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A trivalent plant expression vector containing Bt cry1Ah gene, cry1Ie gene and glyphosate-tolerant 2mG2-epsps gene was constructed, glyphosate isopropylamine salt as a screening agent, 2mG2-epsps gene as a selectable maker gene. The vector was transfer into maize immature embryonic calli by microprojectile bombardment, and 69 T0 generation plants were obtained. PCR analysis showed that 17 plants had the integration of insect-resistant cry1Ah, cry1Ie gene and glyphosate-tolerance 2mG2-epsps gene.

同时构建了含有Bt cry1Ah和cry1Ie基因和耐草甘膦2mG2-epsps基因的三价植物表达载体,通过基因枪轰击法转化玉米愈伤组织,以2mG2-epsps为筛选标记基因,以草甘膦异丙胺盐作为筛选剂,获得T0代转化植株69株,PCR检测结果表明:有17株已完整的整合有抗虫基因cry1Ah、cry1Ie和耐草甘膦基因2mG2-epsps的3个目的基因,RT-PCR分析外源基因可以正确转录,已获得结实转基因植株。

Fast atom bombardment mass spectrometry has been used to analyze the complex triglycerides in oils and fats of juglans mandshurica maxin.

本文利用快原子轰击质谱直接测定山核桃油中未经任何前处理的混合甘油三酸酯,能获得其分子量和碎片结构信息,在GC和GC/MS分析的基础上。

The dependence of the relative intensity of cationic molecule and fragmentation ion of an acetylated melezitose on the concentration of Na + or Li + in the matrix was studied by using fast atom bombardment source .

利用快原子轰击源研究了乙酰化松三糖的阳离子化分子和碎片离子的相对强度随底物中Na+或Li+浓度变化的趋势。

The analysis results of naphthenic acids were obtained by the use of MS and EI techniques.

通过电子轰击质谱分析得到的环烷酸。

The electron impact mass spectra of noncyclic crown ether s with heterocyclic methmazole as terminal groups 1a ̄4a and fo were given and discussed in the present article.

本文给出以杂环甲巯咪唑为端基的非环冠醚1a~4a及1a的异构体1b的电子轰击质谱并讨论之。

The native oxides on CdSe surface could be reduced to elemental Selenium by a HF etching process. Bombardment using 100eV Ar〓 ions with a dosage of about 1×10〓 ions/cm〓 could remove all the elemental Selenium, which results in a normal stoichiometrical surface CdSe.

实验观测到HF刻蚀可以将CdSe半导体表面的Se氧化物还原为元素Se.100eV的Ar〓离子轰击可以除去HF刻蚀后在表面还原生成的元素Se,从而清洁样品表面,保证了表面CdSe的化学计量比。

In this paper, the noise sources of the EBCCD imaging system have been analyzed and a SNR model has been established. It shows that the SNR of output image is related not only to the input illuminance on the cathode but also to the spatial frequency of the input image. This has been proved by a series of experiment. Unperfectness of the detecting equation has been pointed out in this paper.

分析了电子轰击电荷耦合器件微光成像系统的噪声源,建立了系统的信噪比模型,指出了在微光成像系统本身的增益和量子效率等性能确定的条件下,成像系统输出图像信噪比不仅仅与输入面照度有关,还与输入图像的空间频率有关,并通过实验得到了证明,指出了经典的图像探测方程的不完善之处。

"The goal is the ""attrit"" of his military powers down to half of what it was before the bombardment ."

城墙以内突起的土木工事可以大大减少大炮轰击的效果。

In 1945 the forts were manned by Japanese soldiers determined to hold out to the bitter end.

这些堡垒在二战中遭受到的日本军队的持续轰击导致了美国军队的投降。

Although it bashes non-governmental organisations, it has created a public chamber of approved and loyal members of the intelligentsia, which includes scientists, artists and lawyers.

尽管它对非政府机构猛烈的轰击,它创造出了一种公共活动室,忠诚的知识分子认可并成为了它的成员,包括科学家、艺术家和律师。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力