2表达水平高可能有利于提高产仔数，但两品种间表达差异不明显，推测NRP2影响产仔数的关键阶段可能不是猪的发情期；另外，NRP2 是神经轴突介导因子的受体，这些神经轴突转导的信号往往有级联放大的功能，虽然NRP2-1和NRP2-2在二花脸和杜洛克卵巢、子宫、输卵管中的表达差异不大，但二花脸有可能通过神经系统转导信号的级联放大最终引起一些效应激素或蛋白的急剧增加，从而提高排卵率或产仔数。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷